Isolation of a strong promoter and its functional study

Ying Qiu Xie, Zhen Zhu, Qian Wu, Hong Lin Xu, Meng Meng, Yu Le Liu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A bidirectional promoter of cotton leaf curl virus (CLCuV) was obtained from the total of DNA CLCuV infected tomato leaves by polymerase chain reaction, and the amplified DNA fragment was cloned into the vector. DNA sequences analysis and homology comparison with the promoter of four kinds of isolates recently found indicated that the cloned promoter fragment composed of 436 bp was 99.32% homolog was up to in nucleotides with that of the isolates. Transient expression vectors were constructed by fusing the promoter fragment with gus reporter gene and nopaline terminator in different orientation. These constructs were delivered into the tobacco (Nicotiana tabacum L.) and cotton (Gossypium hirsutum L.) leaf cells for transient expression by particle bombardment. The results indicated that complementary sense promoter was a strong promoter with high activity in leaf mesophyll and vascular tissues, but virion sense promoter was weaker. The experiments suggested that isolated bidirectional promoter, as a novel strong promoter, could be used for dicots and especially cotton genetic transformation.

Original languageEnglish
Pages (from-to)50-54
Number of pages5
JournalActa Botanica Sinica
Volume42
Issue number1
Publication statusPublished - Jan 1 2000

Keywords

  • Cotton
  • Geminivirus
  • Particle bombardment
  • Promoter
  • Tobacco

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

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    Xie, Y. Q., Zhu, Z., Wu, Q., Xu, H. L., Meng, M., & Liu, Y. L. (2000). Isolation of a strong promoter and its functional study. Acta Botanica Sinica, 42(1), 50-54.