Loss of membranous E-cadherin expression in pancreatic cancer

correlation with lymph node metastasis, high grade, and advanced stage.

M. Pignatelli, T. W. Ansari, P. Gunter, D. Liu, S. Hirano, M. Takeichi, G. Klöppel, N. R. Lemoine

Research output: Contribution to journalArticle

213 Citations (Scopus)

Abstract

Epithelial cadherin (E-cadherin) is a Ca(2+)-dependent cell-cell adhesion molecule that connects cells via homotypic interactions. Its function is critical in the induction and maintenance of cell polarity and differentiation, and its loss of downregulation is associated with an invasive and poorly differentiated phenotype in colon and other tumors. We have used an avidin-biotin immunoperoxidase technique to localize E-cadherin in microwave-treated, paraffin-embedded sections from 36 patients with pancreatic adenocarcinomas. E-cadherin was expressed by normal ductal and acinar cells with typical membranous staining at the intercellular junctions. Loss of normal surface E-cadherin expression was found in 19/36 (53 per cent) tumours compared to the adjacent normal ductal cells. Abnormal E-cadherin expression was found more frequently in poorly differentiated (grade III) (6/7, 86 per cent) than in well-differentiated tumors (grade I) (4/14, 28 per cent) (P = 0.012). Membranous E-cadherin expression was also lost more frequently in primary tumours with lymph node (stage III) (14/23, 61 per cent) and distant metastasis (stage IV) (2/2, 100 per cent) compared with 3/11 (27 per cent) lymph node-negative tumours (stage I) (P = 0.043). In conclusions, our data indicate that loss of membranous E-cadherin expression is associated with high grade and advanced stage in pancreatic cancer.

Original languageEnglish
Pages (from-to)243-248
Number of pages6
JournalJournal of Pathology
Volume174
Issue number4
Publication statusPublished - Dec 1994
Externally publishedYes

Fingerprint

Cadherins
Pancreatic Neoplasms
Lymph Nodes
Neoplasm Metastasis
Neoplasms
Cell Polarity
Intercellular Junctions
Avidin
Acinar Cells
Cell Adhesion Molecules
Biotin
Microwaves
Immunoenzyme Techniques
Paraffin
Cell Differentiation
Colon
Adenocarcinoma
Down-Regulation
Maintenance
Staining and Labeling

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Loss of membranous E-cadherin expression in pancreatic cancer : correlation with lymph node metastasis, high grade, and advanced stage. / Pignatelli, M.; Ansari, T. W.; Gunter, P.; Liu, D.; Hirano, S.; Takeichi, M.; Klöppel, G.; Lemoine, N. R.

In: Journal of Pathology, Vol. 174, No. 4, 12.1994, p. 243-248.

Research output: Contribution to journalArticle

Pignatelli, M, Ansari, TW, Gunter, P, Liu, D, Hirano, S, Takeichi, M, Klöppel, G & Lemoine, NR 1994, 'Loss of membranous E-cadherin expression in pancreatic cancer: correlation with lymph node metastasis, high grade, and advanced stage.', Journal of Pathology, vol. 174, no. 4, pp. 243-248.
Pignatelli, M. ; Ansari, T. W. ; Gunter, P. ; Liu, D. ; Hirano, S. ; Takeichi, M. ; Klöppel, G. ; Lemoine, N. R. / Loss of membranous E-cadherin expression in pancreatic cancer : correlation with lymph node metastasis, high grade, and advanced stage. In: Journal of Pathology. 1994 ; Vol. 174, No. 4. pp. 243-248.
@article{08de553c779e4610948830f8efc9d474,
title = "Loss of membranous E-cadherin expression in pancreatic cancer: correlation with lymph node metastasis, high grade, and advanced stage.",
abstract = "Epithelial cadherin (E-cadherin) is a Ca(2+)-dependent cell-cell adhesion molecule that connects cells via homotypic interactions. Its function is critical in the induction and maintenance of cell polarity and differentiation, and its loss of downregulation is associated with an invasive and poorly differentiated phenotype in colon and other tumors. We have used an avidin-biotin immunoperoxidase technique to localize E-cadherin in microwave-treated, paraffin-embedded sections from 36 patients with pancreatic adenocarcinomas. E-cadherin was expressed by normal ductal and acinar cells with typical membranous staining at the intercellular junctions. Loss of normal surface E-cadherin expression was found in 19/36 (53 per cent) tumours compared to the adjacent normal ductal cells. Abnormal E-cadherin expression was found more frequently in poorly differentiated (grade III) (6/7, 86 per cent) than in well-differentiated tumors (grade I) (4/14, 28 per cent) (P = 0.012). Membranous E-cadherin expression was also lost more frequently in primary tumours with lymph node (stage III) (14/23, 61 per cent) and distant metastasis (stage IV) (2/2, 100 per cent) compared with 3/11 (27 per cent) lymph node-negative tumours (stage I) (P = 0.043). In conclusions, our data indicate that loss of membranous E-cadherin expression is associated with high grade and advanced stage in pancreatic cancer.",
author = "M. Pignatelli and Ansari, {T. W.} and P. Gunter and D. Liu and S. Hirano and M. Takeichi and G. Kl{\"o}ppel and Lemoine, {N. R.}",
year = "1994",
month = "12",
language = "English",
volume = "174",
pages = "243--248",
journal = "Journal of Pathology",
issn = "0022-3417",
publisher = "John Wiley and Sons Ltd",
number = "4",

}

TY - JOUR

T1 - Loss of membranous E-cadherin expression in pancreatic cancer

T2 - correlation with lymph node metastasis, high grade, and advanced stage.

AU - Pignatelli, M.

AU - Ansari, T. W.

AU - Gunter, P.

AU - Liu, D.

AU - Hirano, S.

AU - Takeichi, M.

AU - Klöppel, G.

AU - Lemoine, N. R.

PY - 1994/12

Y1 - 1994/12

N2 - Epithelial cadherin (E-cadherin) is a Ca(2+)-dependent cell-cell adhesion molecule that connects cells via homotypic interactions. Its function is critical in the induction and maintenance of cell polarity and differentiation, and its loss of downregulation is associated with an invasive and poorly differentiated phenotype in colon and other tumors. We have used an avidin-biotin immunoperoxidase technique to localize E-cadherin in microwave-treated, paraffin-embedded sections from 36 patients with pancreatic adenocarcinomas. E-cadherin was expressed by normal ductal and acinar cells with typical membranous staining at the intercellular junctions. Loss of normal surface E-cadherin expression was found in 19/36 (53 per cent) tumours compared to the adjacent normal ductal cells. Abnormal E-cadherin expression was found more frequently in poorly differentiated (grade III) (6/7, 86 per cent) than in well-differentiated tumors (grade I) (4/14, 28 per cent) (P = 0.012). Membranous E-cadherin expression was also lost more frequently in primary tumours with lymph node (stage III) (14/23, 61 per cent) and distant metastasis (stage IV) (2/2, 100 per cent) compared with 3/11 (27 per cent) lymph node-negative tumours (stage I) (P = 0.043). In conclusions, our data indicate that loss of membranous E-cadherin expression is associated with high grade and advanced stage in pancreatic cancer.

AB - Epithelial cadherin (E-cadherin) is a Ca(2+)-dependent cell-cell adhesion molecule that connects cells via homotypic interactions. Its function is critical in the induction and maintenance of cell polarity and differentiation, and its loss of downregulation is associated with an invasive and poorly differentiated phenotype in colon and other tumors. We have used an avidin-biotin immunoperoxidase technique to localize E-cadherin in microwave-treated, paraffin-embedded sections from 36 patients with pancreatic adenocarcinomas. E-cadherin was expressed by normal ductal and acinar cells with typical membranous staining at the intercellular junctions. Loss of normal surface E-cadherin expression was found in 19/36 (53 per cent) tumours compared to the adjacent normal ductal cells. Abnormal E-cadherin expression was found more frequently in poorly differentiated (grade III) (6/7, 86 per cent) than in well-differentiated tumors (grade I) (4/14, 28 per cent) (P = 0.012). Membranous E-cadherin expression was also lost more frequently in primary tumours with lymph node (stage III) (14/23, 61 per cent) and distant metastasis (stage IV) (2/2, 100 per cent) compared with 3/11 (27 per cent) lymph node-negative tumours (stage I) (P = 0.043). In conclusions, our data indicate that loss of membranous E-cadherin expression is associated with high grade and advanced stage in pancreatic cancer.

UR - http://www.scopus.com/inward/record.url?scp=0028691011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028691011&partnerID=8YFLogxK

M3 - Article

VL - 174

SP - 243

EP - 248

JO - Journal of Pathology

JF - Journal of Pathology

SN - 0022-3417

IS - 4

ER -