Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress

Pradeep K  Papolu; Muthusamy Ramakrishnan; Qiang  Wei; Kunnummal Kurungara Vinod; Kim Yrjälä; Ruslan Kalendar; Mingbing Zhou

doi:10.1186/s12870-021-03339-1

Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress

Pradeep K Papolu, Muthusamy Ramakrishnan, Qiang Wei , Kunnummal Kurungara Vinod, Kim Yrjälä, Ruslan Kalendar, Mingbing Zhou

Laboratory of bioinformatics and systems biology

Research output: Contribution to journal › Article › peer-review

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Abstract

LTR retrotransposons play a significant role in plant growth, genome evolution, and environmental stress response, but their regulatory response to heat stress remains unclear. We have investigated the activities of two LTR retrotransposons, PHRE1 and PHRE2, of moso bamboo (Phyllostachys edulis) in response to heat stress.
The differential overexpression of PHRE1 and PHRE2 with or without CaMV35s promoter showed enhanced expression under heat stress in transgenic plants. The transcriptional activity studies showed an increase in transposition activity and copy number among moso bamboo wild type and Arabidopsis transgenic plants under heat stress. Comparison of promoter activity in transgenic plants indicated that 5’LTR promoter activity was higher than CaMV35s promoter. Additionally, yeast one-hybrid (Y1H) system and in planta biomolecular fluorescence complementation (BiFC) assay revealed interactions of heat-dependent transcription factors (TFs) with 5’LTR sequence and direct interactions of TFs with pol and gag.
Our results conclude that the 5’LTR acts as a promoter and could regulate the LTR retrotransposons in moso bamboo under heat stress.

Original language	English
Article number	585
Journal	BMC Plant Biology
Volume	21
DOIs	https://doi.org/10.1186/s12870-021-03339-1
Publication status	Published - Dec 9 2021

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title = "Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress",

abstract = "LTR retrotransposons play a significant role in plant growth, genome evolution, and environmental stress response, but their regulatory response to heat stress remains unclear. We have investigated the activities of two LTR retrotransposons, PHRE1 and PHRE2, of moso bamboo (Phyllostachys edulis) in response to heat stress.The differential overexpression of PHRE1 and PHRE2 with or without CaMV35s promoter showed enhanced expression under heat stress in transgenic plants. The transcriptional activity studies showed an increase in transposition activity and copy number among moso bamboo wild type and Arabidopsis transgenic plants under heat stress. Comparison of promoter activity in transgenic plants indicated that 5{\textquoteright}LTR promoter activity was higher than CaMV35s promoter. Additionally, yeast one-hybrid (Y1H) system and in planta biomolecular fluorescence complementation (BiFC) assay revealed interactions of heat-dependent transcription factors (TFs) with 5{\textquoteright}LTR sequence and direct interactions of TFs with pol and gag.Our results conclude that the 5{\textquoteright}LTR acts as a promoter and could regulate the LTR retrotransposons in moso bamboo under heat stress.",

author = "Papolu, {Pradeep K} and Muthusamy Ramakrishnan and Qiang Wei and Vinod, {Kunnummal Kurungara} and Kim Yrj{\"a}l{\"a} and Ruslan Kalendar and Mingbing Zhou",

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doi = "10.1186/s12870-021-03339-1",

language = "English",

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journal = "BMC Plant Biology",

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TY - JOUR

T1 - Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress

AU - Papolu, Pradeep K

AU - Ramakrishnan, Muthusamy

AU - Wei , Qiang

AU - Vinod, Kunnummal Kurungara

AU - Yrjälä, Kim

AU - Kalendar, Ruslan

AU - Zhou, Mingbing

PY - 2021/12/9

Y1 - 2021/12/9

N2 - LTR retrotransposons play a significant role in plant growth, genome evolution, and environmental stress response, but their regulatory response to heat stress remains unclear. We have investigated the activities of two LTR retrotransposons, PHRE1 and PHRE2, of moso bamboo (Phyllostachys edulis) in response to heat stress.The differential overexpression of PHRE1 and PHRE2 with or without CaMV35s promoter showed enhanced expression under heat stress in transgenic plants. The transcriptional activity studies showed an increase in transposition activity and copy number among moso bamboo wild type and Arabidopsis transgenic plants under heat stress. Comparison of promoter activity in transgenic plants indicated that 5’LTR promoter activity was higher than CaMV35s promoter. Additionally, yeast one-hybrid (Y1H) system and in planta biomolecular fluorescence complementation (BiFC) assay revealed interactions of heat-dependent transcription factors (TFs) with 5’LTR sequence and direct interactions of TFs with pol and gag.Our results conclude that the 5’LTR acts as a promoter and could regulate the LTR retrotransposons in moso bamboo under heat stress.

AB - LTR retrotransposons play a significant role in plant growth, genome evolution, and environmental stress response, but their regulatory response to heat stress remains unclear. We have investigated the activities of two LTR retrotransposons, PHRE1 and PHRE2, of moso bamboo (Phyllostachys edulis) in response to heat stress.The differential overexpression of PHRE1 and PHRE2 with or without CaMV35s promoter showed enhanced expression under heat stress in transgenic plants. The transcriptional activity studies showed an increase in transposition activity and copy number among moso bamboo wild type and Arabidopsis transgenic plants under heat stress. Comparison of promoter activity in transgenic plants indicated that 5’LTR promoter activity was higher than CaMV35s promoter. Additionally, yeast one-hybrid (Y1H) system and in planta biomolecular fluorescence complementation (BiFC) assay revealed interactions of heat-dependent transcription factors (TFs) with 5’LTR sequence and direct interactions of TFs with pol and gag.Our results conclude that the 5’LTR acts as a promoter and could regulate the LTR retrotransposons in moso bamboo under heat stress.

U2 - 10.1186/s12870-021-03339-1

DO - 10.1186/s12870-021-03339-1

M3 - Article

SN - 1471-2229

VL - 21

JO - BMC Plant Biology

JF - BMC Plant Biology

M1 - 585

ER -

Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress

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