Investigations on transmembrane ion channels suspended over porous silicon membranes

Khalid Hasan Tantawi, Ramon Cerro, Bakhrom Berdiev, John D. Williams

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This work presents a method to characterize the electrochemical properties of transmembrane ion channels and lipid bilayer membranes. The system is composed of a 3 μm thick porous silicon membrane with the epithelial sodium channel (ENaC) proteins fused into a lipid bilayer membrane (LBM) supported on the porous silicon layer. The LBM was composed of two synthetic phospholipids: 1,2-diphytanoyl-sn-glycero-3-phosphoserine and 1,2-diphytanoyl-sn-glycero-3- phosphoethanolamine. Electrical Impedance spectroscopy was performed from 0.1 Hz to 100 KHz. The electrochemically-fabricated porous silicon template had pore diameters in the range 0.2-2 urn. The LBM was formed by means of the Langmuir-Blodgett and Langmuir-Schaefer techniques, at a bilayer surface tension of 40 mN/m in room temperature. The electrolyte-PSi system showed on average a capacitance of about 9.76 μF/cm". The lipid bilayer membrane showed a capacitance of about 0.63 μF/cm2. The ENaC channels resulted in a capacitance of about 0.57 μF/cm".

Original languageEnglish
Title of host publicationTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Pages198-201
Number of pages4
Publication statusPublished - Aug 9 2013
EventNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 - Washington, DC, United States
Duration: May 12 2013May 16 2013

Publication series

NameTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Volume3

Other

OtherNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
CountryUnited States
CityWashington, DC
Period5/12/135/16/13

Keywords

  • Electrical impedance
  • Lipid Bilayer Membranes
  • Porous silicon
  • Transmembrane proteins

ASJC Scopus subject areas

  • Biotechnology

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  • Cite this

    Tantawi, K. H., Cerro, R., Berdiev, B., & Williams, J. D. (2013). Investigations on transmembrane ion channels suspended over porous silicon membranes. In Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 (pp. 198-201). (Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013; Vol. 3).