Isolation and purification of DNA from complicated biological samples

Ruslan Kalendar, Svetlana Boronnikova, Mervi M. Seppänen

Research output: Contribution to journalBook/Film/Article reviewpeer-review

27 Citations (Scopus)
1 Downloads (Pure)


The isolation of nucleic acids from a biological sample is an important step for many molecular biology applications and medical diagnostic assays. This chapter describes an efficient protocol using established acidic CTAB (with a pH value of 5.0 to 6.8) based extraction method for isolation and/or purification of high molecular weight genomic DNA from a range of fresh and difficult sources from plant, animal, fungi and soil material. This protocol is suitable for many sequencing and genotyping applications, including large-scale sample screening.
Original languageEnglish
Pages (from-to) 57-67
JournalMethods in Molecular Biology
Issue number2
Publication statusPublished - Mar 25 2021


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