Landscape of neutralizing assessment of monoclonal antibodies against dengue virus

Jing Sun, Guang Lan Zhang, Lars Rønn Olsen, Ellis L. Reinherz, Vladimir Brusic

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

The majority of antibody binding sites (B-cell epitopes) on antigens are discontinuous. The binding between antigen and antibody is specific, but in some cases, the antibody elicited by one antigen will show cross-reactivity against other antigens. We have developed a bioinformatics-based approach for the analysis of sequence variability of neutralizing antibody binding sites and the assessment of coverage by individual neutralizing antibodies. The antigenic analysis of functional sites on the envelope (E) protein from dengue virus has been used as a case study. The description of B-cell epitopes, measurement of epitope similarity among different strains, and estimation of antibody neutralizing coverage provide insights in antibody cross-reactivity. We have defined a generalized method for the analysis of cross-reactivity of neutralizing antibodies that is also applicable to the analysis of other pathogens. This method adds to the toolset available for the characterization and the design of broadly neutralizing vaccines.

Original languageEnglish
Title of host publication2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013
Pages836-842
Number of pages7
DOIs
Publication statusPublished - 2013
Event2013 4th ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013 - Wshington, DC, United States
Duration: Sep 22 2013Sep 25 2013

Publication series

Name2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013

Other

Other2013 4th ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013
CountryUnited States
CityWshington, DC
Period9/22/139/25/13

Keywords

  • B-cell epitope
  • Cross-reactivity
  • Dengue virus
  • Discontinuous peptide
  • Neutralizing antibody

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Health Informatics

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  • Cite this

    Sun, J., Zhang, G. L., Olsen, L. R., Reinherz, E. L., & Brusic, V. (2013). Landscape of neutralizing assessment of monoclonal antibodies against dengue virus. In 2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013 (pp. 836-842). (2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013). https://doi.org/10.1145/2506583.2512359