Interaction between the integrin Mac-1 and signal regulatory protein α (SIRPα) mediates fusion in heterologous cells

Nataly P. Podolnikova, Marketa Hlavackova, Yifei Wu, Valentin P. Yakubenko, James Faust, Arnat Balabiyev, Xu Wang, Tatiana P. Ugarova

Research output: Contribution to journalArticle

Abstract

Macrophage fusion leading to the formation of multinucleated giant cells is a hallmark of chronic inflammation. Several membrane proteins have been implicated in mediating cell- cell attachment during fusion, but their binding partners remain unknown. Recently, we demonstrated that interleukin-4 (IL-4)- induced fusion of mouse macrophages depends on the integrin macrophage antigen 1 (Mac-1). Surprisingly, the genetic deficiency of intercellular adhesion molecule 1 (ICAM-1), an established ligand of Mac-1, did not impair macrophage fusion, suggesting the involvement of other counter-receptors. Here, using various approaches, including signal regulatory protein α (SIRPα) knockdown, recombinant proteins, adhesion and fusion assays, biolayer interferometry, and peptide libraries, we show that SIRPα, which, similar to ICAM-1, belongs to the Ig superfamily and has previously been implicated in cell fusion, interacts with Mac-1. The following results support the conclusion that SIRPα is a ligand of Mac-1: (a) recombinant ectodomain of SIRPα supports adhesion of Mac-1-expressing cells; (b) Mac-1-SIRPα interaction is mediated through the ligand-binding αMI-domain of Mac-1; (c) recognition of SIRPα by the αMIdomain conforms to general principles governing binding of Mac-1 to many of its ligands; (d) SIRPα reportedly binds CD47; however, anti-CD47 function-blocking mAb produced only a limited inhibition of macrophage adhesion to SIRPα; and (e) co-culturing of SIRPα- and Mac-1-expressing HEK293 cells resulted in the formation of multinucleated cells. Taken together, these results identify SIRPα as a counter-receptor for Mac-1 and suggest that the Mac-1-SIRPα interaction may be involved in macrophage fusion.

Original languageEnglish
Pages (from-to)7833-7849
Number of pages17
JournalJournal of Biological Chemistry
Volume294
Issue number19
DOIs
Publication statusPublished - Jan 1 2019

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Macrophage-1 Antigen
Integrins
Fusion reactions
Macrophages
Proteins
Ligands
Adhesion
Intercellular Adhesion Molecule-1
Interferometry
Recombinant Fusion Proteins
Peptide Library
Cell Fusion
HEK293 Cells
Giant Cells
Recombinant Proteins
Interleukin-4
Assays
Membrane Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Podolnikova, N. P., Hlavackova, M., Wu, Y., Yakubenko, V. P., Faust, J., Balabiyev, A., ... Ugarova, T. P. (2019). Interaction between the integrin Mac-1 and signal regulatory protein α (SIRPα) mediates fusion in heterologous cells. Journal of Biological Chemistry, 294(19), 7833-7849. https://doi.org/10.1074/jbc.RA118.006314

Interaction between the integrin Mac-1 and signal regulatory protein α (SIRPα) mediates fusion in heterologous cells. / Podolnikova, Nataly P.; Hlavackova, Marketa; Wu, Yifei; Yakubenko, Valentin P.; Faust, James; Balabiyev, Arnat; Wang, Xu; Ugarova, Tatiana P.

In: Journal of Biological Chemistry, Vol. 294, No. 19, 01.01.2019, p. 7833-7849.

Research output: Contribution to journalArticle

Podolnikova, NP, Hlavackova, M, Wu, Y, Yakubenko, VP, Faust, J, Balabiyev, A, Wang, X & Ugarova, TP 2019, 'Interaction between the integrin Mac-1 and signal regulatory protein α (SIRPα) mediates fusion in heterologous cells', Journal of Biological Chemistry, vol. 294, no. 19, pp. 7833-7849. https://doi.org/10.1074/jbc.RA118.006314
Podolnikova, Nataly P. ; Hlavackova, Marketa ; Wu, Yifei ; Yakubenko, Valentin P. ; Faust, James ; Balabiyev, Arnat ; Wang, Xu ; Ugarova, Tatiana P. / Interaction between the integrin Mac-1 and signal regulatory protein α (SIRPα) mediates fusion in heterologous cells. In: Journal of Biological Chemistry. 2019 ; Vol. 294, No. 19. pp. 7833-7849.
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AB - Macrophage fusion leading to the formation of multinucleated giant cells is a hallmark of chronic inflammation. Several membrane proteins have been implicated in mediating cell- cell attachment during fusion, but their binding partners remain unknown. Recently, we demonstrated that interleukin-4 (IL-4)- induced fusion of mouse macrophages depends on the integrin macrophage antigen 1 (Mac-1). Surprisingly, the genetic deficiency of intercellular adhesion molecule 1 (ICAM-1), an established ligand of Mac-1, did not impair macrophage fusion, suggesting the involvement of other counter-receptors. Here, using various approaches, including signal regulatory protein α (SIRPα) knockdown, recombinant proteins, adhesion and fusion assays, biolayer interferometry, and peptide libraries, we show that SIRPα, which, similar to ICAM-1, belongs to the Ig superfamily and has previously been implicated in cell fusion, interacts with Mac-1. The following results support the conclusion that SIRPα is a ligand of Mac-1: (a) recombinant ectodomain of SIRPα supports adhesion of Mac-1-expressing cells; (b) Mac-1-SIRPα interaction is mediated through the ligand-binding αMI-domain of Mac-1; (c) recognition of SIRPα by the αMIdomain conforms to general principles governing binding of Mac-1 to many of its ligands; (d) SIRPα reportedly binds CD47; however, anti-CD47 function-blocking mAb produced only a limited inhibition of macrophage adhesion to SIRPα; and (e) co-culturing of SIRPα- and Mac-1-expressing HEK293 cells resulted in the formation of multinucleated cells. Taken together, these results identify SIRPα as a counter-receptor for Mac-1 and suggest that the Mac-1-SIRPα interaction may be involved in macrophage fusion.

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