Light acclimation of shade-tolerant and light-resistant Tradescantia species: induction of chlorophyll a fluorescence and P700 photooxidation, expression of PsbS and Lhcb1 proteins

Vladimir I. Mishanin, Boris V. Trubitsin, Michael A. Benkov, Andrei A. Minin, Alexander N. Tikhonov

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In this work, we have compared photosynthetic performance and expression of the PsbS and Lhcb1 proteins in two contrast ecotypes of Tradescantia species, T. fluminensis (shade-tolerant) and T.sillamontana (light-resistant), grown at two intensities of light: 50–125 μmol photons m−2 s−1 (low light, LL) and 875–1000 μmol photons m−2 s−1 (high light, HL). Using the EPR method for measuring the P700 content, we have found that LL-grown plants of both species have higher (by a factor of ≈1.7–1.8) contents of PSI per fresh weight unit as compared to HL-grown plants. Acclimation of plants to LL or HL irradiation also influences the Chl(a + b) level and expression of the PsbS and Lhcb1 proteins. Immunoblotting analysis showed that acclimation to HL stimulates (by a factor of ≈1.7–1.8) the level of PsbS related to the total number of P700 centers. In light-resistant species T.sillamontana, the ratio PsbS/P700 is about 2-times higher than in shade-tolerant species T.fluminensis grown under the same conditions. This should enhance the capacity of their leaves for protection against the light stress. In agreement with these observations, the capacity of leaves for NPQ induction was enhanced during plant acclimation to HL. Kinetic studies of P700 photooxidation and light-induced changes in the yield of Chl a fluorescence also revealed that the short-term regulation of electron transport processes in chloroplasts, which manifested themselves in the kinetics of (Formula presented.) induction and the rate of Chl a fluorescence quenching, occurred more rapidly in HL-grown plants than in LL-grown plants. Thus, both factors, enhanced expression of PsbS and more rapid response of the photosynthetic electron transport chain to dark-to-light transitions should increase the capacity of HL-grown plants for their resistance to rapid fluctuations of solar light.

Original languageEnglish
Pages (from-to)1-17
Number of pages17
JournalPhotosynthesis Research
DOIs
Publication statusAccepted/In press - Apr 1 2016
Externally publishedYes

Fingerprint

Tradescantia
photooxidation
Photooxidation
Acclimatization
acclimation
shade
Fluorescence
fluorescence
chlorophyll
Light
Proteins
proteins
chlorophyll a
Electron Transport
Photons
Tradescantia fluminensis

Keywords

  • Acclimation to low and high light
  • Chl fluorescence
  • EPR
  • Lhcb1
  • Photosynthesis
  • PsbS
  • Tradescantia

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Biochemistry

Cite this

Light acclimation of shade-tolerant and light-resistant Tradescantia species : induction of chlorophyll a fluorescence and P700 photooxidation, expression of PsbS and Lhcb1 proteins. / Mishanin, Vladimir I.; Trubitsin, Boris V.; Benkov, Michael A.; Minin, Andrei A.; Tikhonov, Alexander N.

In: Photosynthesis Research, 01.04.2016, p. 1-17.

Research output: Contribution to journalArticle

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abstract = "In this work, we have compared photosynthetic performance and expression of the PsbS and Lhcb1 proteins in two contrast ecotypes of Tradescantia species, T. fluminensis (shade-tolerant) and T.sillamontana (light-resistant), grown at two intensities of light: 50–125 μmol photons m−2 s−1 (low light, LL) and 875–1000 μmol photons m−2 s−1 (high light, HL). Using the EPR method for measuring the P700 content, we have found that LL-grown plants of both species have higher (by a factor of ≈1.7–1.8) contents of PSI per fresh weight unit as compared to HL-grown plants. Acclimation of plants to LL or HL irradiation also influences the Chl(a + b) level and expression of the PsbS and Lhcb1 proteins. Immunoblotting analysis showed that acclimation to HL stimulates (by a factor of ≈1.7–1.8) the level of PsbS related to the total number of P700 centers. In light-resistant species T.sillamontana, the ratio PsbS/P700 is about 2-times higher than in shade-tolerant species T.fluminensis grown under the same conditions. This should enhance the capacity of their leaves for protection against the light stress. In agreement with these observations, the capacity of leaves for NPQ induction was enhanced during plant acclimation to HL. Kinetic studies of P700 photooxidation and light-induced changes in the yield of Chl a fluorescence also revealed that the short-term regulation of electron transport processes in chloroplasts, which manifested themselves in the kinetics of (Formula presented.) induction and the rate of Chl a fluorescence quenching, occurred more rapidly in HL-grown plants than in LL-grown plants. Thus, both factors, enhanced expression of PsbS and more rapid response of the photosynthetic electron transport chain to dark-to-light transitions should increase the capacity of HL-grown plants for their resistance to rapid fluctuations of solar light.",
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AU - Trubitsin, Boris V.

AU - Benkov, Michael A.

AU - Minin, Andrei A.

AU - Tikhonov, Alexander N.

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AB - In this work, we have compared photosynthetic performance and expression of the PsbS and Lhcb1 proteins in two contrast ecotypes of Tradescantia species, T. fluminensis (shade-tolerant) and T.sillamontana (light-resistant), grown at two intensities of light: 50–125 μmol photons m−2 s−1 (low light, LL) and 875–1000 μmol photons m−2 s−1 (high light, HL). Using the EPR method for measuring the P700 content, we have found that LL-grown plants of both species have higher (by a factor of ≈1.7–1.8) contents of PSI per fresh weight unit as compared to HL-grown plants. Acclimation of plants to LL or HL irradiation also influences the Chl(a + b) level and expression of the PsbS and Lhcb1 proteins. Immunoblotting analysis showed that acclimation to HL stimulates (by a factor of ≈1.7–1.8) the level of PsbS related to the total number of P700 centers. In light-resistant species T.sillamontana, the ratio PsbS/P700 is about 2-times higher than in shade-tolerant species T.fluminensis grown under the same conditions. This should enhance the capacity of their leaves for protection against the light stress. In agreement with these observations, the capacity of leaves for NPQ induction was enhanced during plant acclimation to HL. Kinetic studies of P700 photooxidation and light-induced changes in the yield of Chl a fluorescence also revealed that the short-term regulation of electron transport processes in chloroplasts, which manifested themselves in the kinetics of (Formula presented.) induction and the rate of Chl a fluorescence quenching, occurred more rapidly in HL-grown plants than in LL-grown plants. Thus, both factors, enhanced expression of PsbS and more rapid response of the photosynthetic electron transport chain to dark-to-light transitions should increase the capacity of HL-grown plants for their resistance to rapid fluctuations of solar light.

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