Abstract
Abnormalities of pregnancy such as pre-eclampsia and intrauterine growth retardation are characterized by shallow trophoblastic invasion of the placental bed, the precise molecular pathophysiology of which remains to be fully elucidated. An in-vitro model involving a co-culture of first trimester placental villi and decidua parietalis explants (of 8-12 weeks gestation) was developed and used to characterize the migration and local invasion of trophoblast cells. Trophoblast proliferation (confirmed by Ki-67 immunostaining), differentiation and loose attachment of placental villi to the underlying decidual epithelium or stroma occurred, within the first 24 h of co-culture. This was followed by erosion of the syncytial layer of the placental villi and commencement of a progressive cytotrophoblast invasion after 48 h of co-culture, which continued until 120 h, when the experiments were terminated. E-cadherin was expressed at the interfaces between trophoblast cells within the villi, but expression of this adhesion molecule seemed to be down-regulated in the invasive trophoblast cells. Our results suggest that the model could be useful in investigating the factors that control early human placentation and the fete-maternal interface.
| Original language | English |
|---|---|
| Pages (from-to) | 444-450 |
| Number of pages | 7 |
| Journal | Human Reproduction |
| Volume | 11 |
| Issue number | 2 |
| Publication status | Published - Feb 1996 |
| Externally published | Yes |
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Keywords
- Decidua
- E-cadherin
- Ki-67
- Placentation
- Trophoblast
ASJC Scopus subject areas
- Physiology
- Developmental Biology
- Obstetrics and Gynaecology
- Reproductive Medicine
Cite this
Morphological interactions of human first trimester placental villi co-cultured with decidual explants. / Babawale, M. O.; Van Noorden, S.; Pignatelli, M.; Stamp, G. W H; Elder, M. G.; Sullivan, M. H F.
In: Human Reproduction, Vol. 11, No. 2, 02.1996, p. 444-450.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Morphological interactions of human first trimester placental villi co-cultured with decidual explants
AU - Babawale, M. O.
AU - Van Noorden, S.
AU - Pignatelli, M.
AU - Stamp, G. W H
AU - Elder, M. G.
AU - Sullivan, M. H F
PY - 1996/2
Y1 - 1996/2
N2 - Abnormalities of pregnancy such as pre-eclampsia and intrauterine growth retardation are characterized by shallow trophoblastic invasion of the placental bed, the precise molecular pathophysiology of which remains to be fully elucidated. An in-vitro model involving a co-culture of first trimester placental villi and decidua parietalis explants (of 8-12 weeks gestation) was developed and used to characterize the migration and local invasion of trophoblast cells. Trophoblast proliferation (confirmed by Ki-67 immunostaining), differentiation and loose attachment of placental villi to the underlying decidual epithelium or stroma occurred, within the first 24 h of co-culture. This was followed by erosion of the syncytial layer of the placental villi and commencement of a progressive cytotrophoblast invasion after 48 h of co-culture, which continued until 120 h, when the experiments were terminated. E-cadherin was expressed at the interfaces between trophoblast cells within the villi, but expression of this adhesion molecule seemed to be down-regulated in the invasive trophoblast cells. Our results suggest that the model could be useful in investigating the factors that control early human placentation and the fete-maternal interface.
AB - Abnormalities of pregnancy such as pre-eclampsia and intrauterine growth retardation are characterized by shallow trophoblastic invasion of the placental bed, the precise molecular pathophysiology of which remains to be fully elucidated. An in-vitro model involving a co-culture of first trimester placental villi and decidua parietalis explants (of 8-12 weeks gestation) was developed and used to characterize the migration and local invasion of trophoblast cells. Trophoblast proliferation (confirmed by Ki-67 immunostaining), differentiation and loose attachment of placental villi to the underlying decidual epithelium or stroma occurred, within the first 24 h of co-culture. This was followed by erosion of the syncytial layer of the placental villi and commencement of a progressive cytotrophoblast invasion after 48 h of co-culture, which continued until 120 h, when the experiments were terminated. E-cadherin was expressed at the interfaces between trophoblast cells within the villi, but expression of this adhesion molecule seemed to be down-regulated in the invasive trophoblast cells. Our results suggest that the model could be useful in investigating the factors that control early human placentation and the fete-maternal interface.
KW - Decidua
KW - E-cadherin
KW - Ki-67
KW - Placentation
KW - Trophoblast
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UR - http://www.scopus.com/inward/citedby.url?scp=0029935346&partnerID=8YFLogxK
M3 - Article
C2 - 8671240
AN - SCOPUS:0029935346
VL - 11
SP - 444
EP - 450
JO - Human Reproduction
JF - Human Reproduction
SN - 0268-1161
IS - 2
ER -