TY - JOUR
T1 - Isolation and purification of DNA from complicated biological samples
AU - Kalendar, Ruslan
AU - Boronnikova, Svetlana
AU - Seppänen, Mervi M.
PY - 2021/3/25
Y1 - 2021/3/25
N2 - The isolation of nucleic acids from a biological sample is an important step for many molecular biology applications and medical diagnostic assays. This chapter describes an efficient protocol using established acidic CTAB (with a pH value of 5.0 to 6.8) based extraction method for isolation and/or purification of high molecular weight genomic DNA from a range of fresh and difficult sources from plant, animal, fungi and soil material. This protocol is suitable for many sequencing and genotyping applications, including large-scale sample screening.
AB - The isolation of nucleic acids from a biological sample is an important step for many molecular biology applications and medical diagnostic assays. This chapter describes an efficient protocol using established acidic CTAB (with a pH value of 5.0 to 6.8) based extraction method for isolation and/or purification of high molecular weight genomic DNA from a range of fresh and difficult sources from plant, animal, fungi and soil material. This protocol is suitable for many sequencing and genotyping applications, including large-scale sample screening.
UR - https://www.springer.com/gp/book/9781071609965
UR - https://link.springer.com/book/10.1007%2F978-1-0716-0997-2
U2 - 10.1007/978-1-0716-0997-2_3
DO - 10.1007/978-1-0716-0997-2_3
M3 - Book/Film/Article review
SN - 1064-3745
VL - 2222
SP - 57
EP - 67
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
IS - 2
ER -
