Inhibition of Simian virus 40 large T antigen helicase activity by fluoroquinolones

Syed Hamid Ali, Anil Chandraker, James A. DeCaprio

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Background: Fluoroquinolones represent a potent group of antibiotics that inhibit bacterial DNA replication by targeting the essential bacterial enzymes gyrase and topoisomerase IV. Inhibition of gyrase activity by quinolones involves the interaction of these drugs with the helicase component of bacterial gyrase. DNA tumour viruses also encode helicases that are essential for their DNA replication in the host. Methods: In this study we have evaluated the effect of fluoroquinolones on viral DNA replication using the DNA tumour virus Simian virus 40 (SV40) as our model. Four different fluoroquinolones, namely, levofloxacin, trovafloxacin, ciprofloxacin and ofloxacin, were tested for their ability to inhibit viral DNA replication. Results: We show here that all four quinolones tested were effective in the inhibition of SV40 plaque formation and DNA replication in CV1-P cells. In addition, we found that each of these quinolones was inhibitory to the helicase activity of SV40 large tumour antigen. Conclusions: Fluoroquinolones and their derivates may therefore be useful in the treatment and/or prevention of infection by SV40-homologous human DNA viruses that encode helicase activity for their survival.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalAntiviral Therapy
Volume12
Issue number1
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

Simian virus 40
Viral Tumor Antigens
Fluoroquinolones
DNA Replication
Quinolones
DNA Tumor Viruses
Viral DNA
DNA Topoisomerase IV
Bacterial DNA
Levofloxacin
Ofloxacin
DNA Viruses
Neoplasm Antigens
Ciprofloxacin
Drug Interactions
Anti-Bacterial Agents
Survival
Enzymes
Infection

ASJC Scopus subject areas

  • Pharmacology

Cite this

Inhibition of Simian virus 40 large T antigen helicase activity by fluoroquinolones. / Ali, Syed Hamid; Chandraker, Anil; DeCaprio, James A.

In: Antiviral Therapy, Vol. 12, No. 1, 2007, p. 1-6.

Research output: Contribution to journalArticle

Ali, Syed Hamid ; Chandraker, Anil ; DeCaprio, James A. / Inhibition of Simian virus 40 large T antigen helicase activity by fluoroquinolones. In: Antiviral Therapy. 2007 ; Vol. 12, No. 1. pp. 1-6.
@article{82d7a5eab609403881dfaf9ed2825f27,
title = "Inhibition of Simian virus 40 large T antigen helicase activity by fluoroquinolones",
abstract = "Background: Fluoroquinolones represent a potent group of antibiotics that inhibit bacterial DNA replication by targeting the essential bacterial enzymes gyrase and topoisomerase IV. Inhibition of gyrase activity by quinolones involves the interaction of these drugs with the helicase component of bacterial gyrase. DNA tumour viruses also encode helicases that are essential for their DNA replication in the host. Methods: In this study we have evaluated the effect of fluoroquinolones on viral DNA replication using the DNA tumour virus Simian virus 40 (SV40) as our model. Four different fluoroquinolones, namely, levofloxacin, trovafloxacin, ciprofloxacin and ofloxacin, were tested for their ability to inhibit viral DNA replication. Results: We show here that all four quinolones tested were effective in the inhibition of SV40 plaque formation and DNA replication in CV1-P cells. In addition, we found that each of these quinolones was inhibitory to the helicase activity of SV40 large tumour antigen. Conclusions: Fluoroquinolones and their derivates may therefore be useful in the treatment and/or prevention of infection by SV40-homologous human DNA viruses that encode helicase activity for their survival.",
author = "Ali, {Syed Hamid} and Anil Chandraker and DeCaprio, {James A.}",
year = "2007",
language = "English",
volume = "12",
pages = "1--6",
journal = "Antiviral Therapy",
issn = "1359-6535",
publisher = "International Medical Press Ltd",
number = "1",

}

TY - JOUR

T1 - Inhibition of Simian virus 40 large T antigen helicase activity by fluoroquinolones

AU - Ali, Syed Hamid

AU - Chandraker, Anil

AU - DeCaprio, James A.

PY - 2007

Y1 - 2007

N2 - Background: Fluoroquinolones represent a potent group of antibiotics that inhibit bacterial DNA replication by targeting the essential bacterial enzymes gyrase and topoisomerase IV. Inhibition of gyrase activity by quinolones involves the interaction of these drugs with the helicase component of bacterial gyrase. DNA tumour viruses also encode helicases that are essential for their DNA replication in the host. Methods: In this study we have evaluated the effect of fluoroquinolones on viral DNA replication using the DNA tumour virus Simian virus 40 (SV40) as our model. Four different fluoroquinolones, namely, levofloxacin, trovafloxacin, ciprofloxacin and ofloxacin, were tested for their ability to inhibit viral DNA replication. Results: We show here that all four quinolones tested were effective in the inhibition of SV40 plaque formation and DNA replication in CV1-P cells. In addition, we found that each of these quinolones was inhibitory to the helicase activity of SV40 large tumour antigen. Conclusions: Fluoroquinolones and their derivates may therefore be useful in the treatment and/or prevention of infection by SV40-homologous human DNA viruses that encode helicase activity for their survival.

AB - Background: Fluoroquinolones represent a potent group of antibiotics that inhibit bacterial DNA replication by targeting the essential bacterial enzymes gyrase and topoisomerase IV. Inhibition of gyrase activity by quinolones involves the interaction of these drugs with the helicase component of bacterial gyrase. DNA tumour viruses also encode helicases that are essential for their DNA replication in the host. Methods: In this study we have evaluated the effect of fluoroquinolones on viral DNA replication using the DNA tumour virus Simian virus 40 (SV40) as our model. Four different fluoroquinolones, namely, levofloxacin, trovafloxacin, ciprofloxacin and ofloxacin, were tested for their ability to inhibit viral DNA replication. Results: We show here that all four quinolones tested were effective in the inhibition of SV40 plaque formation and DNA replication in CV1-P cells. In addition, we found that each of these quinolones was inhibitory to the helicase activity of SV40 large tumour antigen. Conclusions: Fluoroquinolones and their derivates may therefore be useful in the treatment and/or prevention of infection by SV40-homologous human DNA viruses that encode helicase activity for their survival.

UR - http://www.scopus.com/inward/record.url?scp=33847347546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847347546&partnerID=8YFLogxK

M3 - Article

VL - 12

SP - 1

EP - 6

JO - Antiviral Therapy

JF - Antiviral Therapy

SN - 1359-6535

IS - 1

ER -