M31 and macroH2A1.2 colocalise at the pseudoautosomal region during mouse meiosis

J M Turner, P S Burgoyne, P B Singh

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Progression through meiotic prophase is associated with dramatic changes in chromosome condensation. Two proteins that have been implicated in effecting these changes are the mammalian HP1-like protein M31 (HP1beta or MOD1) and the unusual core histone macroH2A1.2. Previous analyses of M31 and macroH2A1.2 localisation in mouse testis sections have indicated that both proteins are components of meiotic centromeric heterochromatin and of the sex body, the transcriptionally inactive domain of the X and Y chromosomes. This second observation has raised the possibility that these proteins co-operate in meiotic sex chromosome inactivation. In order to investigate the roles of M31 and macroH2A1.2 in meiosis in greater detail, we have examined their localisation patterns in surface-spread meiocytes from male and female mice. Using this approach, we report that, in addition to their previous described staining patterns, both proteins localise to a focus within the portion of the pseudoautosomal region (PAR) that contains the steroid sulphatase (Sts) gene. In light of the timing of its appearance and of its behaviour in sex-chromosomally variant mice, we suggest a role for this heterochromatin focus in preventing complete desynapsis of the terminally associated X and Y chromosomes prior to anaphase I.

Original languageEnglish
Pages (from-to)3367-75
Number of pages9
JournalJournal of Cell Science
Volume114
Issue numberPt 18
Publication statusPublished - Sep 2001

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Meiosis
Heterochromatin
Y Chromosome
X Chromosome
Proteins
Steryl-Sulfatase
Prophase
Anaphase
Sex Chromosomes
Sexual Behavior
Histones
Testis
Chromosomes
Observation
macroH2A histone
Pseudoautosomal Regions
Staining and Labeling
Genes

Keywords

  • Animals
  • Arylsulfatases
  • Chromosomal Proteins, Non-Histone
  • Chromosome Pairing
  • Female
  • Heterochromatin
  • Histones
  • Male
  • Meiosis
  • Mice
  • Oocytes
  • Physical Chromosome Mapping
  • Prophase
  • Sex Chromosomes
  • Spermatozoa
  • Steryl-Sulfatase
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

M31 and macroH2A1.2 colocalise at the pseudoautosomal region during mouse meiosis. / Turner, J M; Burgoyne, P S; Singh, P B.

In: Journal of Cell Science, Vol. 114, No. Pt 18, 09.2001, p. 3367-75.

Research output: Contribution to journalArticle

Turner, J M ; Burgoyne, P S ; Singh, P B. / M31 and macroH2A1.2 colocalise at the pseudoautosomal region during mouse meiosis. In: Journal of Cell Science. 2001 ; Vol. 114, No. Pt 18. pp. 3367-75.
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AB - Progression through meiotic prophase is associated with dramatic changes in chromosome condensation. Two proteins that have been implicated in effecting these changes are the mammalian HP1-like protein M31 (HP1beta or MOD1) and the unusual core histone macroH2A1.2. Previous analyses of M31 and macroH2A1.2 localisation in mouse testis sections have indicated that both proteins are components of meiotic centromeric heterochromatin and of the sex body, the transcriptionally inactive domain of the X and Y chromosomes. This second observation has raised the possibility that these proteins co-operate in meiotic sex chromosome inactivation. In order to investigate the roles of M31 and macroH2A1.2 in meiosis in greater detail, we have examined their localisation patterns in surface-spread meiocytes from male and female mice. Using this approach, we report that, in addition to their previous described staining patterns, both proteins localise to a focus within the portion of the pseudoautosomal region (PAR) that contains the steroid sulphatase (Sts) gene. In light of the timing of its appearance and of its behaviour in sex-chromosomally variant mice, we suggest a role for this heterochromatin focus in preventing complete desynapsis of the terminally associated X and Y chromosomes prior to anaphase I.

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KW - Male

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