Molecular hybridization probes prepared with 4-aminooxybutylamine

Vyacheslav A. Adarichev, Sergey M. Kalachikov, Anna V. Kiseliova, Grigory M. Dymshits

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A versatile method is described for preparing nonradioactive DNA probes for molecular hybridization. The method is based on the transamination reaction of double-stranded DNA with 4-aminooxybutylamine (ABA). To optimize the procedure for obtaining stable and sensitive hybridization probes, time of modification, pH, and reaction temperature were varied. The optimal reaction conditions allowed the preparation of nonradioactive-labeled DNA probes that met demands for optimal length, modification degree, stability, and sensitivity. The use of 4-aminooxybutylamine as a bifunctional reagent for DNA modification allowed the possibility of choosing an appropriate reporter group: biotin or one of the fluorochromes. For probes carrying biotin, a high specificity and high sensitivity detection limit of 1 pg of target DNA were demonstrated. In addition, the applicability of probes carrying fluorochromes for multicolor direct fluorescence in situ hybridization was described.

Original languageEnglish
Pages (from-to)671-675
Number of pages5
JournalBioconjugate Chemistry
Volume9
Issue number6
DOIs
Publication statusPublished - Nov 1998
Externally publishedYes

Fingerprint

Molecular Probes
DNA
DNA Probes
Biotin
Fluorescent Dyes
Cross-Linking Reagents
Fluorescence In Situ Hybridization
Limit of Detection
Fluorescence
Sensitivity and Specificity
Temperature
4-aminooxybutylamine

ASJC Scopus subject areas

  • Chemistry(all)
  • Organic Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Adarichev, V. A., Kalachikov, S. M., Kiseliova, A. V., & Dymshits, G. M. (1998). Molecular hybridization probes prepared with 4-aminooxybutylamine. Bioconjugate Chemistry, 9(6), 671-675. https://doi.org/10.1021/bc980007u

Molecular hybridization probes prepared with 4-aminooxybutylamine. / Adarichev, Vyacheslav A.; Kalachikov, Sergey M.; Kiseliova, Anna V.; Dymshits, Grigory M.

In: Bioconjugate Chemistry, Vol. 9, No. 6, 11.1998, p. 671-675.

Research output: Contribution to journalArticle

Adarichev, VA, Kalachikov, SM, Kiseliova, AV & Dymshits, GM 1998, 'Molecular hybridization probes prepared with 4-aminooxybutylamine', Bioconjugate Chemistry, vol. 9, no. 6, pp. 671-675. https://doi.org/10.1021/bc980007u
Adarichev VA, Kalachikov SM, Kiseliova AV, Dymshits GM. Molecular hybridization probes prepared with 4-aminooxybutylamine. Bioconjugate Chemistry. 1998 Nov;9(6):671-675. https://doi.org/10.1021/bc980007u
Adarichev, Vyacheslav A. ; Kalachikov, Sergey M. ; Kiseliova, Anna V. ; Dymshits, Grigory M. / Molecular hybridization probes prepared with 4-aminooxybutylamine. In: Bioconjugate Chemistry. 1998 ; Vol. 9, No. 6. pp. 671-675.
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