Mimicking cascading liver metabolic reactions on a microfluidic platform

G. Kulsharova, P. Panjan, Y. Y. Chiang, N. Dimov, M. P.C. Marques, A. M. Sesay, F. Baganz, N. Szita

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We describe a novel microfluidic electrochemical biosensor chip as a first step towards the development of a microfluidic enzymatic system capable of rapidly analyzing cascading metabolic reactions in the liver. The model enzymatic cascade is based on the essential human metabolism of antimalarial drugs by the cytochrome P450 (P450) enzyme and glucosyltransferase (UGT) enzymes. Here we report the first part of the system: a P450 chip integrated with a novel redox biosensor for monitoring enzyme-substrate binding. This microfluidic platform allows successful enzyme-substrate detection and can predict enzymatic activity in continuous flow.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages539-540
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - Jan 1 2016
Externally publishedYes
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: Oct 9 2016Oct 13 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period10/9/1610/13/16

Keywords

  • Electrochemical microfluidic platform
  • Mimicking liver metabolism
  • P450 chip

ASJC Scopus subject areas

  • Control and Systems Engineering

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