Abstract
We present the evidence for a direct physical association of cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC), two major ion channels implicated in the pathophysiology of cystic fibrosis, a devastating inherited disease. We employed fluorescence resonance energy transfer, a distance-dependent imaging technique with capability to detect molecular complexes with near angstrom resolution, to estimate the proximity of CFTR and ENaC, an essential variable for possible physical interaction to occur. Fluorescence resonance energy transfer studies were complemented with a classic biochemical approach: coimmunoprecipitation. Our results place CFTR and ENaC within reach of each other, suggestive of a direct interaction between these two proteins.
| Original language | English |
|---|---|
| Pages (from-to) | 36481-36488 |
| Number of pages | 8 |
| Journal | Journal of Biological Chemistry |
| Volume | 282 |
| Issue number | 50 |
| DOIs | |
| Publication status | Published - Dec 14 2007 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Molecular proximity of cystic fibrosis transmembrane conductance regulator and epithelial sodium channel assessed by fluorescence resonance energy transfer. / Berdiev, Bakhrom K.; Cormet-Boyaka, Estelle; Tousson, Albert; Qadri, Yawar J.; Oosterveld-Hut, Henderika M J; Hong, Jeong S.; Gonzales, Atricia A.; Fuller, Cathy M.; Sorscher, Eric J.; Lukacs, Gergely L.; Benos, Dale J.
In: Journal of Biological Chemistry, Vol. 282, No. 50, 14.12.2007, p. 36481-36488.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Molecular proximity of cystic fibrosis transmembrane conductance regulator and epithelial sodium channel assessed by fluorescence resonance energy transfer
AU - Berdiev, Bakhrom K.
AU - Cormet-Boyaka, Estelle
AU - Tousson, Albert
AU - Qadri, Yawar J.
AU - Oosterveld-Hut, Henderika M J
AU - Hong, Jeong S.
AU - Gonzales, Atricia A.
AU - Fuller, Cathy M.
AU - Sorscher, Eric J.
AU - Lukacs, Gergely L.
AU - Benos, Dale J.
PY - 2007/12/14
Y1 - 2007/12/14
N2 - We present the evidence for a direct physical association of cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC), two major ion channels implicated in the pathophysiology of cystic fibrosis, a devastating inherited disease. We employed fluorescence resonance energy transfer, a distance-dependent imaging technique with capability to detect molecular complexes with near angstrom resolution, to estimate the proximity of CFTR and ENaC, an essential variable for possible physical interaction to occur. Fluorescence resonance energy transfer studies were complemented with a classic biochemical approach: coimmunoprecipitation. Our results place CFTR and ENaC within reach of each other, suggestive of a direct interaction between these two proteins.
AB - We present the evidence for a direct physical association of cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC), two major ion channels implicated in the pathophysiology of cystic fibrosis, a devastating inherited disease. We employed fluorescence resonance energy transfer, a distance-dependent imaging technique with capability to detect molecular complexes with near angstrom resolution, to estimate the proximity of CFTR and ENaC, an essential variable for possible physical interaction to occur. Fluorescence resonance energy transfer studies were complemented with a classic biochemical approach: coimmunoprecipitation. Our results place CFTR and ENaC within reach of each other, suggestive of a direct interaction between these two proteins.
UR - http://www.scopus.com/inward/record.url?scp=37549035927&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=37549035927&partnerID=8YFLogxK
U2 - 10.1074/jbc.M708089200
DO - 10.1074/jbc.M708089200
M3 - Article
VL - 282
SP - 36481
EP - 36488
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 50
ER -