Light microscopic immunocytochemical demonstration of thryoid-stimulating hormone (TSH) receptors on normal rat thyroid cells

K. W. Schmid, B. Jasani, J. M. Morgan, E. D. Williams

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We present a method for immunohistochemical demonstration of surface-associated thyroid-stimulating hormone (TSH) receptors on direct imprints of rat thyroid follicular cells at the light microscopic level, by use of dinitrophenyl (DNP)-labeled bovine TSH as the primary probe in a DNA-hapten sandwich staining (DHSS) procedure. The light microscopically invisible diaminobenzidine (DAB) product of the enzyme reaction was amplified with a DAB enhancement technique (silver amplification), which yielded a reliable and distinct light microscopically visible cell surface staining. The consistently negative control results after inhibition with TSH receptor-specific reagents provide evidence in support of immunohistochemical localization of surface membrane-associated TSH receptors.

Original languageEnglish
Pages (from-to)977-982
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume36
Issue number8
Publication statusPublished - 1988
Externally publishedYes

Fingerprint

Thyroid Gland
Hormones
Light
Staining and Labeling
Thyrotropin Receptors
Haptens
Silver
Membranes
DNA
Enzymes
Thyroid Epithelial Cells

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Light microscopic immunocytochemical demonstration of thryoid-stimulating hormone (TSH) receptors on normal rat thyroid cells. / Schmid, K. W.; Jasani, B.; Morgan, J. M.; Williams, E. D.

In: Journal of Histochemistry and Cytochemistry, Vol. 36, No. 8, 1988, p. 977-982.

Research output: Contribution to journalArticle

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