ISH-basierte HER2-Diagnostik

Translated title of the contribution: ISH-based HER2 diagnostics

Josef Rüschoff, Iris Nagelmeier, Bharat Jasani, Oliver Stoss

Research output: Contribution to journalReview articlepeer-review

Abstract

A prerequisite for all HER2 directed therapies is the demonstration of HER2 receptor protein overexpression and/or gene amplification by in situ hybridization (ISH). ASCO and CAP have published several HER2 test guidelines over the past 15 years for both breast and gastric cancer. The latest version for breast cancer (2018) focuses on special issues of ISH related to the definitions of special diagnostic groups (1–5). The guidelines for gastroesophageal adenocarcinoma (2017), essentially based on ToGA trial data, are now also being used for other tumors such as pancreas, gallbladder, and non-small-cell lung cancer. For colorectal cancer, a modified testing procedure has been proposed. Recently, besides overexpression and amplification, a third type of HER gene alteration, namely mutation, has gained much interest. Next-generation sequencing (NGS) allows detection of both amplification and mutation of the HER2 gene providing new options of therapy especially in the case of activating mutations.

Translated title of the contributionISH-based HER2 diagnostics
Original languageGerman
Pages (from-to)606-613
Number of pages8
JournalPathologe
Volume41
Issue number6
DOIs
Publication statusPublished - Nov 1 2020

Keywords

  • HER2 diagnostics
  • Immunohistochemistry
  • Next generation sequencing
  • Testing guidelines
  • in situ hybridization

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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