Interference Microscopy in Cell Biophysics. 2. Visualization of Individual Cells and Energy-Transducing Organelles

Vladimir P. Tychinsky; Alexander N. Tikhonov

doi:10.1007/s12013-010-9115-y

Interference Microscopy in Cell Biophysics. 2. Visualization of Individual Cells and Energy-Transducing Organelles

Vladimir P. Tychinsky, Alexander N. Tikhonov

Research output: Contribution to journal › Review article › peer-review

11 Citations (Scopus)

Abstract

The coherent phase microscopy (CPM) provides a convenient and non-invasive tool for imaging cells and intracellular organelles. In this article, we consider the applications of the CPM method to imaging different cells and energy-transducing intracellular organelles (mitochondria and chloroplasts). Experimental data presented below demonstrate that the optical path length difference of the object, which is the basic optical parameter measured by the CPM method, can serve as an indicator of metabolic states of different biological objects at cellular and subcellular levels of structural organization.

Original language	English
Pages (from-to)	117-128
Number of pages	12
Journal	Cell Biochemistry and Biophysics
Volume	58
Issue number	3
DOIs	https://doi.org/10.1007/s12013-010-9115-y
Publication status	Published - 2010
Externally published	Yes

Keywords

Cells
Chloroplasts
Coherent phase microscopy
Cyanobacteria
Membrane potential
Mitochondria
Refractivity
Spores

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1007/s12013-010-9115-y

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abstract = "The coherent phase microscopy (CPM) provides a convenient and non-invasive tool for imaging cells and intracellular organelles. In this article, we consider the applications of the CPM method to imaging different cells and energy-transducing intracellular organelles (mitochondria and chloroplasts). Experimental data presented below demonstrate that the optical path length difference of the object, which is the basic optical parameter measured by the CPM method, can serve as an indicator of metabolic states of different biological objects at cellular and subcellular levels of structural organization.",

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AU - Tychinsky, Vladimir P.

AU - Tikhonov, Alexander N.

PY - 2010

Y1 - 2010

N2 - The coherent phase microscopy (CPM) provides a convenient and non-invasive tool for imaging cells and intracellular organelles. In this article, we consider the applications of the CPM method to imaging different cells and energy-transducing intracellular organelles (mitochondria and chloroplasts). Experimental data presented below demonstrate that the optical path length difference of the object, which is the basic optical parameter measured by the CPM method, can serve as an indicator of metabolic states of different biological objects at cellular and subcellular levels of structural organization.

AB - The coherent phase microscopy (CPM) provides a convenient and non-invasive tool for imaging cells and intracellular organelles. In this article, we consider the applications of the CPM method to imaging different cells and energy-transducing intracellular organelles (mitochondria and chloroplasts). Experimental data presented below demonstrate that the optical path length difference of the object, which is the basic optical parameter measured by the CPM method, can serve as an indicator of metabolic states of different biological objects at cellular and subcellular levels of structural organization.

KW - Cells

KW - Chloroplasts

KW - Coherent phase microscopy

KW - Cyanobacteria

KW - Membrane potential

KW - Mitochondria

KW - Refractivity

KW - Spores

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U2 - 10.1007/s12013-010-9115-y

DO - 10.1007/s12013-010-9115-y

M3 - Review article

C2 - 20814832

AN - SCOPUS:77958456482

SN - 1085-9195

VL - 58

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EP - 128

JO - Cell Biochemistry and Biophysics

JF - Cell Biochemistry and Biophysics

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ER -

Interference Microscopy in Cell Biophysics. 2. Visualization of Individual Cells and Energy-Transducing Organelles

Abstract

Keywords

ASJC Scopus subject areas

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