Isolation and purification of DNA from complicated biological samples

Ruslan Kalendar, Svetlana Boronnikova, Mervi M. Seppänen

Research output: Contribution to journalBook/Film/Article reviewpeer-review

1 Citation (Scopus)

Abstract

The isolation of nucleic acids from a biological sample is an important step for many molecular biology applications and medical diagnostic assays. This chapter describes an efficient protocol using established acidic CTAB (with a pH value of 5.0 to 6.8) based extraction method for isolation and/or purification of high molecular weight genomic DNA from a range of fresh and difficult sources from plant, animal, fungi and soil material. This protocol is suitable for many sequencing and genotyping applications, including large-scale sample screening.
Original languageEnglish
Pages (from-to) 57-67
JournalMethods in Molecular Biology
Volume2222
Issue number2
DOIs
Publication statusPublished - Mar 25 2021

Fingerprint Dive into the research topics of 'Isolation and purification of DNA from complicated biological samples'. Together they form a unique fingerprint.

Cite this