Abstract
We present a method for immunohistochemical demonstration of surface-associated thyroid-stimulating hormone (TSH) receptors on direct imprints of rat thyroid follicular cells at the light microscopic level, by use of dinitrophenyl (DNP)-labeled bovine TSH as the primary probe in a DNA-hapten sandwich staining (DHSS) procedure. The light microscopically invisible diaminobenzidine (DAB) product of the enzyme reaction was amplified with a DAB enhancement technique (silver amplification), which yielded a reliable and distinct light microscopically visible cell surface staining. The consistently negative control results after inhibition with TSH receptor-specific reagents provide evidence in support of immunohistochemical localization of surface membrane-associated TSH receptors.
Original language | English |
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Pages (from-to) | 977-982 |
Number of pages | 6 |
Journal | Journal of Histochemistry and Cytochemistry |
Volume | 36 |
Issue number | 8 |
DOIs | |
Publication status | Published - Jan 1 1988 |
ASJC Scopus subject areas
- Anatomy
- Histology