Isolation and purification of DNA from complicated biological samples

Ruslan Kalendar; Svetlana  Boronnikova; Mervi M. Seppänen

doi:10.1007/978-1-0716-0997-2_3

Isolation and purification of DNA from complicated biological samples

Ruslan Kalendar
, Svetlana Boronnikova
, Mervi M. Seppänen

Laboratory of bioinformatics and systems biology

Perm State National Research University
University of Helsinki

Research output: Contribution to journal › Book/Film/Article review › peer-review

52 Citations (Scopus)

1 Downloads (Pure)

Abstract

The isolation of nucleic acids from a biological sample is an important step for many molecular biology applications and medical diagnostic assays. This chapter describes an efficient protocol using established acidic CTAB (with a pH value of 5.0 to 6.8) based extraction method for isolation and/or purification of high molecular weight genomic DNA from a range of fresh and difficult sources from plant, animal, fungi and soil material. This protocol is suitable for many sequencing and genotyping applications, including large-scale sample screening.

Original language	English
Pages (from-to)	57-67
Number of pages	11
Journal	Methods in Molecular Biology
Volume	2222
Issue number	2
DOIs	https://doi.org/10.1007/978-1-0716-0997-2_3
Publication status	Published - Mar 25 2021

Funding

The work was partially supported by the Government of Perm Krai, research project No ?-26/174.3 on 31.01.2019.

Funders	Funder number
Perm Krai Government	-26/174.3

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 1 No Poverty
SDG 6 Clean Water and Sanitation
SDG 12 Responsible Consumption and Production

Keywords

Animal tissues
CTAB
DNA extraction
Food
Herbarium specimens
High-quality DNA
Plant tissues
Soil
Woody plants

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-0716-0997-2_3Licence: CC BY-ND

https://pubmed.ncbi.nlm.nih.gov/33301087/Licence: CC BY-ND

Cite this

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title = "Isolation and purification of DNA from complicated biological samples",

abstract = "The isolation of nucleic acids from a biological sample is an important step for many molecular biology applications and medical diagnostic assays. This chapter describes an efficient protocol using established acidic CTAB (with a pH value of 5.0 to 6.8) based extraction method for isolation and/or purification of high molecular weight genomic DNA from a range of fresh and difficult sources from plant, animal, fungi and soil material. This protocol is suitable for many sequencing and genotyping applications, including large-scale sample screening.",

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AU - Kalendar, Ruslan

AU - Boronnikova, Svetlana

AU - Seppänen, Mervi M.

PY - 2021/3/25

Y1 - 2021/3/25

N2 - The isolation of nucleic acids from a biological sample is an important step for many molecular biology applications and medical diagnostic assays. This chapter describes an efficient protocol using established acidic CTAB (with a pH value of 5.0 to 6.8) based extraction method for isolation and/or purification of high molecular weight genomic DNA from a range of fresh and difficult sources from plant, animal, fungi and soil material. This protocol is suitable for many sequencing and genotyping applications, including large-scale sample screening.

AB - The isolation of nucleic acids from a biological sample is an important step for many molecular biology applications and medical diagnostic assays. This chapter describes an efficient protocol using established acidic CTAB (with a pH value of 5.0 to 6.8) based extraction method for isolation and/or purification of high molecular weight genomic DNA from a range of fresh and difficult sources from plant, animal, fungi and soil material. This protocol is suitable for many sequencing and genotyping applications, including large-scale sample screening.

KW - Animal tissues

KW - CTAB

KW - DNA extraction

KW - Food

KW - Herbarium specimens

KW - High-quality DNA

KW - Plant tissues

KW - Soil

KW - Woody plants

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UR - https://www.scopus.com/pages/publications/85097813991#tab=citedBy

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DO - 10.1007/978-1-0716-0997-2_3

M3 - Book/Film/Article review

SN - 1064-3745

VL - 2222

SP - 57

EP - 67

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

IS - 2

ER -

Isolation and purification of DNA from complicated biological samples

Abstract

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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