Mitochondrial complex IV mutation increases reactive oxygen species production and reduces lifespan in aged mice

Gesine Reichart, Johannes Mayer, Cindy Zehm, Timo Kirschstein, Tursonjan Tokay, Falko Lange, Simone Baltrusch, Markus Tiedge, Georg Fuellen, Saleh Ibrahim, Rüdiger Köhling

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aim: Mitochondrial DNA (mtDNA) mutations can negatively influence lifespan and organ function. More than 250 pathogenic mtDNA mutations are known, often involving neurological symptoms. Major neurodegenerative diseases share key etiopathogenetic components ie mtDNA mutations, mitochondrial dysfunction and oxidative stress. Methods: Here, we characterized a conplastic mouse strain (C57BL/6 J-mtNOD) carrying an electron transport chain complex IV mutation that leads to an altered cytochrome c oxidase subunit III. Since this mouse also harbours adenine insertions in the mitochondrial tRNA for arginine, we chose the C57BL/6 J-mtMRL as control strain which also carries a heteroplasmic stretch of adenine repetitions in this tRNA isoform. Results: Using MitoSOX fluorescence, we observed an elevated mitochondrial superoxide production and a reduced gene expression of superoxide dismutase 2 in the 24-month-old mtNOD mouse as compared to control. Together with the decreased expression of the fission-relevant gene Fis1, these data confirmed that the ageing mtNOD mouse had a mitochondrial dysfunctional phenotype. On the functional level, we could not detect significant differences in synaptic long-term potentiation, but found a markedly poor physical constitution to perform the Morris water maze task at the age of 24 months. Moreover, the median lifespan of mtNOD mice was significantly shorter than of control animals. Conclusion: Our findings demonstrate that a complex IV mutation leads to mitochondrial dysfunction that translates into survival.

Original languageEnglish
Article numbere13214
JournalActa Physiologica
DOIs
Publication statusAccepted/In press - Jan 1 2018

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Reactive Oxygen Species
Mutation
Mitochondrial DNA
Electron Transport Complex IV
Adenine
Transfer RNA
Long-Term Potentiation
Constitution and Bylaws
Inbred C57BL Mouse
Superoxides
Neurodegenerative Diseases
Arginine
Protein Isoforms
Oxidative Stress
Fluorescence
Phenotype
Gene Expression
Water
Genes

Keywords

  • complex IV mutation
  • FIS1
  • lifespan
  • mitochondrial DNA
  • reactive oxygen species
  • SOD2

ASJC Scopus subject areas

  • Physiology

Cite this

Mitochondrial complex IV mutation increases reactive oxygen species production and reduces lifespan in aged mice. / Reichart, Gesine; Mayer, Johannes; Zehm, Cindy; Kirschstein, Timo; Tokay, Tursonjan; Lange, Falko; Baltrusch, Simone; Tiedge, Markus; Fuellen, Georg; Ibrahim, Saleh; Köhling, Rüdiger.

In: Acta Physiologica, 01.01.2018.

Research output: Contribution to journalArticle

Reichart, G, Mayer, J, Zehm, C, Kirschstein, T, Tokay, T, Lange, F, Baltrusch, S, Tiedge, M, Fuellen, G, Ibrahim, S & Köhling, R 2018, 'Mitochondrial complex IV mutation increases reactive oxygen species production and reduces lifespan in aged mice', Acta Physiologica. https://doi.org/10.1111/apha.13214
Reichart, Gesine ; Mayer, Johannes ; Zehm, Cindy ; Kirschstein, Timo ; Tokay, Tursonjan ; Lange, Falko ; Baltrusch, Simone ; Tiedge, Markus ; Fuellen, Georg ; Ibrahim, Saleh ; Köhling, Rüdiger. / Mitochondrial complex IV mutation increases reactive oxygen species production and reduces lifespan in aged mice. In: Acta Physiologica. 2018.
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