Influence of pH and ionic strength on transmission of plasmid DNA through ultrafiltration membranes

E. Arkhangelsky; B. Steubing; E. Ben-Dov; A. Kushmaro; V. Gitis

doi:10.1016/j.desal.2007.07.017

Influence of pH and ionic strength on transmission of plasmid DNA through ultrafiltration membranes

E. Arkhangelsky, B. Steubing, E. Ben-Dov, A. Kushmaro, V. Gitis

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

One of the simple, robust and scalable methods for purification of plasmid DNA is by membrane filtration. Utilizing manipulation of pH, ionic strength, and membrane pore size, the process is designed to completely retain the plasmid DNA. The performed study shows that despite electrostatic repulsion and a significant size difference between plasmid and membrane pore, the circular double-stranded DNA plasmid is able to penetrate through UF membranes. Experiments demonstrate that the tertiary structure of DNA changes easily and is dramatically affected by variations in ionic strength and pH. The penetration is probably due to stretching of the plasmid by hydrodynamic shear. In contrast to previous reports, the transmitted DNA copies kept their integrity and infectious ability.

Original language	English
Pages (from-to)	111-119
Number of pages	9
Journal	Desalination
Volume	227
Issue number	1-3
DOIs	https://doi.org/10.1016/j.desal.2007.07.017
Publication status	Published - Jul 30 2008
Externally published	Yes

Keywords

Antiobiotic resistance
DNA vaccination
Gene transfer
Virus generation

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering
General Materials Science
Water Science and Technology
Mechanical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.desal.2007.07.017

Cite this

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title = "Influence of pH and ionic strength on transmission of plasmid DNA through ultrafiltration membranes",

abstract = "One of the simple, robust and scalable methods for purification of plasmid DNA is by membrane filtration. Utilizing manipulation of pH, ionic strength, and membrane pore size, the process is designed to completely retain the plasmid DNA. The performed study shows that despite electrostatic repulsion and a significant size difference between plasmid and membrane pore, the circular double-stranded DNA plasmid is able to penetrate through UF membranes. Experiments demonstrate that the tertiary structure of DNA changes easily and is dramatically affected by variations in ionic strength and pH. The penetration is probably due to stretching of the plasmid by hydrodynamic shear. In contrast to previous reports, the transmitted DNA copies kept their integrity and infectious ability.",

keywords = "Antiobiotic resistance, DNA vaccination, Gene transfer, Virus generation",

author = "E. Arkhangelsky and B. Steubing and E. Ben-Dov and A. Kushmaro and V. Gitis",

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AU - Arkhangelsky, E.

AU - Steubing, B.

AU - Ben-Dov, E.

AU - Kushmaro, A.

AU - Gitis, V.

N1 - Funding Information: This project was partially supported by grants from the The Stephen and Nancy Grand Water Research Institute (GWRI) to Elizabeth Arkhangelsky and BMBF-MOST to Bernhard Steubing. This work made use of the SPM facility of the R. Stadler Minerva center for mesoscale macromolecular engineering at BGU.

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AB - One of the simple, robust and scalable methods for purification of plasmid DNA is by membrane filtration. Utilizing manipulation of pH, ionic strength, and membrane pore size, the process is designed to completely retain the plasmid DNA. The performed study shows that despite electrostatic repulsion and a significant size difference between plasmid and membrane pore, the circular double-stranded DNA plasmid is able to penetrate through UF membranes. Experiments demonstrate that the tertiary structure of DNA changes easily and is dramatically affected by variations in ionic strength and pH. The penetration is probably due to stretching of the plasmid by hydrodynamic shear. In contrast to previous reports, the transmitted DNA copies kept their integrity and infectious ability.

KW - Antiobiotic resistance

KW - DNA vaccination

KW - Gene transfer

KW - Virus generation

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Influence of pH and ionic strength on transmission of plasmid DNA through ultrafiltration membranes

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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