This work presents a method to characterize the electrochemical properties of transmembrane ion channels and lipid bilayer membranes. The system is composed of a 3 μm thick porous silicon membrane with the epithelial sodium channel (ENaC) proteins fused into a lipid bilayer membrane (LBM) supported on the porous silicon layer. The LBM was composed of two synthetic phospholipids: 1,2-diphytanoyl-sn-glycero-3-phosphoserine and 1,2-diphytanoyl-sn-glycero-3- phosphoethanolamine. Electrical Impedance spectroscopy was performed from 0.1 Hz to 100 KHz. The electrochemically-fabricated porous silicon template had pore diameters in the range 0.2-2 urn. The LBM was formed by means of the Langmuir-Blodgett and Langmuir-Schaefer techniques, at a bilayer surface tension of 40 mN/m in room temperature. The electrolyte-PSi system showed on average a capacitance of about 9.76 μF/cm". The lipid bilayer membrane showed a capacitance of about 0.63 μF/cm2. The ENaC channels resulted in a capacitance of about 0.57 μF/cm".