Morphological interactions of human first trimester placental villi co-cultured with decidual explants

M. O. Babawale; S. Van Noorden; M. Pignatelli; G. W.H. Stamp; M. G. Elder; M. H.F. Sullivan

doi:10.1093/HUMREP/11.2.444

Morphological interactions of human first trimester placental villi co-cultured with decidual explants

M. O. Babawale
, S. Van Noorden
, M. Pignatelli
, G. W.H. Stamp
, M. G. Elder
, M. H.F. Sullivan

Hammersmith Hospital

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Abnormalities of pregnancy such as pre-eclampsia and intrauterine growth retardation are characterized by shallow trophoblastic invasion of the placental bed, the precise molecular pathophysiology of which remains to be fully elucidated. An in-vitro model involving a co-culture of first trimester placental villi and decidua parietalis explants (of 8-12 weeks gestation) was developed and used to characterize the migration and local invasion of trophoblast cells. Trophoblast proliferation (confirmed by Ki-67 immunostaining), differentiation and loose attachment of placental villi to the underlying decidual epithelium or stroma occurred, within the first 24 h of co-culture. This was followed by erosion of the syncytial layer of the placental villi and commencement of a progressive cytotrophoblast invasion after 48 h of co-culture, which continued until 120 h, when the experiments were terminated. E-cadherin was expressed at the interfaces between trophoblast cells within the villi, but expression of this adhesion molecule seemed to be down-regulated in the invasive trophoblast cells. Our results suggest that the model could be useful in investigating the factors that control early human placentation and the fete-maternal interface.

Original language	English
Pages (from-to)	444-450
Number of pages	7
Journal	Human Reproduction
Volume	11
Issue number	2
DOIs	https://doi.org/10.1093/HUMREP/11.2.444
Publication status	Published - Feb 1996
Externally published	Yes

Funding

We arc grateful to Professor N. Wright of the Department of Histopathology for the use of facilities in his department; we would also like to thank Professor J.S.W. Wigglesworth of the same department for reading the manuscript and for his helpful comments. We also extend our gratitude to Professor M. Takeichi (Kyoto University, Japan) for the gift of E-cadherin antibody. This work was supported by medical research grants from The Sir Halley Stewart Trust (Cambridge, UK) to M.O.B. and The Robert McAlpine Foundation to M.H.F.S., for which the authors are very grateful.

Keywords

Decidua
E-cadherin
Ki-67
Placentation
Trophoblast

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynaecology

Access to Document

10.1093/HUMREP/11.2.444

Cite this

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title = "Morphological interactions of human first trimester placental villi co-cultured with decidual explants",

abstract = "Abnormalities of pregnancy such as pre-eclampsia and intrauterine growth retardation are characterized by shallow trophoblastic invasion of the placental bed, the precise molecular pathophysiology of which remains to be fully elucidated. An in-vitro model involving a co-culture of first trimester placental villi and decidua parietalis explants (of 8-12 weeks gestation) was developed and used to characterize the migration and local invasion of trophoblast cells. Trophoblast proliferation (confirmed by Ki-67 immunostaining), differentiation and loose attachment of placental villi to the underlying decidual epithelium or stroma occurred, within the first 24 h of co-culture. This was followed by erosion of the syncytial layer of the placental villi and commencement of a progressive cytotrophoblast invasion after 48 h of co-culture, which continued until 120 h, when the experiments were terminated. E-cadherin was expressed at the interfaces between trophoblast cells within the villi, but expression of this adhesion molecule seemed to be down-regulated in the invasive trophoblast cells. Our results suggest that the model could be useful in investigating the factors that control early human placentation and the fete-maternal interface.",

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note = "Funding Information: We arc grateful to Professor N. Wright of the Department of Histopathology for the use of facilities in his department; we would also like to thank Professor J.S.W. Wigglesworth of the same department for reading the manuscript and for his helpful comments. We also extend our gratitude to Professor M. Takeichi (Kyoto University, Japan) for the gift of E-cadherin antibody. This work was supported by medical research grants from The Sir Halley Stewart Trust (Cambridge, UK) to M.O.B. and The Robert McAlpine Foundation to M.H.F.S., for which the authors are very grateful.",

year = "1996",

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doi = "10.1093/HUMREP/11.2.444",

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AU - Van Noorden, S.

AU - Pignatelli, M.

AU - Stamp, G. W.H.

AU - Elder, M. G.

AU - Sullivan, M. H.F.

N1 - Funding Information: We arc grateful to Professor N. Wright of the Department of Histopathology for the use of facilities in his department; we would also like to thank Professor J.S.W. Wigglesworth of the same department for reading the manuscript and for his helpful comments. We also extend our gratitude to Professor M. Takeichi (Kyoto University, Japan) for the gift of E-cadherin antibody. This work was supported by medical research grants from The Sir Halley Stewart Trust (Cambridge, UK) to M.O.B. and The Robert McAlpine Foundation to M.H.F.S., for which the authors are very grateful.

PY - 1996/2

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N2 - Abnormalities of pregnancy such as pre-eclampsia and intrauterine growth retardation are characterized by shallow trophoblastic invasion of the placental bed, the precise molecular pathophysiology of which remains to be fully elucidated. An in-vitro model involving a co-culture of first trimester placental villi and decidua parietalis explants (of 8-12 weeks gestation) was developed and used to characterize the migration and local invasion of trophoblast cells. Trophoblast proliferation (confirmed by Ki-67 immunostaining), differentiation and loose attachment of placental villi to the underlying decidual epithelium or stroma occurred, within the first 24 h of co-culture. This was followed by erosion of the syncytial layer of the placental villi and commencement of a progressive cytotrophoblast invasion after 48 h of co-culture, which continued until 120 h, when the experiments were terminated. E-cadherin was expressed at the interfaces between trophoblast cells within the villi, but expression of this adhesion molecule seemed to be down-regulated in the invasive trophoblast cells. Our results suggest that the model could be useful in investigating the factors that control early human placentation and the fete-maternal interface.

AB - Abnormalities of pregnancy such as pre-eclampsia and intrauterine growth retardation are characterized by shallow trophoblastic invasion of the placental bed, the precise molecular pathophysiology of which remains to be fully elucidated. An in-vitro model involving a co-culture of first trimester placental villi and decidua parietalis explants (of 8-12 weeks gestation) was developed and used to characterize the migration and local invasion of trophoblast cells. Trophoblast proliferation (confirmed by Ki-67 immunostaining), differentiation and loose attachment of placental villi to the underlying decidual epithelium or stroma occurred, within the first 24 h of co-culture. This was followed by erosion of the syncytial layer of the placental villi and commencement of a progressive cytotrophoblast invasion after 48 h of co-culture, which continued until 120 h, when the experiments were terminated. E-cadherin was expressed at the interfaces between trophoblast cells within the villi, but expression of this adhesion molecule seemed to be down-regulated in the invasive trophoblast cells. Our results suggest that the model could be useful in investigating the factors that control early human placentation and the fete-maternal interface.

KW - Decidua

KW - E-cadherin

KW - Ki-67

KW - Placentation

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Morphological interactions of human first trimester placental villi co-cultured with decidual explants

Abstract

Funding

Keywords

ASJC Scopus subject areas

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