Abstract
An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the protein stamp and the gold electrode into contact under UV-polymerization. Real-time BSA detection studies were performed in the concentration range of 1.0 × 10-20-1.0 × 10-8 M with a limit of detection (LOD) of 1.0 × 10-19 M. Cross-reactivity towards HSA and IgG were 5 and 3%, respectively. The electrodes were used for >70 assays during 2 months and retained their binding properties during all that time. The NIP (non-imprinted) electrode was used as a reference. The microcontact imprinting technology combined with the biosensor applications is a promising technology for future applications.
| Original language | English |
|---|---|
| Pages (from-to) | 65-72 |
| Number of pages | 8 |
| Journal | Biotechnology Reports |
| Volume | 3 |
| DOIs | |
| Publication status | Published - 2014 |
| Externally published | Yes |
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Keywords
- BSA detection
- Capacitive biosensor
- Microcontact imprinting
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Microcontact-BSA imprinted capacitive biosensor for real-time, sensitive and selective detection of BSA. / Ertürk, Gizem; Berillo, Dmitriy; Hedström, Martin; Mattiasson, Bo.
In: Biotechnology Reports, Vol. 3, 2014, p. 65-72.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microcontact-BSA imprinted capacitive biosensor for real-time, sensitive and selective detection of BSA
AU - Ertürk, Gizem
AU - Berillo, Dmitriy
AU - Hedström, Martin
AU - Mattiasson, Bo
PY - 2014
Y1 - 2014
N2 - An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the protein stamp and the gold electrode into contact under UV-polymerization. Real-time BSA detection studies were performed in the concentration range of 1.0 × 10-20-1.0 × 10-8 M with a limit of detection (LOD) of 1.0 × 10-19 M. Cross-reactivity towards HSA and IgG were 5 and 3%, respectively. The electrodes were used for >70 assays during 2 months and retained their binding properties during all that time. The NIP (non-imprinted) electrode was used as a reference. The microcontact imprinting technology combined with the biosensor applications is a promising technology for future applications.
AB - An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the protein stamp and the gold electrode into contact under UV-polymerization. Real-time BSA detection studies were performed in the concentration range of 1.0 × 10-20-1.0 × 10-8 M with a limit of detection (LOD) of 1.0 × 10-19 M. Cross-reactivity towards HSA and IgG were 5 and 3%, respectively. The electrodes were used for >70 assays during 2 months and retained their binding properties during all that time. The NIP (non-imprinted) electrode was used as a reference. The microcontact imprinting technology combined with the biosensor applications is a promising technology for future applications.
KW - BSA detection
KW - Capacitive biosensor
KW - Microcontact imprinting
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UR - http://www.scopus.com/inward/citedby.url?scp=84904895683&partnerID=8YFLogxK
U2 - 10.1016/j.btre.2014.06.006
DO - 10.1016/j.btre.2014.06.006
M3 - Article
VL - 3
SP - 65
EP - 72
JO - Biotechnology Reports
JF - Biotechnology Reports
SN - 2215-017X
ER -