M32, a murine homologue of Drosophila heterochromatin protein 1 (HP1), localises to euchromatin within interphase nuclei and is largely excluded from constitutive heterochromatin

D Horsley, A Hutchings, G W Butcher, P B Singh

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Mice possess two structural homologues of Drosophila HP1, termed M31 and M32 (Singh et al., 1991). We have previously shown that an M31-specific monoclonal antibody (MoAb), MAC 353, localises to constitutive heterochromatin (Wreggett et al., 1994). Here we report that a MoAb raised against the M32 protein (MAC 385) recognises a 22-kDa protein in murine nuclear extracts and that M32 is distributed in a fine-grain "speckled" pattern within interphase nuclei. M32 is also largely excluded from the large masses of constitutive heterochromatin that are labelled by MAC 353.

Original languageEnglish
Pages (from-to)308-11
Number of pages4
JournalCytogenetic and Genome Research
Volume73
Issue number4
Publication statusPublished - 1996

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Cell Line
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • Drosophila melanogaster
  • Euchromatin
  • Evolution, Molecular
  • Genes
  • Genes, Insect
  • Heterochromatin
  • Interphase
  • Mice
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Comparative Study
  • Journal Article

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