Investigating the inhibitory effect of cyanide, phenol and 4-nitrophenol on the activated sludge process employed for the treatment of petroleum wastewater

V J Inglezakis, S Malamis, A Omirkhan, J Nauruzbayeva, Z Makhtayeva, T Seidakhmetov, A Kudarova

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Abstract

In this work, the inhibitory effect of cyanide, phenol and 4-nitrophenol on the activated sludge process was investigated. The inhibition of the aerobic oxidation of organic matter, nitrification and denitrification were examined in batch reactors by measuring the specific oxygen uptake rate (sOUR), the specific ammonium uptake rate (sAUR) and the specific nitrogen uptake rate (sNUR) respectively. The tested cyanide, phenol and 4-nitrophenol concentrations were 0.2-1.7 mg/L, 4.8-73.1 mg/L and 8.2-73.0 mg/L respectively. Cyanide was highly toxic as it significantly (>50%) inhibited the activity of autotrophic biomass, heterotrophic biomass under aerobic conditions and denitrifiers even at relatively low concentrations (1.0-1.7 mgCN(-)/L). The determination of the half maximum inhibitory concentration (IC50) confirmed this, since for cyanide IC50 values were very low for the examined bioprocesses (<1.5 mg/L). On the other hand, the IC50 values for phenol and 4-nitrophenol were much higher (>25 mg/L) for the tested bioprocesses since appreciable concentrations were required to accomplish significant inhibition. The autotrophic bacteria were more sensitive to phenol than the aerobic heterotrophs. The denitrifiers were found to be very resistant to phenol.

Original languageEnglish
Pages (from-to)825-830
Number of pages6
JournalJournal of Environmental Management
Volume203
Issue numberPt 2
DOIs
Publication statusPublished - Dec 1 2017

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Activated sludge process
Cyanides
cyanide
Phenols
activated sludge
phenol
Wastewater
Crude oil
petroleum
wastewater
Biomass
Nitrification
Denitrification
biomass
Batch reactors
oxic conditions
Biological materials
nitrification
denitrification
Bacteria

Keywords

  • Journal Article

Cite this

Investigating the inhibitory effect of cyanide, phenol and 4-nitrophenol on the activated sludge process employed for the treatment of petroleum wastewater. / Inglezakis, V J; Malamis, S; Omirkhan, A; Nauruzbayeva, J; Makhtayeva, Z; Seidakhmetov, T; Kudarova, A.

In: Journal of Environmental Management, Vol. 203, No. Pt 2, 01.12.2017, p. 825-830.

Research output: Contribution to journalArticle

Inglezakis, V J ; Malamis, S ; Omirkhan, A ; Nauruzbayeva, J ; Makhtayeva, Z ; Seidakhmetov, T ; Kudarova, A. / Investigating the inhibitory effect of cyanide, phenol and 4-nitrophenol on the activated sludge process employed for the treatment of petroleum wastewater. In: Journal of Environmental Management. 2017 ; Vol. 203, No. Pt 2. pp. 825-830.
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AU - Nauruzbayeva, J

AU - Makhtayeva, Z

AU - Seidakhmetov, T

AU - Kudarova, A

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N2 - In this work, the inhibitory effect of cyanide, phenol and 4-nitrophenol on the activated sludge process was investigated. The inhibition of the aerobic oxidation of organic matter, nitrification and denitrification were examined in batch reactors by measuring the specific oxygen uptake rate (sOUR), the specific ammonium uptake rate (sAUR) and the specific nitrogen uptake rate (sNUR) respectively. The tested cyanide, phenol and 4-nitrophenol concentrations were 0.2-1.7 mg/L, 4.8-73.1 mg/L and 8.2-73.0 mg/L respectively. Cyanide was highly toxic as it significantly (>50%) inhibited the activity of autotrophic biomass, heterotrophic biomass under aerobic conditions and denitrifiers even at relatively low concentrations (1.0-1.7 mgCN(-)/L). The determination of the half maximum inhibitory concentration (IC50) confirmed this, since for cyanide IC50 values were very low for the examined bioprocesses (<1.5 mg/L). On the other hand, the IC50 values for phenol and 4-nitrophenol were much higher (>25 mg/L) for the tested bioprocesses since appreciable concentrations were required to accomplish significant inhibition. The autotrophic bacteria were more sensitive to phenol than the aerobic heterotrophs. The denitrifiers were found to be very resistant to phenol.

AB - In this work, the inhibitory effect of cyanide, phenol and 4-nitrophenol on the activated sludge process was investigated. The inhibition of the aerobic oxidation of organic matter, nitrification and denitrification were examined in batch reactors by measuring the specific oxygen uptake rate (sOUR), the specific ammonium uptake rate (sAUR) and the specific nitrogen uptake rate (sNUR) respectively. The tested cyanide, phenol and 4-nitrophenol concentrations were 0.2-1.7 mg/L, 4.8-73.1 mg/L and 8.2-73.0 mg/L respectively. Cyanide was highly toxic as it significantly (>50%) inhibited the activity of autotrophic biomass, heterotrophic biomass under aerobic conditions and denitrifiers even at relatively low concentrations (1.0-1.7 mgCN(-)/L). The determination of the half maximum inhibitory concentration (IC50) confirmed this, since for cyanide IC50 values were very low for the examined bioprocesses (<1.5 mg/L). On the other hand, the IC50 values for phenol and 4-nitrophenol were much higher (>25 mg/L) for the tested bioprocesses since appreciable concentrations were required to accomplish significant inhibition. The autotrophic bacteria were more sensitive to phenol than the aerobic heterotrophs. The denitrifiers were found to be very resistant to phenol.

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