In situ correlation of synthesis and storage of parathormone in parathyroid gland disease

C. H. Kendall, Linda Potter, R. Brown, B. Jasani, J. H. Pringle, I. Lauder

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The distribution and expression of parathyroid hormone (PTH) were investigated in normal and abnormal parathyroid tissue. PTH was detected using a monoclonal antibody with specificity for the 44–68 region of the PTH molecule. Prominent reactivity for PTH was seen in normal parathyroid with a granular pattern of staining. Active parathyroid tissue (adenoma and hyperplasia) showed much less reactivity for PTH, although there was prominent reactivity in the normal tissue around adenomas. Comparison of expression of PTH with that of parathormone mRNA showed a reciprocal pauern in normal tissue and, to a less marked extent, in abnormal tissue. Parathyroid carcinoma in particular had coinciding areas of PTH and PTH mRNA expression. Oxyphil cells had little or no PTH expression, except in the associated ‘colloid’ in some cases. The findings indicate an inverse relationship between storage and cellular synthesis of PTH, this being more marked in physiological than in pathological conditions of the parathyroid.

Original languageEnglish
Pages (from-to)61-66
Number of pages6
JournalThe Journal of pathology
Volume169
Issue number1
DOIs
Publication statusPublished - Jan 1993

Keywords

  • Immunocytochemistry
  • Parathormone
  • in situ hybridization
  • messenger RNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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