Abstract
Objective Changes in the gut microbiota are increasingly recognised to be involved in many diseases. This ecosystem is known to be shaped by many factors, including climate, geography, host nutrition, lifestyle and medication. Thus, knowledge of varying populations with different habits is important for a better understanding of the microbiome. Design We therefore conducted a metagenomic analysis of intestinal microbiota from Kazakh donors, recruiting 84 subjects, including male and female healthy subjects and metabolic syndrome (MetS) patients aged 25-75 years, from the Kazakh administrative centre, Astana. We characterise and describe these microbiomes, the first deep-sequencing cohort from Central Asia, in comparison with a global dataset (832 individuals from five countries on three continents), and explore correlations between microbiota, clinical and laboratory parameters as well as with nutritional data from Food Frequency Questionnaires. Results We observe that Kazakh microbiomes are relatively different from both European and East Asian counterparts, though similar to other Central Asian microbiomes, with the most striking difference being significantly more samples falling within the Prevotella-rich enterotype, potentially reflecting regional diet and lifestyle. We show that this enterotype designation remains stable within an individual over time in 82% of cases. We further observe gut microbiome features that distinguish MetS patients from controls (eg, significantly reduced Firmicutes to Bacteroidetes ratio, Bifidobacteria and Subdoligranulum, alongside increased Prevotella), though these overlap little with previously published reports and thus may reflect idiosyncrasies of the present cohort. Conclusion Taken together, this exploratory study describes gut microbiome data from an understudied population, providing a starting point for further comparative work on biogeography and research on widespread diseases. Trial registration number ISRCTN37346212; Post-results.
| Original language | English |
|---|---|
| Article number | e021682 |
| Journal | BMJ Open |
| Volume | 8 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 1 2018 |
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Keywords
- Gut Microbiome
- Metagenomics
- Probiotic
ASJC Scopus subject areas
- Medicine(all)
Cite this
Metagenomic analysis of gut microbial communities from a Central Asian population. / Kushugulova, Almagul; Forslund, Sofia K.; Costea, Paul Igor; Kozhakhmetov, Samat; Khassenbekova, Zhanagul; Urazova, Maira; Nurgozhin, Talgat; Zhumadilov, Zhaxybay; Benberin, Valery; Driessen, Marja; Hercog, Rajna; Voigt, Anita Yvonne; Benes, Vladimir; Kandels-Lewis, Stefanie; Sunagawa, Shinichi; Letunic, Ivica; Bork, Peer.
In: BMJ Open, Vol. 8, No. 7, e021682, 01.07.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Metagenomic analysis of gut microbial communities from a Central Asian population
AU - Kushugulova, Almagul
AU - Forslund, Sofia K.
AU - Costea, Paul Igor
AU - Kozhakhmetov, Samat
AU - Khassenbekova, Zhanagul
AU - Urazova, Maira
AU - Nurgozhin, Talgat
AU - Zhumadilov, Zhaxybay
AU - Benberin, Valery
AU - Driessen, Marja
AU - Hercog, Rajna
AU - Voigt, Anita Yvonne
AU - Benes, Vladimir
AU - Kandels-Lewis, Stefanie
AU - Sunagawa, Shinichi
AU - Letunic, Ivica
AU - Bork, Peer
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Objective Changes in the gut microbiota are increasingly recognised to be involved in many diseases. This ecosystem is known to be shaped by many factors, including climate, geography, host nutrition, lifestyle and medication. Thus, knowledge of varying populations with different habits is important for a better understanding of the microbiome. Design We therefore conducted a metagenomic analysis of intestinal microbiota from Kazakh donors, recruiting 84 subjects, including male and female healthy subjects and metabolic syndrome (MetS) patients aged 25-75 years, from the Kazakh administrative centre, Astana. We characterise and describe these microbiomes, the first deep-sequencing cohort from Central Asia, in comparison with a global dataset (832 individuals from five countries on three continents), and explore correlations between microbiota, clinical and laboratory parameters as well as with nutritional data from Food Frequency Questionnaires. Results We observe that Kazakh microbiomes are relatively different from both European and East Asian counterparts, though similar to other Central Asian microbiomes, with the most striking difference being significantly more samples falling within the Prevotella-rich enterotype, potentially reflecting regional diet and lifestyle. We show that this enterotype designation remains stable within an individual over time in 82% of cases. We further observe gut microbiome features that distinguish MetS patients from controls (eg, significantly reduced Firmicutes to Bacteroidetes ratio, Bifidobacteria and Subdoligranulum, alongside increased Prevotella), though these overlap little with previously published reports and thus may reflect idiosyncrasies of the present cohort. Conclusion Taken together, this exploratory study describes gut microbiome data from an understudied population, providing a starting point for further comparative work on biogeography and research on widespread diseases. Trial registration number ISRCTN37346212; Post-results.
AB - Objective Changes in the gut microbiota are increasingly recognised to be involved in many diseases. This ecosystem is known to be shaped by many factors, including climate, geography, host nutrition, lifestyle and medication. Thus, knowledge of varying populations with different habits is important for a better understanding of the microbiome. Design We therefore conducted a metagenomic analysis of intestinal microbiota from Kazakh donors, recruiting 84 subjects, including male and female healthy subjects and metabolic syndrome (MetS) patients aged 25-75 years, from the Kazakh administrative centre, Astana. We characterise and describe these microbiomes, the first deep-sequencing cohort from Central Asia, in comparison with a global dataset (832 individuals from five countries on three continents), and explore correlations between microbiota, clinical and laboratory parameters as well as with nutritional data from Food Frequency Questionnaires. Results We observe that Kazakh microbiomes are relatively different from both European and East Asian counterparts, though similar to other Central Asian microbiomes, with the most striking difference being significantly more samples falling within the Prevotella-rich enterotype, potentially reflecting regional diet and lifestyle. We show that this enterotype designation remains stable within an individual over time in 82% of cases. We further observe gut microbiome features that distinguish MetS patients from controls (eg, significantly reduced Firmicutes to Bacteroidetes ratio, Bifidobacteria and Subdoligranulum, alongside increased Prevotella), though these overlap little with previously published reports and thus may reflect idiosyncrasies of the present cohort. Conclusion Taken together, this exploratory study describes gut microbiome data from an understudied population, providing a starting point for further comparative work on biogeography and research on widespread diseases. Trial registration number ISRCTN37346212; Post-results.
KW - Gut Microbiome
KW - Metagenomics
KW - Probiotic
UR - http://www.scopus.com/inward/record.url?scp=85053051283&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85053051283&partnerID=8YFLogxK
U2 - 10.1136/bmjopen-2018-021682
DO - 10.1136/bmjopen-2018-021682
M3 - Article
VL - 8
JO - BMJ Open
JF - BMJ Open
SN - 2044-6055
IS - 7
M1 - e021682
ER -