Molecular proximity of cystic fibrosis transmembrane conductance regulator and epithelial sodium channel assessed by fluorescence resonance energy transfer

Bakhrom K. Berdiev, Estelle Cormet-Boyaka, Albert Tousson, Yawar J. Qadri, Henderika M J Oosterveld-Hut, Jeong S. Hong, Atricia A. Gonzales, Cathy M. Fuller, Eric J. Sorscher, Gergely L. Lukacs, Dale J. Benos

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We present the evidence for a direct physical association of cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC), two major ion channels implicated in the pathophysiology of cystic fibrosis, a devastating inherited disease. We employed fluorescence resonance energy transfer, a distance-dependent imaging technique with capability to detect molecular complexes with near angstrom resolution, to estimate the proximity of CFTR and ENaC, an essential variable for possible physical interaction to occur. Fluorescence resonance energy transfer studies were complemented with a classic biochemical approach: coimmunoprecipitation. Our results place CFTR and ENaC within reach of each other, suggestive of a direct interaction between these two proteins.

Original languageEnglish
Pages (from-to)36481-36488
Number of pages8
JournalJournal of Biological Chemistry
Volume282
Issue number50
DOIs
Publication statusPublished - Dec 14 2007
Externally publishedYes

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Epithelial Sodium Channels
Cystic Fibrosis Transmembrane Conductance Regulator
Fluorescence Resonance Energy Transfer
Ion Channels
Cystic Fibrosis
Imaging techniques
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Berdiev, B. K., Cormet-Boyaka, E., Tousson, A., Qadri, Y. J., Oosterveld-Hut, H. M. J., Hong, J. S., ... Benos, D. J. (2007). Molecular proximity of cystic fibrosis transmembrane conductance regulator and epithelial sodium channel assessed by fluorescence resonance energy transfer. Journal of Biological Chemistry, 282(50), 36481-36488. https://doi.org/10.1074/jbc.M708089200

Molecular proximity of cystic fibrosis transmembrane conductance regulator and epithelial sodium channel assessed by fluorescence resonance energy transfer. / Berdiev, Bakhrom K.; Cormet-Boyaka, Estelle; Tousson, Albert; Qadri, Yawar J.; Oosterveld-Hut, Henderika M J; Hong, Jeong S.; Gonzales, Atricia A.; Fuller, Cathy M.; Sorscher, Eric J.; Lukacs, Gergely L.; Benos, Dale J.

In: Journal of Biological Chemistry, Vol. 282, No. 50, 14.12.2007, p. 36481-36488.

Research output: Contribution to journalArticle

Berdiev, BK, Cormet-Boyaka, E, Tousson, A, Qadri, YJ, Oosterveld-Hut, HMJ, Hong, JS, Gonzales, AA, Fuller, CM, Sorscher, EJ, Lukacs, GL & Benos, DJ 2007, 'Molecular proximity of cystic fibrosis transmembrane conductance regulator and epithelial sodium channel assessed by fluorescence resonance energy transfer', Journal of Biological Chemistry, vol. 282, no. 50, pp. 36481-36488. https://doi.org/10.1074/jbc.M708089200
Berdiev, Bakhrom K. ; Cormet-Boyaka, Estelle ; Tousson, Albert ; Qadri, Yawar J. ; Oosterveld-Hut, Henderika M J ; Hong, Jeong S. ; Gonzales, Atricia A. ; Fuller, Cathy M. ; Sorscher, Eric J. ; Lukacs, Gergely L. ; Benos, Dale J. / Molecular proximity of cystic fibrosis transmembrane conductance regulator and epithelial sodium channel assessed by fluorescence resonance energy transfer. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 50. pp. 36481-36488.
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AU - Oosterveld-Hut, Henderika M J

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