Microcontact-BSA imprinted capacitive biosensor for real-time, sensitive and selective detection of BSA

Gizem Ertürk, Dmitriy Berillo, Martin Hedström, Bo Mattiasson

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the protein stamp and the gold electrode into contact under UV-polymerization. Real-time BSA detection studies were performed in the concentration range of 1.0 × 10-20-1.0 × 10-8 M with a limit of detection (LOD) of 1.0 × 10-19 M. Cross-reactivity towards HSA and IgG were 5 and 3%, respectively. The electrodes were used for >70 assays during 2 months and retained their binding properties during all that time. The NIP (non-imprinted) electrode was used as a reference. The microcontact imprinting technology combined with the biosensor applications is a promising technology for future applications.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalBiotechnology Reports
Volume3
DOIs
Publication statusPublished - Sep 2014

Keywords

  • BSA detection
  • Capacitive biosensor
  • Microcontact imprinting

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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