Light microscopic immunocytochemical demonstration of thryoid-stimulating hormone (TSH) receptors on normal rat thyroid cells

K. W. Schmid, B. Jasani, J. M. Morgan, E. D. Williams

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We present a method for immunohistochemical demonstration of surface-associated thyroid-stimulating hormone (TSH) receptors on direct imprints of rat thyroid follicular cells at the light microscopic level, by use of dinitrophenyl (DNP)-labeled bovine TSH as the primary probe in a DNA-hapten sandwich staining (DHSS) procedure. The light microscopically invisible diaminobenzidine (DAB) product of the enzyme reaction was amplified with a DAB enhancement technique (silver amplification), which yielded a reliable and distinct light microscopically visible cell surface staining. The consistently negative control results after inhibition with TSH receptor-specific reagents provide evidence in support of immunohistochemical localization of surface membrane-associated TSH receptors.

Original languageEnglish
Pages (from-to)977-982
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume36
Issue number8
DOIs
Publication statusPublished - Jan 1 1988

ASJC Scopus subject areas

  • Anatomy
  • Histology

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