TY - JOUR
T1 - Microcontact-BSA imprinted capacitive biosensor for real-time, sensitive and selective detection of BSA
AU - Ertürk, Gizem
AU - Berillo, Dmitriy
AU - Hedström, Martin
AU - Mattiasson, Bo
N1 - Funding Information:
The project was also supported by the Swedish Research Council and the instrument used for analysis was a loan from CapSenze HB, Lund, Sweden.
Funding Information:
GE was supported by a fellowship from Hacettepe University , Turkey. The support from Prof. Adil Denizli and Prof. M. Aşkın Tümer, both at Hacettepe University, is gratefully acknowledged.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/9
Y1 - 2014/9
N2 - An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the protein stamp and the gold electrode into contact under UV-polymerization. Real-time BSA detection studies were performed in the concentration range of 1.0 × 10-20-1.0 × 10-8 M with a limit of detection (LOD) of 1.0 × 10-19 M. Cross-reactivity towards HSA and IgG were 5 and 3%, respectively. The electrodes were used for >70 assays during 2 months and retained their binding properties during all that time. The NIP (non-imprinted) electrode was used as a reference. The microcontact imprinting technology combined with the biosensor applications is a promising technology for future applications.
AB - An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the protein stamp and the gold electrode into contact under UV-polymerization. Real-time BSA detection studies were performed in the concentration range of 1.0 × 10-20-1.0 × 10-8 M with a limit of detection (LOD) of 1.0 × 10-19 M. Cross-reactivity towards HSA and IgG were 5 and 3%, respectively. The electrodes were used for >70 assays during 2 months and retained their binding properties during all that time. The NIP (non-imprinted) electrode was used as a reference. The microcontact imprinting technology combined with the biosensor applications is a promising technology for future applications.
KW - BSA detection
KW - Capacitive biosensor
KW - Microcontact imprinting
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U2 - 10.1016/j.btre.2014.06.006
DO - 10.1016/j.btre.2014.06.006
M3 - Article
AN - SCOPUS:84904895683
VL - 3
SP - 65
EP - 72
JO - Biotechnology Reports
JF - Biotechnology Reports
SN - 2215-017X
ER -