Investigation of transmembrane protein fused in lipid bilayer membranes supported on porous silicon

Khalid Hasan Tantawi, Ramon Cerro, Bakhrom Berdiev, M. Elena Diaz Martin, Francisco Javier Montes, Darayas Patel, John D. Williams

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This article investigates a device made from a porous silicon structure supporting a lipid bilayer membrane (LBM)fused with Epithelial Sodium Channel protein. The electrochemically-fabricated porous silicon template had pore diameters in the range 0.2~2 μm. Membranes were composed of two synthetic phospholipids: 1,2-diphytanoyl-sn-glycero-3-phosphoserine and 1,2-diphytanoyl-sn-glycero-3-phosphoethanolamine. The LBMwas formed by means of the Langmuir-Blodgett and Langmuir-Schaefer techniques, at a monolayer surface tension of 26 m Nm-1 in room temperature and on a deionized water subphase, which resulted in an average molecular area of 0.68-0.73 nm 2. Fusion of transmembrane protein was investigated using Atomic Force Microscopy. Initial atomic force microscopy results demonstrate the ability to support lipid bilayers fused with transmembrane proteins across a porous silicon substrate. However, more control of the membrane's surface tension using traditional membrane fusion techniques is required to optimize protein incorporation.

Original languageEnglish
Pages (from-to)28-34
Number of pages7
JournalJournal of Medical Engineering and Technology
Volume37
Issue number1
DOIs
Publication statusPublished - Jan 2013
Externally publishedYes

Fingerprint

Lipid bilayers
Porous silicon
Lipid Bilayers
Silicon
Proteins
Membranes
Surface Tension
Atomic Force Microscopy
Surface tension
Atomic force microscopy
Fusion reactions
Epithelial Sodium Channels
Phosphoserine
Membrane Fusion
Deionized water
Phospholipids
Monolayers
Sodium
Equipment and Supplies
Temperature

Keywords

  • Lipid bilayer membranes
  • Porous silicon
  • Transmembrane protein

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine(all)

Cite this

Tantawi, K. H., Cerro, R., Berdiev, B., Martin, M. E. D., Montes, F. J., Patel, D., & Williams, J. D. (2013). Investigation of transmembrane protein fused in lipid bilayer membranes supported on porous silicon. Journal of Medical Engineering and Technology, 37(1), 28-34. https://doi.org/10.3109/03091902.2012.733056

Investigation of transmembrane protein fused in lipid bilayer membranes supported on porous silicon. / Tantawi, Khalid Hasan; Cerro, Ramon; Berdiev, Bakhrom; Martin, M. Elena Diaz; Montes, Francisco Javier; Patel, Darayas; Williams, John D.

In: Journal of Medical Engineering and Technology, Vol. 37, No. 1, 01.2013, p. 28-34.

Research output: Contribution to journalArticle

Tantawi, Khalid Hasan ; Cerro, Ramon ; Berdiev, Bakhrom ; Martin, M. Elena Diaz ; Montes, Francisco Javier ; Patel, Darayas ; Williams, John D. / Investigation of transmembrane protein fused in lipid bilayer membranes supported on porous silicon. In: Journal of Medical Engineering and Technology. 2013 ; Vol. 37, No. 1. pp. 28-34.
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