Morphological interactions of human first trimester placental villi co-cultured with decidual explants

M. O. Babawale, S. Van Noorden, M. Pignatelli, G. W H Stamp, M. G. Elder, M. H F Sullivan

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Abnormalities of pregnancy such as pre-eclampsia and intrauterine growth retardation are characterized by shallow trophoblastic invasion of the placental bed, the precise molecular pathophysiology of which remains to be fully elucidated. An in-vitro model involving a co-culture of first trimester placental villi and decidua parietalis explants (of 8-12 weeks gestation) was developed and used to characterize the migration and local invasion of trophoblast cells. Trophoblast proliferation (confirmed by Ki-67 immunostaining), differentiation and loose attachment of placental villi to the underlying decidual epithelium or stroma occurred, within the first 24 h of co-culture. This was followed by erosion of the syncytial layer of the placental villi and commencement of a progressive cytotrophoblast invasion after 48 h of co-culture, which continued until 120 h, when the experiments were terminated. E-cadherin was expressed at the interfaces between trophoblast cells within the villi, but expression of this adhesion molecule seemed to be down-regulated in the invasive trophoblast cells. Our results suggest that the model could be useful in investigating the factors that control early human placentation and the fete-maternal interface.

Original languageEnglish
Pages (from-to)444-450
Number of pages7
JournalHuman Reproduction
Volume11
Issue number2
Publication statusPublished - Feb 1996
Externally publishedYes

Fingerprint

Chorionic Villi
Trophoblasts
First Pregnancy Trimester
Coculture Techniques
Decidua
Placentation
Pregnancy
Fetal Growth Retardation
Cadherins
Pre-Eclampsia
Epithelium
Mothers

Keywords

  • Decidua
  • E-cadherin
  • Ki-67
  • Placentation
  • Trophoblast

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Babawale, M. O., Van Noorden, S., Pignatelli, M., Stamp, G. W. H., Elder, M. G., & Sullivan, M. H. F. (1996). Morphological interactions of human first trimester placental villi co-cultured with decidual explants. Human Reproduction, 11(2), 444-450.

Morphological interactions of human first trimester placental villi co-cultured with decidual explants. / Babawale, M. O.; Van Noorden, S.; Pignatelli, M.; Stamp, G. W H; Elder, M. G.; Sullivan, M. H F.

In: Human Reproduction, Vol. 11, No. 2, 02.1996, p. 444-450.

Research output: Contribution to journalArticle

Babawale, MO, Van Noorden, S, Pignatelli, M, Stamp, GWH, Elder, MG & Sullivan, MHF 1996, 'Morphological interactions of human first trimester placental villi co-cultured with decidual explants', Human Reproduction, vol. 11, no. 2, pp. 444-450.
Babawale MO, Van Noorden S, Pignatelli M, Stamp GWH, Elder MG, Sullivan MHF. Morphological interactions of human first trimester placental villi co-cultured with decidual explants. Human Reproduction. 1996 Feb;11(2):444-450.
Babawale, M. O. ; Van Noorden, S. ; Pignatelli, M. ; Stamp, G. W H ; Elder, M. G. ; Sullivan, M. H F. / Morphological interactions of human first trimester placental villi co-cultured with decidual explants. In: Human Reproduction. 1996 ; Vol. 11, No. 2. pp. 444-450.
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