Morphological changes of Ascaris spp. eggs during their development outside the host

Ligia M. Cruz, Michael Allanson, Boo Kwa, Azliyati Azizan, Ricardo Izurieta

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Information on the infective stage of Ascaris lumbricoides and the pathology caused by the parasite is widely available in the literature. However, information about early embryonic development of A. lumbricoides and its life cycle outside the host is limited. The purpose of this study was to describe the morphological changes within the developing embryo during incubation in vitro at 28 C, as well as to explore differences in egg viability during incubation. Ascaris suum eggs (4,000 eggs/ml), used as a model for A. lumbricoides, were placed for incubation in 0.1N H 2SO 4 at 28 C in the dark for 21 days. Every day, sub-samples of approximately 100 A. suum eggs were taken from the incubation solution for microscopic evaluation. Development, morphological changes, and viability of the first 40 eggs were observed and documented with photos. During this study, 12 stages were identified in the developing embryo by standard microscopy, 2 of which had not been previously reported. By the end of the first wk, most developing embryos observed were in the late-morula stage (72.5%). On day 14 of incubation, 90% had developed to larva-1 stage, and by day 21, 100% had developed to larva-2 stage. No significant differences were found in the viability recorded in a continuum from day 5 to day 21 of incubation (chi-square, P > 0.05). The result of this study complements and expands the stages of development of Ascaris spp. outside the host previously reported in the literature. It also suggests the potential use of early stages of development of the nematode to determine viability and safety of sewage sludge, wastewater, or compost after treatment recommended by USEPA.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalJournal of Parasitology
Volume98
Issue number1
DOIs
Publication statusPublished - Feb 2012
Externally publishedYes

Fingerprint

Ascaris
Ascaris lumbricoides
Eggs
incubation
viability
egg
Ascaris suum
embryo (animal)
Embryonic Structures
Sewage
Larva
embryo
Morula
United States Environmental Protection Agency
larvae
morula
sewage sludge
Waste Water
Life Cycle Stages
composts

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Morphological changes of Ascaris spp. eggs during their development outside the host. / Cruz, Ligia M.; Allanson, Michael; Kwa, Boo; Azizan, Azliyati; Izurieta, Ricardo.

In: Journal of Parasitology, Vol. 98, No. 1, 02.2012, p. 63-68.

Research output: Contribution to journalArticle

Cruz, Ligia M. ; Allanson, Michael ; Kwa, Boo ; Azizan, Azliyati ; Izurieta, Ricardo. / Morphological changes of Ascaris spp. eggs during their development outside the host. In: Journal of Parasitology. 2012 ; Vol. 98, No. 1. pp. 63-68.
@article{659eae8c208341e4991b766132ed1d20,
title = "Morphological changes of Ascaris spp. eggs during their development outside the host",
abstract = "Information on the infective stage of Ascaris lumbricoides and the pathology caused by the parasite is widely available in the literature. However, information about early embryonic development of A. lumbricoides and its life cycle outside the host is limited. The purpose of this study was to describe the morphological changes within the developing embryo during incubation in vitro at 28 C, as well as to explore differences in egg viability during incubation. Ascaris suum eggs (4,000 eggs/ml), used as a model for A. lumbricoides, were placed for incubation in 0.1N H 2SO 4 at 28 C in the dark for 21 days. Every day, sub-samples of approximately 100 A. suum eggs were taken from the incubation solution for microscopic evaluation. Development, morphological changes, and viability of the first 40 eggs were observed and documented with photos. During this study, 12 stages were identified in the developing embryo by standard microscopy, 2 of which had not been previously reported. By the end of the first wk, most developing embryos observed were in the late-morula stage (72.5{\%}). On day 14 of incubation, 90{\%} had developed to larva-1 stage, and by day 21, 100{\%} had developed to larva-2 stage. No significant differences were found in the viability recorded in a continuum from day 5 to day 21 of incubation (chi-square, P > 0.05). The result of this study complements and expands the stages of development of Ascaris spp. outside the host previously reported in the literature. It also suggests the potential use of early stages of development of the nematode to determine viability and safety of sewage sludge, wastewater, or compost after treatment recommended by USEPA.",
author = "Cruz, {Ligia M.} and Michael Allanson and Boo Kwa and Azliyati Azizan and Ricardo Izurieta",
year = "2012",
month = "2",
doi = "10.1645/GE-2821.1",
language = "English",
volume = "98",
pages = "63--68",
journal = "Journal of Parasitology",
issn = "0022-3395",
publisher = "American Society of Parasitologists",
number = "1",

}

TY - JOUR

T1 - Morphological changes of Ascaris spp. eggs during their development outside the host

AU - Cruz, Ligia M.

AU - Allanson, Michael

AU - Kwa, Boo

AU - Azizan, Azliyati

AU - Izurieta, Ricardo

PY - 2012/2

Y1 - 2012/2

N2 - Information on the infective stage of Ascaris lumbricoides and the pathology caused by the parasite is widely available in the literature. However, information about early embryonic development of A. lumbricoides and its life cycle outside the host is limited. The purpose of this study was to describe the morphological changes within the developing embryo during incubation in vitro at 28 C, as well as to explore differences in egg viability during incubation. Ascaris suum eggs (4,000 eggs/ml), used as a model for A. lumbricoides, were placed for incubation in 0.1N H 2SO 4 at 28 C in the dark for 21 days. Every day, sub-samples of approximately 100 A. suum eggs were taken from the incubation solution for microscopic evaluation. Development, morphological changes, and viability of the first 40 eggs were observed and documented with photos. During this study, 12 stages were identified in the developing embryo by standard microscopy, 2 of which had not been previously reported. By the end of the first wk, most developing embryos observed were in the late-morula stage (72.5%). On day 14 of incubation, 90% had developed to larva-1 stage, and by day 21, 100% had developed to larva-2 stage. No significant differences were found in the viability recorded in a continuum from day 5 to day 21 of incubation (chi-square, P > 0.05). The result of this study complements and expands the stages of development of Ascaris spp. outside the host previously reported in the literature. It also suggests the potential use of early stages of development of the nematode to determine viability and safety of sewage sludge, wastewater, or compost after treatment recommended by USEPA.

AB - Information on the infective stage of Ascaris lumbricoides and the pathology caused by the parasite is widely available in the literature. However, information about early embryonic development of A. lumbricoides and its life cycle outside the host is limited. The purpose of this study was to describe the morphological changes within the developing embryo during incubation in vitro at 28 C, as well as to explore differences in egg viability during incubation. Ascaris suum eggs (4,000 eggs/ml), used as a model for A. lumbricoides, were placed for incubation in 0.1N H 2SO 4 at 28 C in the dark for 21 days. Every day, sub-samples of approximately 100 A. suum eggs were taken from the incubation solution for microscopic evaluation. Development, morphological changes, and viability of the first 40 eggs were observed and documented with photos. During this study, 12 stages were identified in the developing embryo by standard microscopy, 2 of which had not been previously reported. By the end of the first wk, most developing embryos observed were in the late-morula stage (72.5%). On day 14 of incubation, 90% had developed to larva-1 stage, and by day 21, 100% had developed to larva-2 stage. No significant differences were found in the viability recorded in a continuum from day 5 to day 21 of incubation (chi-square, P > 0.05). The result of this study complements and expands the stages of development of Ascaris spp. outside the host previously reported in the literature. It also suggests the potential use of early stages of development of the nematode to determine viability and safety of sewage sludge, wastewater, or compost after treatment recommended by USEPA.

UR - http://www.scopus.com/inward/record.url?scp=84857818235&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857818235&partnerID=8YFLogxK

U2 - 10.1645/GE-2821.1

DO - 10.1645/GE-2821.1

M3 - Article

VL - 98

SP - 63

EP - 68

JO - Journal of Parasitology

JF - Journal of Parasitology

SN - 0022-3395

IS - 1

ER -