Lysozyme gene expression in inflammatory bowel disease

G. W H Stamp, R. Poulsom, L. P. Chung, S. Keshav, R. E. Jeffery, J. A. Longcroft, M. Pignatelli, N. A. Wright

Research output: Contribution to journalArticle

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Abstract

Riboprobe in situ hybridization (rISH) demonstrates active lysozyme synthesis in ulcerative colitis and Crohn's disease. Maximal labeling was seen in Paneth cells, macrophages, and granulomas. Diffuse infiltration of the mucosa by lysozyme-rich polymorphs characterizes ulcerative colitis but obscures reactivity in other cell lineages in immunohistochemical studies; lysozyme mRNA is not detected in polymorphs, rISH giving a clearer picture than immunohistochemical studies of the active synthesis of lysozyme within the gut in inflammatory bowel disease. In ulcerative colitis, strong signals localized to Paneth cell metaplasia were found in 11 of 20 cases and to a lesser degree in non-Paneth cell lineages in regenerative mucosa in 13 of 20 cases. In Crohn's disease, abundant labeling was seen in tuberculoid granulomas (5 of 20) and over macrophage aggregates in the lamina propria in another 7, characteristic patterns not encountered in ulcerative colitis. Low levels of lysozyme messenger RNA were found in the ulceration-associated cell lineage ("pseudopyloric metaplasia"). These results support the view that neutrophils are largely responsible for elevated fecal lysozyme levels in ulcerative colitis and macrophages for elevated serum lysozyme levels in Crohn's disease.

Original languageEnglish
Pages (from-to)532-538
Number of pages7
JournalGastroenterology
Volume103
Issue number2
Publication statusPublished - 1992
Externally publishedYes

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Muramidase
Inflammatory Bowel Diseases
Ulcerative Colitis
Gene Expression
Cell Lineage
Crohn Disease
Paneth Cells
Mucous Membrane
Macrophages
Metaplasia
Granuloma
In Situ Hybridization
Messenger RNA
Neutrophils
Serum

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Stamp, G. W. H., Poulsom, R., Chung, L. P., Keshav, S., Jeffery, R. E., Longcroft, J. A., ... Wright, N. A. (1992). Lysozyme gene expression in inflammatory bowel disease. Gastroenterology, 103(2), 532-538.

Lysozyme gene expression in inflammatory bowel disease. / Stamp, G. W H; Poulsom, R.; Chung, L. P.; Keshav, S.; Jeffery, R. E.; Longcroft, J. A.; Pignatelli, M.; Wright, N. A.

In: Gastroenterology, Vol. 103, No. 2, 1992, p. 532-538.

Research output: Contribution to journalArticle

Stamp, GWH, Poulsom, R, Chung, LP, Keshav, S, Jeffery, RE, Longcroft, JA, Pignatelli, M & Wright, NA 1992, 'Lysozyme gene expression in inflammatory bowel disease', Gastroenterology, vol. 103, no. 2, pp. 532-538.
Stamp GWH, Poulsom R, Chung LP, Keshav S, Jeffery RE, Longcroft JA et al. Lysozyme gene expression in inflammatory bowel disease. Gastroenterology. 1992;103(2):532-538.
Stamp, G. W H ; Poulsom, R. ; Chung, L. P. ; Keshav, S. ; Jeffery, R. E. ; Longcroft, J. A. ; Pignatelli, M. ; Wright, N. A. / Lysozyme gene expression in inflammatory bowel disease. In: Gastroenterology. 1992 ; Vol. 103, No. 2. pp. 532-538.
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