Kinetics of Nitric Oxide Liberation by 3,4-Dihydro-1,2-Diazete 1,2-Dioxides and Their Vasodilatory Properties in Vitro and in Vivo

D. I. Utepbergenov, V. V. Khramtsov, L. P. Vlassenko, A. L. Markel, D. G. Mazhukin, A. Y. Tikhonov, L. B. Volodarsky

Research output: Contribution to journalArticle

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Abstract

Derivatives of 3,4-dihydro-1,2-diazete 1,2-dioxides (DD) have been investigated as NO donors in vitro and in vivo. Using nitronylnitroxides as spin traps for NO, these compounds were shown to decompose in water solutions al physiological pH and temperature, producing two molecules of NO per one DD molecule. Rate constants of DD decomposition were found to be in the range from 10-8 to 6.5×10-7 c(-1) in water and between 3×10-7 and 1.6×10-5 c-1 in dimethylsulfoxide. In vitro experiments performed with perfused rat tail artery showed that some of DD derivatives are highly effective vasodilators in concentrations from 5 to 80 μM while standard NO donor 3-(4-morpholino)-sydnonimine, SIN-1, does not lead to arterial vasodilatation in these concentrations. Significant (up to 30%) decrease of systolic arterial blood pressure was observed in hereditary hypertensive rats (ISIAH-strain) when some of DD were injected intraperitoneally in doses 40-200 μ/kg b.w., while the same effect of trinitroglycerin, TNG, was found at much higher dose equal to 900 μg/kg b.w.

Original languageEnglish
Pages (from-to)1023-1032
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume214
Issue number3
DOIs
Publication statusPublished - Sep 25 1995
Externally publishedYes

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Rats
Nitric Oxide
Derivatives
Morpholinos
Molecules
Kinetics
Water
Blood pressure
Dimethyl Sulfoxide
Vasodilator Agents
Vasodilation
Tail
Rate constants
Arterial Pressure
Arteries
Decomposition
Temperature
Experiments
In Vitro Techniques
linsidomine

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

Kinetics of Nitric Oxide Liberation by 3,4-Dihydro-1,2-Diazete 1,2-Dioxides and Their Vasodilatory Properties in Vitro and in Vivo. / Utepbergenov, D. I.; Khramtsov, V. V.; Vlassenko, L. P.; Markel, A. L.; Mazhukin, D. G.; Tikhonov, A. Y.; Volodarsky, L. B.

In: Biochemical and Biophysical Research Communications, Vol. 214, No. 3, 25.09.1995, p. 1023-1032.

Research output: Contribution to journalArticle

Utepbergenov, D. I. ; Khramtsov, V. V. ; Vlassenko, L. P. ; Markel, A. L. ; Mazhukin, D. G. ; Tikhonov, A. Y. ; Volodarsky, L. B. / Kinetics of Nitric Oxide Liberation by 3,4-Dihydro-1,2-Diazete 1,2-Dioxides and Their Vasodilatory Properties in Vitro and in Vivo. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 214, No. 3. pp. 1023-1032.
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