Mammalian Polycomb Scmh1 mediates exclusion of Polycomb complexes from the XY body in the pachytene spermatocytes

Yuki Takada, Kyo-ichi Isono, Jun Shinga, James M A Turner, Hiroshi Kitamura, Osamu Ohara, Gen Watanabe, Prim B Singh, Takehiko Kamijo, Thomas Jenuwein, Paul S Burgoyne, Haruhiko Koseki

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The product of the Scmh1 gene, a mammalian homolog of Drosophila Sex comb on midleg, is a constituent of the mammalian Polycomb repressive complexes 1 (Prc1). We have identified Scmh1 as an indispensable component of the Prc1. During progression through pachytene, Scmh1 was shown to be excluded from the XY body at late pachytene, together with other Prc1 components such as Phc1, Phc2, Rnf110 (Pcgf2), Bmi1 and Cbx2. We have identified the role of Scmh1 in mediating the survival of late pachytene spermatocytes. Apoptotic elimination of Scmh1(-/-) spermatocytes is accompanied by the preceding failure of several specific chromatin modifications at the XY body, whereas synapsis of homologous autosomes is not affected. It is therefore suggested that Scmh1 is involved in regulating the sequential changes in chromatin modifications at the XY chromatin domain of the pachytene spermatocytes. Restoration of defects in Scmh1(-/-) spermatocytes by Phc2 mutation indicates that Scmh1 exerts its molecular functions via its interaction with Prc1. Therefore, for the first time, we are able to indicate a functional involvement of Prc1 during the meiotic prophase of male germ cells and a regulatory role of Scmh1 for Prc1, which involves sex chromosomes.

Original languageEnglish
Pages (from-to)579-90
Number of pages12
JournalDevelopment
Volume134
Issue number3
DOIs
Publication statusPublished - Feb 2007

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Polycomb Repressive Complex 1
Spermatocytes
Chromatin
Chromosome Pairing
Prophase
Comb and Wattles
Sex Chromosomes
Germ Cells
Drosophila
Mutation

Keywords

  • Animals
  • Apoptosis
  • Base Sequence
  • DNA Primers
  • Histones
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mutation
  • Polycomb Repressive Complex 1
  • Polycomb-Group Proteins
  • Repressor Proteins
  • Spermatocytes
  • Spermatogenesis
  • Subcellular Fractions
  • X Chromosome
  • Y Chromosome
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Takada, Y., Isono, K., Shinga, J., Turner, J. M. A., Kitamura, H., Ohara, O., ... Koseki, H. (2007). Mammalian Polycomb Scmh1 mediates exclusion of Polycomb complexes from the XY body in the pachytene spermatocytes. Development, 134(3), 579-90. https://doi.org/10.1242/dev.02747

Mammalian Polycomb Scmh1 mediates exclusion of Polycomb complexes from the XY body in the pachytene spermatocytes. / Takada, Yuki; Isono, Kyo-ichi; Shinga, Jun; Turner, James M A; Kitamura, Hiroshi; Ohara, Osamu; Watanabe, Gen; Singh, Prim B; Kamijo, Takehiko; Jenuwein, Thomas; Burgoyne, Paul S; Koseki, Haruhiko.

In: Development, Vol. 134, No. 3, 02.2007, p. 579-90.

Research output: Contribution to journalArticle

Takada, Y, Isono, K, Shinga, J, Turner, JMA, Kitamura, H, Ohara, O, Watanabe, G, Singh, PB, Kamijo, T, Jenuwein, T, Burgoyne, PS & Koseki, H 2007, 'Mammalian Polycomb Scmh1 mediates exclusion of Polycomb complexes from the XY body in the pachytene spermatocytes', Development, vol. 134, no. 3, pp. 579-90. https://doi.org/10.1242/dev.02747
Takada, Yuki ; Isono, Kyo-ichi ; Shinga, Jun ; Turner, James M A ; Kitamura, Hiroshi ; Ohara, Osamu ; Watanabe, Gen ; Singh, Prim B ; Kamijo, Takehiko ; Jenuwein, Thomas ; Burgoyne, Paul S ; Koseki, Haruhiko. / Mammalian Polycomb Scmh1 mediates exclusion of Polycomb complexes from the XY body in the pachytene spermatocytes. In: Development. 2007 ; Vol. 134, No. 3. pp. 579-90.
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abstract = "The product of the Scmh1 gene, a mammalian homolog of Drosophila Sex comb on midleg, is a constituent of the mammalian Polycomb repressive complexes 1 (Prc1). We have identified Scmh1 as an indispensable component of the Prc1. During progression through pachytene, Scmh1 was shown to be excluded from the XY body at late pachytene, together with other Prc1 components such as Phc1, Phc2, Rnf110 (Pcgf2), Bmi1 and Cbx2. We have identified the role of Scmh1 in mediating the survival of late pachytene spermatocytes. Apoptotic elimination of Scmh1(-/-) spermatocytes is accompanied by the preceding failure of several specific chromatin modifications at the XY body, whereas synapsis of homologous autosomes is not affected. It is therefore suggested that Scmh1 is involved in regulating the sequential changes in chromatin modifications at the XY chromatin domain of the pachytene spermatocytes. Restoration of defects in Scmh1(-/-) spermatocytes by Phc2 mutation indicates that Scmh1 exerts its molecular functions via its interaction with Prc1. Therefore, for the first time, we are able to indicate a functional involvement of Prc1 during the meiotic prophase of male germ cells and a regulatory role of Scmh1 for Prc1, which involves sex chromosomes.",
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AU - Turner, James M A

AU - Kitamura, Hiroshi

AU - Ohara, Osamu

AU - Watanabe, Gen

AU - Singh, Prim B

AU - Kamijo, Takehiko

AU - Jenuwein, Thomas

AU - Burgoyne, Paul S

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AB - The product of the Scmh1 gene, a mammalian homolog of Drosophila Sex comb on midleg, is a constituent of the mammalian Polycomb repressive complexes 1 (Prc1). We have identified Scmh1 as an indispensable component of the Prc1. During progression through pachytene, Scmh1 was shown to be excluded from the XY body at late pachytene, together with other Prc1 components such as Phc1, Phc2, Rnf110 (Pcgf2), Bmi1 and Cbx2. We have identified the role of Scmh1 in mediating the survival of late pachytene spermatocytes. Apoptotic elimination of Scmh1(-/-) spermatocytes is accompanied by the preceding failure of several specific chromatin modifications at the XY body, whereas synapsis of homologous autosomes is not affected. It is therefore suggested that Scmh1 is involved in regulating the sequential changes in chromatin modifications at the XY chromatin domain of the pachytene spermatocytes. Restoration of defects in Scmh1(-/-) spermatocytes by Phc2 mutation indicates that Scmh1 exerts its molecular functions via its interaction with Prc1. Therefore, for the first time, we are able to indicate a functional involvement of Prc1 during the meiotic prophase of male germ cells and a regulatory role of Scmh1 for Prc1, which involves sex chromosomes.

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KW - Apoptosis

KW - Base Sequence

KW - DNA Primers

KW - Histones

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KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Mutation

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KW - Repressor Proteins

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KW - Spermatogenesis

KW - Subcellular Fractions

KW - X Chromosome

KW - Y Chromosome

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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JO - Development

JF - Development

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ER -