Modeling of the photosynthetic electron transport regulation in cyanobacteria

A. V. Vershubskii, V. I. Mishanin, A. N. Tikhonov

Research output: Contribution to journalArticle

Abstract

In this work we have performed a computer analysis of the electron and proton transport in cyanobacteria cells using a mathematical model of light-dependent stages of photosynthesis, taking into account key stages of pH-dependent regulation of electron transport on both acceptor and donor sites of photosystem 1 (PS1). The comparison of theoretical and experimental data proves that the model adequately describes the multiphase kinetics of photoinduced redox transformations of P700 (primary electron donor in PS 1). Our computer simulation describes an impact of variations of the atmosphere gases (CO2 and O2) on the induction events in cyanobacteria (P700 photooxidation, generation of transmembrane ApH), which strongly depends on pre-illumination conditions (aerobic or anaerobic atmosphere). It has been shown that the variation of C02 concentration in the cell interior may noticeably affect the kinetics of electron transport, acidification of lumen, and ATP synthesis. The contribution of alternative pathways of the electron transport (cyclic electron transport around PS1 and outflow of electrons to O2) to the operation of cyanobacteria photosynthetic apparatus was analyzed. At the initial stage of induction period, cyclic electron fluxes around PS1 ("short" and "long" pathways) contribute substantially to photosynthetic electron transport. These fluxes, however, attenuate with the light-induced activation of the Calvin-Benson cycle reactions. In the meantime, efflux of electrons from PS 1 to O2 (or to another metabolic chains) increases with the oxygen accumulation in the medium. Effects of ferredoxin oxidation by hydrogenase, catalyzing formation of H2, on the kinetics of P700 photooxidation and distribution of electron fluxes on the acceptor side of PS 1 has been modeled.

Original languageEnglish
Pages (from-to)110-128
Number of pages19
JournalBiologicheskie Membrany
Volume31
Issue number2
DOIs
Publication statusPublished - 2014
Externally publishedYes

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Cyanobacteria
Electron Transport
Electrons
Photosynthesis
Atmosphere
Hydrogenase
Light
Lighting
Computer Simulation
Oxidation-Reduction
Protons
Theoretical Models
Adenosine Triphosphate
Gases
Oxygen

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Modeling of the photosynthetic electron transport regulation in cyanobacteria. / Vershubskii, A. V.; Mishanin, V. I.; Tikhonov, A. N.

In: Biologicheskie Membrany, Vol. 31, No. 2, 2014, p. 110-128.

Research output: Contribution to journalArticle

Vershubskii, A. V. ; Mishanin, V. I. ; Tikhonov, A. N. / Modeling of the photosynthetic electron transport regulation in cyanobacteria. In: Biologicheskie Membrany. 2014 ; Vol. 31, No. 2. pp. 110-128.
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