Maintenance of stable heterochromatin domains by dynamic HP1 binding

Thierry Cheutin, Adrian J McNairn, Thomas Jenuwein, David M Gilbert, Prim B Singh, Tom Misteli

Research output: Contribution to journalArticle

428 Citations (Scopus)

Abstract

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and thus is a candidate for establishing and maintaining the transcriptionally repressive heterochromatin structure. Here we demonstrate that maintenance of stable heterochromatin domains in living cells involves the transient binding and dynamic exchange of HP1 from chromatin. HP1 exchange kinetics correlate with the condensation level of chromatin and are dependent on the histone methyltransferase Suv39h. The chromodomain and the chromoshadow domain of HP1 are both required for binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin. These data argue against HP1 repression of transcription by formation of static, higher order oligomeric networks but support a dynamic competition model, and they demonstrate that heterochromatin is accessible to regulatory factors.

Original languageEnglish
Pages (from-to)721-5
Number of pages5
JournalScience
Volume299
Issue number5607
DOIs
Publication statusPublished - Jan 31 2003

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Heterochromatin
Protein Binding
Maintenance
Chromatin
Euchromatin
Gene Silencing
Epigenomics
heterochromatin-specific nonhistone chromosomal protein HP-1

Keywords

  • Amanitins
  • Animals
  • Binding Sites
  • CHO Cells
  • Cell Nucleus
  • Cells, Cultured
  • Chromosomal Proteins, Non-Histone
  • Cricetinae
  • Dimerization
  • Euchromatin
  • Fluorescence Recovery After Photobleaching
  • HeLa Cells
  • Heterochromatin
  • Histones
  • Humans
  • Hydroxamic Acids
  • Kinetics
  • Methyltransferases
  • Mice
  • Mice, Knockout
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins
  • Transfection
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Cheutin, T., McNairn, A. J., Jenuwein, T., Gilbert, D. M., Singh, P. B., & Misteli, T. (2003). Maintenance of stable heterochromatin domains by dynamic HP1 binding. Science, 299(5607), 721-5. https://doi.org/10.1126/science.1078572

Maintenance of stable heterochromatin domains by dynamic HP1 binding. / Cheutin, Thierry; McNairn, Adrian J; Jenuwein, Thomas; Gilbert, David M; Singh, Prim B; Misteli, Tom.

In: Science, Vol. 299, No. 5607, 31.01.2003, p. 721-5.

Research output: Contribution to journalArticle

Cheutin, T, McNairn, AJ, Jenuwein, T, Gilbert, DM, Singh, PB & Misteli, T 2003, 'Maintenance of stable heterochromatin domains by dynamic HP1 binding', Science, vol. 299, no. 5607, pp. 721-5. https://doi.org/10.1126/science.1078572
Cheutin T, McNairn AJ, Jenuwein T, Gilbert DM, Singh PB, Misteli T. Maintenance of stable heterochromatin domains by dynamic HP1 binding. Science. 2003 Jan 31;299(5607):721-5. https://doi.org/10.1126/science.1078572
Cheutin, Thierry ; McNairn, Adrian J ; Jenuwein, Thomas ; Gilbert, David M ; Singh, Prim B ; Misteli, Tom. / Maintenance of stable heterochromatin domains by dynamic HP1 binding. In: Science. 2003 ; Vol. 299, No. 5607. pp. 721-5.
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