Interaction of imidazoline- and imidazolidine-based nitroxides with chloroplasts

V. V. Ptushenko, L. N. Ikryannikova, I. A. Grigor'ev, I. A. Kirilyuk, B. V. Trubitsin, Alexander N. Tikhonov

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In this work, we investigated the interaction of a number of novel pH-sensitive spin probes (imidazoline- and imidazolidine-based nitroxides) with isolated chloroplasts. The light-induced changes in the electron paramagnetic resonance signals of these radicals are determined by several factors: ΔpH-driven accumulation of nitroxides inside the thylakoids, consumption (or evolution) of oxygen by chloroplasts, and irreversible loss of spin probe paramagnetism. A comparative study of these radicals characterized by different pKa allowed us to conclude that these spin probes can be used as pH indicators for monitoring the intrathylakoid pH- and light-induced changes in oxygen concentration in chloroplast suspension.

Original languageEnglish
Pages (from-to)329-343
Number of pages15
JournalApplied Magnetic Resonance
Volume30
Issue number3-4
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Imidazolidines
chloroplasts
Imidazolines
probes
pH factor
Oxygen
Paramagnetism
paramagnetism
interactions
oxygen
Paramagnetic resonance
Suspensions
electron paramagnetic resonance
Monitoring

ASJC Scopus subject areas

  • Spectroscopy
  • Atomic and Molecular Physics, and Optics

Cite this

Ptushenko, V. V., Ikryannikova, L. N., Grigor'ev, I. A., Kirilyuk, I. A., Trubitsin, B. V., & Tikhonov, A. N. (2006). Interaction of imidazoline- and imidazolidine-based nitroxides with chloroplasts. Applied Magnetic Resonance, 30(3-4), 329-343.

Interaction of imidazoline- and imidazolidine-based nitroxides with chloroplasts. / Ptushenko, V. V.; Ikryannikova, L. N.; Grigor'ev, I. A.; Kirilyuk, I. A.; Trubitsin, B. V.; Tikhonov, Alexander N.

In: Applied Magnetic Resonance, Vol. 30, No. 3-4, 2006, p. 329-343.

Research output: Contribution to journalArticle

Ptushenko, VV, Ikryannikova, LN, Grigor'ev, IA, Kirilyuk, IA, Trubitsin, BV & Tikhonov, AN 2006, 'Interaction of imidazoline- and imidazolidine-based nitroxides with chloroplasts', Applied Magnetic Resonance, vol. 30, no. 3-4, pp. 329-343.
Ptushenko VV, Ikryannikova LN, Grigor'ev IA, Kirilyuk IA, Trubitsin BV, Tikhonov AN. Interaction of imidazoline- and imidazolidine-based nitroxides with chloroplasts. Applied Magnetic Resonance. 2006;30(3-4):329-343.
Ptushenko, V. V. ; Ikryannikova, L. N. ; Grigor'ev, I. A. ; Kirilyuk, I. A. ; Trubitsin, B. V. ; Tikhonov, Alexander N. / Interaction of imidazoline- and imidazolidine-based nitroxides with chloroplasts. In: Applied Magnetic Resonance. 2006 ; Vol. 30, No. 3-4. pp. 329-343.
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