M32, a murine homologue of Drosophila heterochromatin protein 1 (HP1), localises to euchromatin within interphase nuclei and is largely excluded from constitutive heterochromatin

D Horsley, A Hutchings, G W Butcher, P B Singh

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Abstract

Mice possess two structural homologues of Drosophila HP1, termed M31 and M32 (Singh et al., 1991). We have previously shown that an M31-specific monoclonal antibody (MoAb), MAC 353, localises to constitutive heterochromatin (Wreggett et al., 1994). Here we report that a MoAb raised against the M32 protein (MAC 385) recognises a 22-kDa protein in murine nuclear extracts and that M32 is distributed in a fine-grain "speckled" pattern within interphase nuclei. M32 is also largely excluded from the large masses of constitutive heterochromatin that are labelled by MAC 353.

Original languageEnglish
Pages (from-to)308-11
Number of pages4
JournalCytogenetic and Genome Research
Volume73
Issue number4
Publication statusPublished - 1996

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Euchromatin
Heterochromatin
Interphase
Monoclonal Antibodies
Proteins
Drosophila heterochromatin protein 1

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Cell Line
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • Drosophila melanogaster
  • Euchromatin
  • Evolution, Molecular
  • Genes
  • Genes, Insect
  • Heterochromatin
  • Interphase
  • Mice
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Comparative Study
  • Journal Article

Cite this

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title = "M32, a murine homologue of Drosophila heterochromatin protein 1 (HP1), localises to euchromatin within interphase nuclei and is largely excluded from constitutive heterochromatin",
abstract = "Mice possess two structural homologues of Drosophila HP1, termed M31 and M32 (Singh et al., 1991). We have previously shown that an M31-specific monoclonal antibody (MoAb), MAC 353, localises to constitutive heterochromatin (Wreggett et al., 1994). Here we report that a MoAb raised against the M32 protein (MAC 385) recognises a 22-kDa protein in murine nuclear extracts and that M32 is distributed in a fine-grain {"}speckled{"} pattern within interphase nuclei. M32 is also largely excluded from the large masses of constitutive heterochromatin that are labelled by MAC 353.",
keywords = "Animals, Antibodies, Monoclonal, Cell Line, Chromatin, Chromosomal Proteins, Non-Histone, Drosophila melanogaster, Euchromatin, Evolution, Molecular, Genes, Genes, Insect, Heterochromatin, Interphase, Mice, Sequence Homology, Amino Acid, Species Specificity, Comparative Study, Journal Article",
author = "D Horsley and A Hutchings and Butcher, {G W} and Singh, {P B}",
year = "1996",
language = "English",
volume = "73",
pages = "308--11",
journal = "Cytogenetic and Genome Research",
issn = "1424-8581",
publisher = "S. Karger AG",
number = "4",

}

TY - JOUR

T1 - M32, a murine homologue of Drosophila heterochromatin protein 1 (HP1), localises to euchromatin within interphase nuclei and is largely excluded from constitutive heterochromatin

AU - Horsley, D

AU - Hutchings, A

AU - Butcher, G W

AU - Singh, P B

PY - 1996

Y1 - 1996

N2 - Mice possess two structural homologues of Drosophila HP1, termed M31 and M32 (Singh et al., 1991). We have previously shown that an M31-specific monoclonal antibody (MoAb), MAC 353, localises to constitutive heterochromatin (Wreggett et al., 1994). Here we report that a MoAb raised against the M32 protein (MAC 385) recognises a 22-kDa protein in murine nuclear extracts and that M32 is distributed in a fine-grain "speckled" pattern within interphase nuclei. M32 is also largely excluded from the large masses of constitutive heterochromatin that are labelled by MAC 353.

AB - Mice possess two structural homologues of Drosophila HP1, termed M31 and M32 (Singh et al., 1991). We have previously shown that an M31-specific monoclonal antibody (MoAb), MAC 353, localises to constitutive heterochromatin (Wreggett et al., 1994). Here we report that a MoAb raised against the M32 protein (MAC 385) recognises a 22-kDa protein in murine nuclear extracts and that M32 is distributed in a fine-grain "speckled" pattern within interphase nuclei. M32 is also largely excluded from the large masses of constitutive heterochromatin that are labelled by MAC 353.

KW - Animals

KW - Antibodies, Monoclonal

KW - Cell Line

KW - Chromatin

KW - Chromosomal Proteins, Non-Histone

KW - Drosophila melanogaster

KW - Euchromatin

KW - Evolution, Molecular

KW - Genes

KW - Genes, Insect

KW - Heterochromatin

KW - Interphase

KW - Mice

KW - Sequence Homology, Amino Acid

KW - Species Specificity

KW - Comparative Study

KW - Journal Article

M3 - Article

VL - 73

SP - 308

EP - 311

JO - Cytogenetic and Genome Research

JF - Cytogenetic and Genome Research

SN - 1424-8581

IS - 4

ER -