Influence of enhanced electrogenicity on anodic biofilm and bioelectricity production by a novel microbial consortium

Obinna Markraphael Ajunwa, Olubusola Ayoola Odeniyi, Emmanuel Oluwaseun Garuba, Enrico Marsili, Abiodun Anthony Onilude

Research output: Contribution to journalArticlepeer-review

Abstract

Biofilms, flavins and pyocyanin have been identified as metabolites for species-specific electrogenicity. In this work, open circuit voltages (OCV) >500 mV earlier produced by electrogens in glucose-based media was used in confirming electrogens. Biofilms of test electrogens were grown on anodes of microbial fuel cells (MFCs) fed with glucose-based media. Electrogens used were anodophilic Enterobacter aerogenes 102, flavinogenic Bacillus sp. 101, Pichia kudriavzevii 103 and four pyocyanogenic Pseudomonads. Treatments with inhibitors (norspermidine, dichloro-diaminobenzene, and Raloxifene) negatively affected their electrogenicity. To enhance electrogenicity, increased anodic-biofilm was achieved by anode pretreatments with ultraviolet radiation and hydrogen peroxide, while pyocyanin-induced electrogenicity was enhanced by treatment with sophorolipds. Improved flavin yield and electrogenicity production was achieved by treatment with dimethyl-diaminobenzene. Bioelectricity production using metabolically-enhanced electrogens was carried out in a modified MFC and optimum yields were achieved using a consortium of all electrogens exposed to metabolic enhancements. Measurements of electrochemical potential showed improved electrogenicity in metabolically-enhanced consortium compared to the unenhanced consortium. Using eight MFCs in series, OCV value of 5000 mV was achieved, while parallel connections yielded increased current up to 6000 mA/m2. Electron microscopy showed well-developed biofilms formed by metabolically-enhanced consortium. EPS from consortium biofilm were also characterized using fourier transform-infrared (FT-IR) spectroscopy.

Original languageEnglish
Pages (from-to)27-38
Number of pages12
JournalProcess Biochemistry
Volume104
DOIs
Publication statusPublished - May 2021

Keywords

  • Anodic biofilm
  • Bioelectrochemistry
  • Exoelectrogenicity
  • Pyocyanin
  • Riboflavin

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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