Membrane IgE binds and activates FcεRI in an antigen-independent manner

Luca Vangelista, Elisa Soprana, Michela Cesco-Gaspere, Paola Mandiola, Giulia Di Lullo, Rita N. Fucci, Franca Codazzi, Alessio Palini, Giovanni Paganelli, Oscar R. Burrone, Antonio G. Siccardi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Interaction of secretory IgE with FcεRI Is the prerequisite for allergen-driven cellular responses, fundamental events in immediate and chronic allergic manifestations. Previous studies reported the binding of soluble FcεRIα to membrane IgE exposed on B cells. In this study, the functional Interaction between human membrane IgE and human FcεRI is presented. Four different IgE versions were expressed in mouse B cell lines, namely: a truncation at the Cε2-Cε3 junction of membrane IgE isoform long, membrane IgE isoform long (without Igα/Igβ BCR accessory proteins), and both εBCRs (containing membrane IgE isoforms short and long). All membrane IgE versions activated a rat basophilic leukemia cell line transfected with human FcεRI, as detected by measuring the release of both preformed and newly synthesized mediators. The interaction led also to Ca 2+ responses in the basophil cell line, while membrane IgE-FcεRI complexes were detected by immunoprecipitation. FcεRI activation by membrane IgE occurs in an Ag-independent manner. Noteworthily, human peripheral blood basophils and monocytes also were activated upon contact with cells bearing membrane IgE. In humans, the presence of FcεRI in several cellular entities suggests a possible membrane IgE-FcεRI-driven cell-cell dialogue, with likely implications for IgE homeostasis in physiology and pathology.

Original languageEnglish
Pages (from-to)5602-5611
Number of pages10
JournalJournal of Immunology
Volume174
Issue number9
Publication statusPublished - May 1 2005
Externally publishedYes

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Immunoglobulin E
Antigens
Membranes
Protein Isoforms
Basophils
Cell Line
B-Lymphocytes
Immunoprecipitation
Allergens
Monocytes
Leukemia
Homeostasis
Cell Membrane
Pathology

ASJC Scopus subject areas

  • Immunology

Cite this

Vangelista, L., Soprana, E., Cesco-Gaspere, M., Mandiola, P., Lullo, G. D., Fucci, R. N., ... Siccardi, A. G. (2005). Membrane IgE binds and activates FcεRI in an antigen-independent manner. Journal of Immunology, 174(9), 5602-5611.

Membrane IgE binds and activates FcεRI in an antigen-independent manner. / Vangelista, Luca; Soprana, Elisa; Cesco-Gaspere, Michela; Mandiola, Paola; Lullo, Giulia Di; Fucci, Rita N.; Codazzi, Franca; Palini, Alessio; Paganelli, Giovanni; Burrone, Oscar R.; Siccardi, Antonio G.

In: Journal of Immunology, Vol. 174, No. 9, 01.05.2005, p. 5602-5611.

Research output: Contribution to journalArticle

Vangelista, L, Soprana, E, Cesco-Gaspere, M, Mandiola, P, Lullo, GD, Fucci, RN, Codazzi, F, Palini, A, Paganelli, G, Burrone, OR & Siccardi, AG 2005, 'Membrane IgE binds and activates FcεRI in an antigen-independent manner', Journal of Immunology, vol. 174, no. 9, pp. 5602-5611.
Vangelista L, Soprana E, Cesco-Gaspere M, Mandiola P, Lullo GD, Fucci RN et al. Membrane IgE binds and activates FcεRI in an antigen-independent manner. Journal of Immunology. 2005 May 1;174(9):5602-5611.
Vangelista, Luca ; Soprana, Elisa ; Cesco-Gaspere, Michela ; Mandiola, Paola ; Lullo, Giulia Di ; Fucci, Rita N. ; Codazzi, Franca ; Palini, Alessio ; Paganelli, Giovanni ; Burrone, Oscar R. ; Siccardi, Antonio G. / Membrane IgE binds and activates FcεRI in an antigen-independent manner. In: Journal of Immunology. 2005 ; Vol. 174, No. 9. pp. 5602-5611.
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