Microtubules suppress blebbing and stimulate lamellae extension in spreading fibroblasts

A. V. Tvorogova, I. A. Vorobjev

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We compared spreading of Vero fibroblasts when microtubules were depolymerized or stabilized. After initial attachment cells start blebbing that continues for different time and abruptly transfers into spreading. After spreading initiation, most cells spread in an anisotropic manner through stochastic formation of lamellipo-dia. A second mode was rapid, isotropic spreading via formation of circular lamellum that occurs in 15 % of cells. The rate of spreading was maximal at the beginning and decreased during the first hour according to logarithmic law. After 60 min many cells formed stable efges and started migrating on the substrate. However, cell area slowly continued to increase. Actin bundles are formed 20 min after cell attachment and they first run along cell boundary. This system disassembles within 20-40 min and is substituted with stress fibers crossing the cell. In the isotropically spread cells no actin bunbles are seen. Microtubules in the spreading cells enter into large blebs and all nascent lamella and later form radial array. When MTs has been depolymerized or stabilized blebbing started before cells attached to the substrate and continue much longer than in control cells. In both cases the initial rate of spreading decrease several fold, and remains constant for many hours. After 24 h the mean area occupied by cells with altered MT system was the same as in control. Alteration of MT system had moderate effect on actin system - formation of actin cables started at the same time as in control (within 20 min upon cell attachment), however, they grew even in cells undergoing prolonged blebbing. Actin cables running along cell margin were similar to tat in control cells, but they did not disappear up to 1 h. When stabilized, microtubules form chaotic array: they do not enter blebs and in spread cells run parallel to the cell margin at a distance of 3-5 μm. We conclude that dynamic microtubules speed up completion of blebbing and promote early stages of fibroblasts spreading.

Original languageEnglish
Pages (from-to)742-753
Number of pages12
JournalTsitologiya
Volume54
Issue number10
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Blister
Microtubules
Fibroblasts
Actins
Stress Fibers

Keywords

  • Actin
  • Fibroblasts
  • Microscopy
  • Microtubules
  • Spreading

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

Microtubules suppress blebbing and stimulate lamellae extension in spreading fibroblasts. / Tvorogova, A. V.; Vorobjev, I. A.

In: Tsitologiya, Vol. 54, No. 10, 2012, p. 742-753.

Research output: Contribution to journalArticle

@article{a5fc0f4fd3204541960987f8bb4abd41,
title = "Microtubules suppress blebbing and stimulate lamellae extension in spreading fibroblasts",
abstract = "We compared spreading of Vero fibroblasts when microtubules were depolymerized or stabilized. After initial attachment cells start blebbing that continues for different time and abruptly transfers into spreading. After spreading initiation, most cells spread in an anisotropic manner through stochastic formation of lamellipo-dia. A second mode was rapid, isotropic spreading via formation of circular lamellum that occurs in 15 {\%} of cells. The rate of spreading was maximal at the beginning and decreased during the first hour according to logarithmic law. After 60 min many cells formed stable efges and started migrating on the substrate. However, cell area slowly continued to increase. Actin bundles are formed 20 min after cell attachment and they first run along cell boundary. This system disassembles within 20-40 min and is substituted with stress fibers crossing the cell. In the isotropically spread cells no actin bunbles are seen. Microtubules in the spreading cells enter into large blebs and all nascent lamella and later form radial array. When MTs has been depolymerized or stabilized blebbing started before cells attached to the substrate and continue much longer than in control cells. In both cases the initial rate of spreading decrease several fold, and remains constant for many hours. After 24 h the mean area occupied by cells with altered MT system was the same as in control. Alteration of MT system had moderate effect on actin system - formation of actin cables started at the same time as in control (within 20 min upon cell attachment), however, they grew even in cells undergoing prolonged blebbing. Actin cables running along cell margin were similar to tat in control cells, but they did not disappear up to 1 h. When stabilized, microtubules form chaotic array: they do not enter blebs and in spread cells run parallel to the cell margin at a distance of 3-5 μm. We conclude that dynamic microtubules speed up completion of blebbing and promote early stages of fibroblasts spreading.",
keywords = "Actin, Fibroblasts, Microscopy, Microtubules, Spreading",
author = "Tvorogova, {A. V.} and Vorobjev, {I. A.}",
year = "2012",
language = "English",
volume = "54",
pages = "742--753",
journal = "Tsitologiya",
issn = "0041-3771",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "10",

}

TY - JOUR

T1 - Microtubules suppress blebbing and stimulate lamellae extension in spreading fibroblasts

AU - Tvorogova, A. V.

AU - Vorobjev, I. A.

PY - 2012

Y1 - 2012

N2 - We compared spreading of Vero fibroblasts when microtubules were depolymerized or stabilized. After initial attachment cells start blebbing that continues for different time and abruptly transfers into spreading. After spreading initiation, most cells spread in an anisotropic manner through stochastic formation of lamellipo-dia. A second mode was rapid, isotropic spreading via formation of circular lamellum that occurs in 15 % of cells. The rate of spreading was maximal at the beginning and decreased during the first hour according to logarithmic law. After 60 min many cells formed stable efges and started migrating on the substrate. However, cell area slowly continued to increase. Actin bundles are formed 20 min after cell attachment and they first run along cell boundary. This system disassembles within 20-40 min and is substituted with stress fibers crossing the cell. In the isotropically spread cells no actin bunbles are seen. Microtubules in the spreading cells enter into large blebs and all nascent lamella and later form radial array. When MTs has been depolymerized or stabilized blebbing started before cells attached to the substrate and continue much longer than in control cells. In both cases the initial rate of spreading decrease several fold, and remains constant for many hours. After 24 h the mean area occupied by cells with altered MT system was the same as in control. Alteration of MT system had moderate effect on actin system - formation of actin cables started at the same time as in control (within 20 min upon cell attachment), however, they grew even in cells undergoing prolonged blebbing. Actin cables running along cell margin were similar to tat in control cells, but they did not disappear up to 1 h. When stabilized, microtubules form chaotic array: they do not enter blebs and in spread cells run parallel to the cell margin at a distance of 3-5 μm. We conclude that dynamic microtubules speed up completion of blebbing and promote early stages of fibroblasts spreading.

AB - We compared spreading of Vero fibroblasts when microtubules were depolymerized or stabilized. After initial attachment cells start blebbing that continues for different time and abruptly transfers into spreading. After spreading initiation, most cells spread in an anisotropic manner through stochastic formation of lamellipo-dia. A second mode was rapid, isotropic spreading via formation of circular lamellum that occurs in 15 % of cells. The rate of spreading was maximal at the beginning and decreased during the first hour according to logarithmic law. After 60 min many cells formed stable efges and started migrating on the substrate. However, cell area slowly continued to increase. Actin bundles are formed 20 min after cell attachment and they first run along cell boundary. This system disassembles within 20-40 min and is substituted with stress fibers crossing the cell. In the isotropically spread cells no actin bunbles are seen. Microtubules in the spreading cells enter into large blebs and all nascent lamella and later form radial array. When MTs has been depolymerized or stabilized blebbing started before cells attached to the substrate and continue much longer than in control cells. In both cases the initial rate of spreading decrease several fold, and remains constant for many hours. After 24 h the mean area occupied by cells with altered MT system was the same as in control. Alteration of MT system had moderate effect on actin system - formation of actin cables started at the same time as in control (within 20 min upon cell attachment), however, they grew even in cells undergoing prolonged blebbing. Actin cables running along cell margin were similar to tat in control cells, but they did not disappear up to 1 h. When stabilized, microtubules form chaotic array: they do not enter blebs and in spread cells run parallel to the cell margin at a distance of 3-5 μm. We conclude that dynamic microtubules speed up completion of blebbing and promote early stages of fibroblasts spreading.

KW - Actin

KW - Fibroblasts

KW - Microscopy

KW - Microtubules

KW - Spreading

UR - http://www.scopus.com/inward/record.url?scp=84873732645&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873732645&partnerID=8YFLogxK

M3 - Article

C2 - 23285727

AN - SCOPUS:84873732645

VL - 54

SP - 742

EP - 753

JO - Tsitologiya

JF - Tsitologiya

SN - 0041-3771

IS - 10

ER -