Measurement of pore size and porosity of tissue scaffolds

Paul Tomlins, Paul Grant, Sergey Mikhalovsky, Stuart James, Lyuba Mikhalovska

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

Tissue engineering involves seeding the patient's cells on to a three-dimensional temporary scaffold. It is becoming increasingly obvious that cells used to seed the scaffold have very specific requirements concerning the morphology and chemistry of the surface of the scaffold and its interconnectivity. A range of techniques has been examined in relation to key measurements such as pore size and porosity. Since capillary flow porometry measures a pore solely at its most constricted point, the method is unable to provide characterisation of other aspects of the pore. Scanning Electron Microscopy is limited to examining surface pores in 'stiff' scaffolds. Although cryo-SEM reduces the amount of ice-induced damage generated in 'soft' scaffolds upon freezing, the technique is limited to the same constraints. Images produced via scanning confocal microscopy are probably more representative of the true structure of the scaffold than that implied by cryo-SEM, although due to the diffuse nature of the image it is difficult to measure pore sizes.

Original languageEnglish
Title of host publicationASTM Special Technical Publication
PublisherAmerican Society for Testing and Materials
Pages3-11
Number of pages9
Edition1452
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

Scaffolds
Pore size
Porosity
Scanning electron microscopy
Capillary flow
Confocal microscopy
Tissue engineering
Freezing
Ice
Seed
Tissue Scaffolds
Scanning

Keywords

  • Characterisation
  • Pore size
  • Porosity
  • Tissue engineering
  • Tissue scaffolds

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Tomlins, P., Grant, P., Mikhalovsky, S., James, S., & Mikhalovska, L. (2004). Measurement of pore size and porosity of tissue scaffolds. In ASTM Special Technical Publication (1452 ed., pp. 3-11). American Society for Testing and Materials.

Measurement of pore size and porosity of tissue scaffolds. / Tomlins, Paul; Grant, Paul; Mikhalovsky, Sergey; James, Stuart; Mikhalovska, Lyuba.

ASTM Special Technical Publication. 1452. ed. American Society for Testing and Materials, 2004. p. 3-11.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tomlins, P, Grant, P, Mikhalovsky, S, James, S & Mikhalovska, L 2004, Measurement of pore size and porosity of tissue scaffolds. in ASTM Special Technical Publication. 1452 edn, American Society for Testing and Materials, pp. 3-11.
Tomlins P, Grant P, Mikhalovsky S, James S, Mikhalovska L. Measurement of pore size and porosity of tissue scaffolds. In ASTM Special Technical Publication. 1452 ed. American Society for Testing and Materials. 2004. p. 3-11
Tomlins, Paul ; Grant, Paul ; Mikhalovsky, Sergey ; James, Stuart ; Mikhalovska, Lyuba. / Measurement of pore size and porosity of tissue scaffolds. ASTM Special Technical Publication. 1452. ed. American Society for Testing and Materials, 2004. pp. 3-11
@inproceedings{e0ebd2d63f504f5c9ea0f6a948c410d8,
title = "Measurement of pore size and porosity of tissue scaffolds",
abstract = "Tissue engineering involves seeding the patient's cells on to a three-dimensional temporary scaffold. It is becoming increasingly obvious that cells used to seed the scaffold have very specific requirements concerning the morphology and chemistry of the surface of the scaffold and its interconnectivity. A range of techniques has been examined in relation to key measurements such as pore size and porosity. Since capillary flow porometry measures a pore solely at its most constricted point, the method is unable to provide characterisation of other aspects of the pore. Scanning Electron Microscopy is limited to examining surface pores in 'stiff' scaffolds. Although cryo-SEM reduces the amount of ice-induced damage generated in 'soft' scaffolds upon freezing, the technique is limited to the same constraints. Images produced via scanning confocal microscopy are probably more representative of the true structure of the scaffold than that implied by cryo-SEM, although due to the diffuse nature of the image it is difficult to measure pore sizes.",
keywords = "Characterisation, Pore size, Porosity, Tissue engineering, Tissue scaffolds",
author = "Paul Tomlins and Paul Grant and Sergey Mikhalovsky and Stuart James and Lyuba Mikhalovska",
year = "2004",
language = "English",
pages = "3--11",
booktitle = "ASTM Special Technical Publication",
publisher = "American Society for Testing and Materials",
address = "United States",
edition = "1452",

}

TY - GEN

T1 - Measurement of pore size and porosity of tissue scaffolds

AU - Tomlins, Paul

AU - Grant, Paul

AU - Mikhalovsky, Sergey

AU - James, Stuart

AU - Mikhalovska, Lyuba

PY - 2004

Y1 - 2004

N2 - Tissue engineering involves seeding the patient's cells on to a three-dimensional temporary scaffold. It is becoming increasingly obvious that cells used to seed the scaffold have very specific requirements concerning the morphology and chemistry of the surface of the scaffold and its interconnectivity. A range of techniques has been examined in relation to key measurements such as pore size and porosity. Since capillary flow porometry measures a pore solely at its most constricted point, the method is unable to provide characterisation of other aspects of the pore. Scanning Electron Microscopy is limited to examining surface pores in 'stiff' scaffolds. Although cryo-SEM reduces the amount of ice-induced damage generated in 'soft' scaffolds upon freezing, the technique is limited to the same constraints. Images produced via scanning confocal microscopy are probably more representative of the true structure of the scaffold than that implied by cryo-SEM, although due to the diffuse nature of the image it is difficult to measure pore sizes.

AB - Tissue engineering involves seeding the patient's cells on to a three-dimensional temporary scaffold. It is becoming increasingly obvious that cells used to seed the scaffold have very specific requirements concerning the morphology and chemistry of the surface of the scaffold and its interconnectivity. A range of techniques has been examined in relation to key measurements such as pore size and porosity. Since capillary flow porometry measures a pore solely at its most constricted point, the method is unable to provide characterisation of other aspects of the pore. Scanning Electron Microscopy is limited to examining surface pores in 'stiff' scaffolds. Although cryo-SEM reduces the amount of ice-induced damage generated in 'soft' scaffolds upon freezing, the technique is limited to the same constraints. Images produced via scanning confocal microscopy are probably more representative of the true structure of the scaffold than that implied by cryo-SEM, although due to the diffuse nature of the image it is difficult to measure pore sizes.

KW - Characterisation

KW - Pore size

KW - Porosity

KW - Tissue engineering

KW - Tissue scaffolds

UR - http://www.scopus.com/inward/record.url?scp=3242705966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3242705966&partnerID=8YFLogxK

M3 - Conference contribution

SP - 3

EP - 11

BT - ASTM Special Technical Publication

PB - American Society for Testing and Materials

ER -