TY - GEN
T1 - Landscape of neutralizing assessment of monoclonal antibodies against dengue virus
AU - Sun, Jing
AU - Zhang, Guang Lan
AU - Olsen, Lars Rønn
AU - Reinherz, Ellis L.
AU - Brusic, Vladimir
PY - 2013
Y1 - 2013
N2 - The majority of antibody binding sites (B-cell epitopes) on antigens are discontinuous. The binding between antigen and antibody is specific, but in some cases, the antibody elicited by one antigen will show cross-reactivity against other antigens. We have developed a bioinformatics-based approach for the analysis of sequence variability of neutralizing antibody binding sites and the assessment of coverage by individual neutralizing antibodies. The antigenic analysis of functional sites on the envelope (E) protein from dengue virus has been used as a case study. The description of B-cell epitopes, measurement of epitope similarity among different strains, and estimation of antibody neutralizing coverage provide insights in antibody cross-reactivity. We have defined a generalized method for the analysis of cross-reactivity of neutralizing antibodies that is also applicable to the analysis of other pathogens. This method adds to the toolset available for the characterization and the design of broadly neutralizing vaccines.
AB - The majority of antibody binding sites (B-cell epitopes) on antigens are discontinuous. The binding between antigen and antibody is specific, but in some cases, the antibody elicited by one antigen will show cross-reactivity against other antigens. We have developed a bioinformatics-based approach for the analysis of sequence variability of neutralizing antibody binding sites and the assessment of coverage by individual neutralizing antibodies. The antigenic analysis of functional sites on the envelope (E) protein from dengue virus has been used as a case study. The description of B-cell epitopes, measurement of epitope similarity among different strains, and estimation of antibody neutralizing coverage provide insights in antibody cross-reactivity. We have defined a generalized method for the analysis of cross-reactivity of neutralizing antibodies that is also applicable to the analysis of other pathogens. This method adds to the toolset available for the characterization and the design of broadly neutralizing vaccines.
KW - B-cell epitope
KW - Cross-reactivity
KW - Dengue virus
KW - Discontinuous peptide
KW - Neutralizing antibody
UR - http://www.scopus.com/inward/record.url?scp=84888153551&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84888153551&partnerID=8YFLogxK
U2 - 10.1145/2506583.2512359
DO - 10.1145/2506583.2512359
M3 - Conference contribution
AN - SCOPUS:84888153551
SN - 9781450324342
T3 - 2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013
SP - 836
EP - 842
BT - 2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013
T2 - 2013 4th ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013
Y2 - 22 September 2013 through 25 September 2013
ER -