Molecular proximity of cystic fibrosis transmembrane conductance regulator and epithelial sodium channel assessed by fluorescence resonance energy transfer

Bakhrom K. Berdiev, Estelle Cormet-Boyaka, Albert Tousson, Yawar J. Qadri, Henderika M.J. Oosterveld-Hut, Jeong S. Hong, Atricia A. Gonzales, Cathy M. Fuller, Eric J. Sorscher, Gergely L. Lukacs, Dale J. Benos

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

We present the evidence for a direct physical association of cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC), two major ion channels implicated in the pathophysiology of cystic fibrosis, a devastating inherited disease. We employed fluorescence resonance energy transfer, a distance-dependent imaging technique with capability to detect molecular complexes with near angstrom resolution, to estimate the proximity of CFTR and ENaC, an essential variable for possible physical interaction to occur. Fluorescence resonance energy transfer studies were complemented with a classic biochemical approach: coimmunoprecipitation. Our results place CFTR and ENaC within reach of each other, suggestive of a direct interaction between these two proteins.

Original languageEnglish
Pages (from-to)36481-36488
Number of pages8
JournalJournal of Biological Chemistry
Volume282
Issue number50
DOIs
Publication statusPublished - Dec 14 2007

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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