Influence of pH and ionic strength on transmission of plasmid DNA through ultrafiltration membranes

E. Arkhangelsky, B. Steubing, E. Ben-Dov, A. Kushmaro, V. Gitis

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

One of the simple, robust and scalable methods for purification of plasmid DNA is by membrane filtration. Utilizing manipulation of pH, ionic strength, and membrane pore size, the process is designed to completely retain the plasmid DNA. The performed study shows that despite electrostatic repulsion and a significant size difference between plasmid and membrane pore, the circular double-stranded DNA plasmid is able to penetrate through UF membranes. Experiments demonstrate that the tertiary structure of DNA changes easily and is dramatically affected by variations in ionic strength and pH. The penetration is probably due to stretching of the plasmid by hydrodynamic shear. In contrast to previous reports, the transmitted DNA copies kept their integrity and infectious ability.

Original languageEnglish
Pages (from-to)111-119
Number of pages9
JournalDesalination
Volume227
Issue number1-3
DOIs
Publication statusPublished - Jul 30 2008
Externally publishedYes

Fingerprint

Ultrafiltration
ultrafiltration
Ionic strength
plasmid
Plasmids
DNA
membrane
Membranes
Stretching
Pore size
Purification
purification
Electrostatics
penetration
Hydrodynamics
hydrodynamics
experiment
Experiments

Keywords

  • Antiobiotic resistance
  • DNA vaccination
  • Gene transfer
  • Virus generation

ASJC Scopus subject areas

  • Filtration and Separation

Cite this

Influence of pH and ionic strength on transmission of plasmid DNA through ultrafiltration membranes. / Arkhangelsky, E.; Steubing, B.; Ben-Dov, E.; Kushmaro, A.; Gitis, V.

In: Desalination, Vol. 227, No. 1-3, 30.07.2008, p. 111-119.

Research output: Contribution to journalArticle

Arkhangelsky, E. ; Steubing, B. ; Ben-Dov, E. ; Kushmaro, A. ; Gitis, V. / Influence of pH and ionic strength on transmission of plasmid DNA through ultrafiltration membranes. In: Desalination. 2008 ; Vol. 227, No. 1-3. pp. 111-119.
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