Investigation of transmembrane protein fused in lipid bilayer membranes supported on porous silicon

Khalid Hasan Tantawi, Ramon Cerro, Bakhrom Berdiev, M. Elena Diaz Martin, Francisco Javier Montes, Darayas Patel, John D. Williams

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Abstract

This article investigates a device made from a porous silicon structure supporting a lipid bilayer membrane (LBM)fused with Epithelial Sodium Channel protein. The electrochemically-fabricated porous silicon template had pore diameters in the range 0.2~2 μm. Membranes were composed of two synthetic phospholipids: 1,2-diphytanoyl-sn-glycero-3-phosphoserine and 1,2-diphytanoyl-sn-glycero-3-phosphoethanolamine. The LBMwas formed by means of the Langmuir-Blodgett and Langmuir-Schaefer techniques, at a monolayer surface tension of 26 m Nm-1 in room temperature and on a deionized water subphase, which resulted in an average molecular area of 0.68-0.73 nm 2. Fusion of transmembrane protein was investigated using Atomic Force Microscopy. Initial atomic force microscopy results demonstrate the ability to support lipid bilayers fused with transmembrane proteins across a porous silicon substrate. However, more control of the membrane's surface tension using traditional membrane fusion techniques is required to optimize protein incorporation.

Original languageEnglish
Pages (from-to)28-34
Number of pages7
JournalJournal of Medical Engineering and Technology
Volume37
Issue number1
DOIs
Publication statusPublished - Jan 1 2013

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Keywords

  • Lipid bilayer membranes
  • Porous silicon
  • Transmembrane protein

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Tantawi, K. H., Cerro, R., Berdiev, B., Martin, M. E. D., Montes, F. J., Patel, D., & Williams, J. D. (2013). Investigation of transmembrane protein fused in lipid bilayer membranes supported on porous silicon. Journal of Medical Engineering and Technology, 37(1), 28-34. https://doi.org/10.3109/03091902.2012.733056