In vivo analysis of CD4+ T cell responses to oral immunization

A. Saparov, L. A. Kraus, J. S. Marwill, R. P. Bucy, C. O. Elson, C. T. Weaver

Research output: Contribution to journalArticle

Abstract

An adoptive transfer model was used to study antigen-induced cytokinc expression, surface phenotype modulation, and anatomic localization of a clonal population of naive CD4 T cells in vivo following oral delivery of antigen. Ovalbumin (OVA)-specific CD4+ T cells isolated from DO 10 aβ TCRtransgenic mice were adoptively transferred into syngeneic (BALB/c) mice and OVA was delivered by gastric gavage in a multiple emulsion vehicle two days later. The clonotype-positive population of CD4 T cells in Peyer's patch (PP), mesenteric lymph node (MLN), and spleen (SP) were analyzed over a time course. Cytokine expression by transgenic lymphocytes in tissue cryosections was determined using an enzymatic in situ hybridization technique. PP cytokine responses were limited to infrequent IL-2 and IL-10 positive cells, and seen only at the earliest time points. MLN CD4+ T cells showed higher frequency expression of IL-2 and IL-10 and infrequent IFN-g at early time points. Cytokine responses in the spleen were delayed, and showed increased IFN-g relative to PP and MLN, but included significant numbers of IL-10 positive cells. This phenotype is distinct from delivery of OVA in the same vehicle I.P., where IL-2 and IFN-g were dominant, and IL-10 was rarely detected. Triple-color flow cytometric analysis of the transgenic T cell population showed increased expression of CD25, CD69 and CD40L compared to the endogenous, nontransgenic CD4+ T cell population. These data suggest that in vivo cytokinc responses are shifted by route of antigen delivery, and that primary CD4+ T cell responses are not limited to IL-2 in vivo.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number6
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Immunization
oral vaccination
T-cells
T-lymphocytes
T-Lymphocytes
interleukin-10
interleukin-2
Peyer's Patches
Interleukin-10
Peyer's patches
Interleukin-2
ovalbumin
Ovalbumin
lymph nodes
cytokines
Lymph Nodes
Cytokines
antigens
Antigens
Population

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Saparov, A., Kraus, L. A., Marwill, J. S., Bucy, R. P., Elson, C. O., & Weaver, C. T. (1996). In vivo analysis of CD4+ T cell responses to oral immunization. FASEB Journal, 10(6).

In vivo analysis of CD4+ T cell responses to oral immunization. / Saparov, A.; Kraus, L. A.; Marwill, J. S.; Bucy, R. P.; Elson, C. O.; Weaver, C. T.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

Saparov, A, Kraus, LA, Marwill, JS, Bucy, RP, Elson, CO & Weaver, CT 1996, 'In vivo analysis of CD4+ T cell responses to oral immunization', FASEB Journal, vol. 10, no. 6.
Saparov A, Kraus LA, Marwill JS, Bucy RP, Elson CO, Weaver CT. In vivo analysis of CD4+ T cell responses to oral immunization. FASEB Journal. 1996;10(6).
Saparov, A. ; Kraus, L. A. ; Marwill, J. S. ; Bucy, R. P. ; Elson, C. O. ; Weaver, C. T. / In vivo analysis of CD4+ T cell responses to oral immunization. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
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