Interaction of imidazoline- and imidazolidine-based nitroxides with chloroplasts

V. V. Ptushenko, L. N. Ikryannikova, I. A. Grigor'ev, I. A. Kirilyuk, B. V. Trubitsin, Alexander N. Tikhonov

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


In this work, we investigated the interaction of a number of novel pH-sensitive spin probes (imidazoline- and imidazolidine-based nitroxides) with isolated chloroplasts. The light-induced changes in the electron paramagnetic resonance signals of these radicals are determined by several factors: ΔpH-driven accumulation of nitroxides inside the thylakoids, consumption (or evolution) of oxygen by chloroplasts, and irreversible loss of spin probe paramagnetism. A comparative study of these radicals characterized by different pKa allowed us to conclude that these spin probes can be used as pH indicators for monitoring the intrathylakoid pH- and light-induced changes in oxygen concentration in chloroplast suspension.

Original languageEnglish
Pages (from-to)329-343
Number of pages15
JournalApplied Magnetic Resonance
Issue number3-4
Publication statusPublished - Jan 1 2006
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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