Abstract
Single cell studies have identified intraclonal heterogeneity of cytokine production by activated T cells. To investigate implications of cytokine heterogeneity for cell fate, an interleukin (IL)-2 promoter-green fluorescent protein (GFP) reporter transgenic model was developed to track IL-2+ and IL-2- T cells during differentiation from naive precursors. Antigen-activated IL-2+ and IL-2- cells had comparable proliferative capacities in primary responses. However, T cells that expressed IL-2 in primary responses demonstrated enhanced antigenic sensitivity and increased expression of effector cytokines in secondary responses in vitro and in vivo. Thus, heterogeneity of activation during a primary response translates into heterogeneous secondary responses, in which enhanced memory/effector function is linked to cells that previously exceeded an activation threshold associated with IL-2 gene transcription.
| Original language | English |
|---|---|
| Pages (from-to) | 271-280 |
| Number of pages | 10 |
| Journal | Immunity |
| Volume | 11 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sep 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases
- Immunology
Cite this
Interleukin-2 expression by a subpopulation of primary T cells is linked to enhanced memory/effector function. / Saparov, Arman; Wagner, Fred H.; Zheng, Rui; Oliver, James R.; Maeda, Hiroko; Hockett, Richard D.; Weaver, Casey T.
In: Immunity, Vol. 11, No. 3, 09.1999, p. 271-280.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interleukin-2 expression by a subpopulation of primary T cells is linked to enhanced memory/effector function
AU - Saparov, Arman
AU - Wagner, Fred H.
AU - Zheng, Rui
AU - Oliver, James R.
AU - Maeda, Hiroko
AU - Hockett, Richard D.
AU - Weaver, Casey T.
PY - 1999/9
Y1 - 1999/9
N2 - Single cell studies have identified intraclonal heterogeneity of cytokine production by activated T cells. To investigate implications of cytokine heterogeneity for cell fate, an interleukin (IL)-2 promoter-green fluorescent protein (GFP) reporter transgenic model was developed to track IL-2+ and IL-2- T cells during differentiation from naive precursors. Antigen-activated IL-2+ and IL-2- cells had comparable proliferative capacities in primary responses. However, T cells that expressed IL-2 in primary responses demonstrated enhanced antigenic sensitivity and increased expression of effector cytokines in secondary responses in vitro and in vivo. Thus, heterogeneity of activation during a primary response translates into heterogeneous secondary responses, in which enhanced memory/effector function is linked to cells that previously exceeded an activation threshold associated with IL-2 gene transcription.
AB - Single cell studies have identified intraclonal heterogeneity of cytokine production by activated T cells. To investigate implications of cytokine heterogeneity for cell fate, an interleukin (IL)-2 promoter-green fluorescent protein (GFP) reporter transgenic model was developed to track IL-2+ and IL-2- T cells during differentiation from naive precursors. Antigen-activated IL-2+ and IL-2- cells had comparable proliferative capacities in primary responses. However, T cells that expressed IL-2 in primary responses demonstrated enhanced antigenic sensitivity and increased expression of effector cytokines in secondary responses in vitro and in vivo. Thus, heterogeneity of activation during a primary response translates into heterogeneous secondary responses, in which enhanced memory/effector function is linked to cells that previously exceeded an activation threshold associated with IL-2 gene transcription.
UR - http://www.scopus.com/inward/record.url?scp=0033199990&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033199990&partnerID=8YFLogxK
U2 - 10.1016/S1074-7613(00)80102-8
DO - 10.1016/S1074-7613(00)80102-8
M3 - Article
C2 - 10514005
AN - SCOPUS:0033199990
VL - 11
SP - 271
EP - 280
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 3
ER -