Abstract
We present a method for immunohistochemical demonstration of surface-associated thyroid-stimulating hormone (TSH) receptors on direct imprints of rat thyroid follicular cells at the light microscopic level, by use of dinitrophenyl (DNP)-labeled bovine TSH as the primary probe in a DNA-hapten sandwich staining (DHSS) procedure. The light microscopically invisible diaminobenzidine (DAB) product of the enzyme reaction was amplified with a DAB enhancement technique (silver amplification), which yielded a reliable and distinct light microscopically visible cell surface staining. The consistently negative control results after inhibition with TSH receptor-specific reagents provide evidence in support of immunohistochemical localization of surface membrane-associated TSH receptors.
| Original language | English |
|---|---|
| Pages (from-to) | 977-982 |
| Number of pages | 6 |
| Journal | Journal of Histochemistry and Cytochemistry |
| Volume | 36 |
| Issue number | 8 |
| Publication status | Published - 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Anatomy
Cite this
Light microscopic immunocytochemical demonstration of thryoid-stimulating hormone (TSH) receptors on normal rat thyroid cells. / Schmid, K. W.; Jasani, B.; Morgan, J. M.; Williams, E. D.
In: Journal of Histochemistry and Cytochemistry, Vol. 36, No. 8, 1988, p. 977-982.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Light microscopic immunocytochemical demonstration of thryoid-stimulating hormone (TSH) receptors on normal rat thyroid cells
AU - Schmid, K. W.
AU - Jasani, B.
AU - Morgan, J. M.
AU - Williams, E. D.
PY - 1988
Y1 - 1988
N2 - We present a method for immunohistochemical demonstration of surface-associated thyroid-stimulating hormone (TSH) receptors on direct imprints of rat thyroid follicular cells at the light microscopic level, by use of dinitrophenyl (DNP)-labeled bovine TSH as the primary probe in a DNA-hapten sandwich staining (DHSS) procedure. The light microscopically invisible diaminobenzidine (DAB) product of the enzyme reaction was amplified with a DAB enhancement technique (silver amplification), which yielded a reliable and distinct light microscopically visible cell surface staining. The consistently negative control results after inhibition with TSH receptor-specific reagents provide evidence in support of immunohistochemical localization of surface membrane-associated TSH receptors.
AB - We present a method for immunohistochemical demonstration of surface-associated thyroid-stimulating hormone (TSH) receptors on direct imprints of rat thyroid follicular cells at the light microscopic level, by use of dinitrophenyl (DNP)-labeled bovine TSH as the primary probe in a DNA-hapten sandwich staining (DHSS) procedure. The light microscopically invisible diaminobenzidine (DAB) product of the enzyme reaction was amplified with a DAB enhancement technique (silver amplification), which yielded a reliable and distinct light microscopically visible cell surface staining. The consistently negative control results after inhibition with TSH receptor-specific reagents provide evidence in support of immunohistochemical localization of surface membrane-associated TSH receptors.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023740291&partnerID=8YFLogxK
M3 - Article
VL - 36
SP - 977
EP - 982
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
SN - 0022-1554
IS - 8
ER -