Молекулярно-генетическая идентификация популяций Pinus sylvestris L. и Larix sibirica Ledeb. в Пермском крае с использованием SNP-маркеров

Translated title of the contribution: Molecular Genetic Identification of Populations of Pinus sylvestris L. and Larix sibirica Ledeb. in Perm Krai Using SNP-markers

Nikita Chertov, N Pystogova, E Malyshkina, Yu Nechaeva, Svetlana Boronnikova, Ruslan Kalendar

Research output: Contribution to journalArticlepeer-review

Abstract

For the selection of identification SNP markers and identification at the population level, 5 populations of Scots pine (Pinus sylvestris L., Pinaceae) and 5 populations of the western race of Siberian larch (Larix sibirica Ledeb., Pinaceae) in the Perm Krai were studied. To determine nucleotide sequences and select identification SNP markers in 10 populations of two woody plant species of the Perm Krai, 14 pairs of nuclear and 3 pairs of chloroplast loci of P. sylvestris, as well as ten pairs of primers to 10 loci of potentially adaptively significant genes of L. sibirica were tested. Sequencing of 3 nuclear loci and 3 loci of chloroplast DNA of P. sylvestris, as well as 6 loci of nuclear adaptively significant genes of L. sibirica were sequenced. In the sequenced sequences of two plant species, 59 SNP markers were detected. Of these, 11 identification SNP markers were established, with a sufficiently high frequency of occurrence (≥0.5), suitable for identifying populations of two coniferous plant species.
Translated title of the contributionMolecular Genetic Identification of Populations of Pinus sylvestris L. and Larix sibirica Ledeb. in Perm Krai Using SNP-markers
Original languageRussian
Pages (from-to)14-22
JournalBulletin of Science and Practice
Volume6
Issue number12
DOIs
Publication statusPublished - Dec 16 2020

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