Molecular architecture of the nucleoprotein C-terminal domain from the Ebola and Marburg viruses

Laura E. Baker, Jeffrey F. Ellena, Katarzyna B. Handing, Urszula Derewenda, Darkhan Utepbergenov, Daniel A. Engel, Zygmunt S. Derewenda

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The Filoviridae family of negative-sense, single-stranded RNA (ssRNA) viruses is comprised of two species of Marburgvirus (MARV and RAVV) and five species of Ebolavirus, i.e. Zaire (EBOV), Reston (RESTV), Sudan (SUDV), Taï Forest (TAFV) and Bundibugyo (BDBV). In each of these viruses the ssRNA encodes seven distinct proteins. One of them, the nucleoprotein (NP), is the most abundant viral protein in the infected cell and within the viral nucleocapsid. It is tightly associated with the viral RNA in the nucleocapsid, and during the lifecycle of the virus is essential for transcription, RNA replication, genome packaging and nucleocapsid assembly prior to membrane encapsulation. The structure of the unique C-terminal globular domain of the NP from EBOV has recently been determined and shown to be structurally unrelated to any other known protein [Dziubańska et al. (2014), Acta Cryst.D70, 2420–2429]. In this paper, a study of the C-terminal domains from the NP from the remaining four species of Ebolavirus, as well as from the MARV strain of Marburgvirus,is reported. As expected, the crystal structures of the BDBV and TAFV proteins show high structural similarity to that from EBOV, while the MARV protein behaves like a molten globule with a core residual structure that is significantly different from that of the EBOV protein.

Original languageEnglish
Pages (from-to)49-58
Number of pages10
JournalActa Crystallographica Section D: Structural Biology
Volume72
Issue number1
DOIs
Publication statusPublished - 2016

Keywords

  • Ebolavirus
  • Filoviridae
  • Marburgvirus
  • Nucleoprotein
  • Viral proteins

ASJC Scopus subject areas

  • Structural Biology

Fingerprint Dive into the research topics of 'Molecular architecture of the nucleoprotein C-terminal domain from the Ebola and Marburg viruses'. Together they form a unique fingerprint.

Cite this