Interference Microscopy in Cell Biophysics. 1. Principles and Methodological Aspects of Coherent Phase Microscopy

Vladimir P. Tychinsky, Alexander N. Tikhonov

Research output: Contribution to journalReview article

11 Citations (Scopus)

Abstract

The purpose of our tandem publications is to review the applications of the coherent phase microscopy to cell biophysics. In this article, we briefly consider the fundamentals and methodological aspects of the coherent phase microscopy (CPM). One of important advantages of this method is a high sensitivity of CPM images to changes in physical-chemical properties of biological samples. The optical path length difference (OPLD), measured with CPM instruments in different domains of an object, serves as the informative optical parameter used for visualization of individual cells or intracellular organelles. Metabolically dependent variations of CPM images reflect changes in the functional state of biological objects. In the next article, we review results of CPM studies of different cells and intracellular energy-transducing organelles.

Original languageEnglish
Pages (from-to)107-116
Number of pages10
JournalCell Biochemistry and Biophysics
Volume58
Issue number3
DOIs
Publication statusPublished - Sep 3 2010
Externally publishedYes

Keywords

  • Coherent phase microscopy
  • Phase image processing
  • Phase thickness
  • Refractivity

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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