Micropatterned co-cultures of T-lymphocytes and epithelial cells as a model of mucosal immune system

Gulnaz Stybayeva, He Zhu, Erlan Ramanculov, Satya Dandekar, Michael George, Alexander Revzin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Gut-associated lymphoid tissue is a major target and reservoir of human immunodeficiency virus (HIV)-infected T-cells. Our studies seek to recapitulate, in vitro, interactions between HIV-infected T-lymphocytes and intestinal epithelial cells in order to investigate the mechanisms underlying the disruption of normal epithelial cell and barrier function. Here, we describe a novel approach for creating co-cultures of healthy or HIV-infected T-lymphocytes (Jurkat) and human intestinal epithelial (HT-29) cells where both cell types are positioned on the same surface in a price spatial configuration (micropattern). This co-culture method simplified observation/monitoring of the two cell types and was particularly suited for laser microdissection-based retrieval of the desired cells for downstream gene expressions studies. DNA microarray analysis of epithelial cells retrieved from co-cultures with HIV-1-infected vs. uninfected Jurkat cells revealed that epithelial cells from HIV-infected co-cultures exhibited gene expression patterns consistent with disruption of epithelial barrier formation. Overall, the micropatterned co-culture system described here is envisioned as a valuable new tool for delineating how HIV and other infections contribute to dysfunction of mucosal epithelium.

Original languageEnglish
Pages (from-to)575-580
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume380
Issue number3
DOIs
Publication statusPublished - Mar 13 2009
Externally publishedYes

Fingerprint

T-cells
Immune system
Coculture Techniques
Viruses
Cell culture
Immune System
Epithelial Cells
HIV
T-Lymphocytes
Gene expression
Microdissection
Gene Expression
HT29 Cells
Jurkat Cells
Lymphoid Tissue
Virus Diseases
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Microarrays
HIV-1

Keywords

  • Antibody microarrays
  • Epithelial cells
  • HIV enteropathy
  • Micropatterned co-cultures
  • Mucosal immune system
  • T-lymphocytes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Micropatterned co-cultures of T-lymphocytes and epithelial cells as a model of mucosal immune system. / Stybayeva, Gulnaz; Zhu, He; Ramanculov, Erlan; Dandekar, Satya; George, Michael; Revzin, Alexander.

In: Biochemical and Biophysical Research Communications, Vol. 380, No. 3, 13.03.2009, p. 575-580.

Research output: Contribution to journalArticle

Stybayeva, Gulnaz ; Zhu, He ; Ramanculov, Erlan ; Dandekar, Satya ; George, Michael ; Revzin, Alexander. / Micropatterned co-cultures of T-lymphocytes and epithelial cells as a model of mucosal immune system. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 380, No. 3. pp. 575-580.
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