Isolation of blood-vessel-derived multipotent precursors from human skeletal muscle

William C W Chen, Arman Saparov, Mirko Corselli, Mihaela Crisan, Bo Zheng, Bruno Péault, Johnny Huard

Research output: Contribution to journalArticle

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Abstract

Since the discovery of mesenchymal stem/stromal cells (MSCs), the native identity and localization of MSCs have been obscured by their retrospective isolation in culture. Recently, using fluorescence-activated cell sorting (FACS), we and other researchers prospectively identified and purified three subpopulations of multipotent precursor cells associated with the vasculature of human skeletal muscle. These three cell populations: myogenic endothelial cells (MECs), pericytes (PCs), and adventitial cells (ACs), are localized respectively to the three structural layers of blood vessels: intima, media, and adventitia. All of these human blood-vessel-derived stem cell (hBVSC) populations not only express classic MSC markers but also possess mesodermal developmental potentials similar to typical MSCs. Previously, MECs, PCs, and ACs have been isolated through distinct protocols and subsequently characterized in separate studies. The current isolation protocol, through modifications to the isolation process and adjustments in the selective cell surface markers, allows us to simultaneously purify all three hBVSC subpopulations by FACS from a single human muscle biopsy. This new method will not only streamline the isolation of multiple BVSC subpopulations but also facilitate future clinical applications of hBVSCs for distinct therapeutic purposes.

LanguageEnglish
Pagese51195
JournalJournal of Visualized Experiments
Issue number90
DOIs
StatePublished - 2014
Externally publishedYes

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Blood vessels
Stem cells
Mesenchymal Stromal Cells
Blood Vessels
Muscle
Skeletal Muscle
Adventitia
Endothelial cells
Sorting
Pericytes
Fluorescence
Cells
Flow Cytometry
Stem Cells
Endothelial Cells
Biopsy
Cell culture
Population
Research Personnel
Muscles

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Chen, W. C. W., Saparov, A., Corselli, M., Crisan, M., Zheng, B., Péault, B., & Huard, J. (2014). Isolation of blood-vessel-derived multipotent precursors from human skeletal muscle. DOI: 10.3791/51195

Isolation of blood-vessel-derived multipotent precursors from human skeletal muscle. / Chen, William C W; Saparov, Arman; Corselli, Mirko; Crisan, Mihaela; Zheng, Bo; Péault, Bruno; Huard, Johnny.

In: Journal of Visualized Experiments, No. 90, 2014, p. e51195.

Research output: Contribution to journalArticle

Chen, WCW, Saparov, A, Corselli, M, Crisan, M, Zheng, B, Péault, B & Huard, J 2014, 'Isolation of blood-vessel-derived multipotent precursors from human skeletal muscle' Journal of Visualized Experiments, no. 90, pp. e51195. DOI: 10.3791/51195
Chen WCW, Saparov A, Corselli M, Crisan M, Zheng B, Péault B et al. Isolation of blood-vessel-derived multipotent precursors from human skeletal muscle. Journal of Visualized Experiments. 2014;(90):e51195. Available from, DOI: 10.3791/51195
Chen, William C W ; Saparov, Arman ; Corselli, Mirko ; Crisan, Mihaela ; Zheng, Bo ; Péault, Bruno ; Huard, Johnny. / Isolation of blood-vessel-derived multipotent precursors from human skeletal muscle. In: Journal of Visualized Experiments. 2014 ; No. 90. pp. e51195
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