Morphological responses of the rabbit testis to ischemic/reperfusion injury due to torsion

J. T. Anim, E. O. Kehinde, Asa Prasad, R. Varghese

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective: To investigate the morphological effects of free radical injury on spermatogenic cells in both testes of the rabbit experimental model of testicular torsion. Materials and Methods: The left testes of 8 peripubertal NZW rabbits (3-6 months) were subjected to 0, 15, 30, 60, 90, 120, and 180 min of ischemia by applying a clamp to the spermatic cord, followed by reperfusion. Another set of 8 rabbits was subjected to 60 min of ischemia and administered antioxidants (acetylsalicylic acid, ascorbic acid, allopurinol, quercetin, superoxide dismutase) before reperfusion. Both testes of 4 animals per group were harvested at 24 h and the remaining 4 at 3 months. Johnsen scores for spermatogenic activity and other changes were assessed histologically and these were compared with testicular malondialdehyde (MDA), a measure of free radical damage, assayed on testicular homogenates using the thiobarbiturate method. Results: In the 24-hour reperfusion group, apoptotic bodies and giant cells were more prominent in the seminiferous tubules of the left testes compared to the right, and were maximal after 90 min. In the 3-month reperfusion group, giant cells were absent, and apoptotic bodies were reduced in both testes. Testicular MDA showed an increase only in the left testes in the 24-hour reperfusion group, while the 3-month group showed increased MDA levels in both testes, but more on the left. The Johnsen score fell only to 8.0 in the left testes in the 24-hour reperfusion group, but dropped to 2.3 in the 3-month reperfusion group. Only in the 3-month reperfusion group, did antioxidant-treated animals show a fall in Johnsen scores in the left testes, regardless of the type of antioxidant. Conclusion: These findings confirm a role for reactive oxygen species (ROS) in damage to spermatogenic cells in both the ipsilateral and contralateral testes following torsion, with longer term effects in the torted testis. Currently available antioxidants do not provide any significant long-term protection against morphological damage to the testis by ROS generated in testicular torsion.

Original languageEnglish
Pages (from-to)258-263
Number of pages6
JournalUrologia Internationalis
Volume75
Issue number3
DOIs
Publication statusPublished - Oct 2005
Externally publishedYes

Fingerprint

Reperfusion Injury
Testis
Rabbits
Reperfusion
Antioxidants
Malondialdehyde
Spermatic Cord Torsion
Giant Cells
Free Radicals
Reactive Oxygen Species
Ischemia
Spermatic Cord
Allopurinol
Seminiferous Tubules
Quercetin
Aspirin
Ascorbic Acid
Superoxide Dismutase
Theoretical Models

Keywords

  • Antioxidant
  • Apoptotic bodies
  • Free radical
  • Giant cell
  • Ischemia
  • Testis, torsion
  • Torsion, testicular

ASJC Scopus subject areas

  • Urology

Cite this

Morphological responses of the rabbit testis to ischemic/reperfusion injury due to torsion. / Anim, J. T.; Kehinde, E. O.; Prasad, Asa; Varghese, R.

In: Urologia Internationalis, Vol. 75, No. 3, 10.2005, p. 258-263.

Research output: Contribution to journalArticle

Anim, J. T. ; Kehinde, E. O. ; Prasad, Asa ; Varghese, R. / Morphological responses of the rabbit testis to ischemic/reperfusion injury due to torsion. In: Urologia Internationalis. 2005 ; Vol. 75, No. 3. pp. 258-263.
@article{4564b7de2f7348ae9fa7fb6e4c09286c,
title = "Morphological responses of the rabbit testis to ischemic/reperfusion injury due to torsion",
abstract = "Objective: To investigate the morphological effects of free radical injury on spermatogenic cells in both testes of the rabbit experimental model of testicular torsion. Materials and Methods: The left testes of 8 peripubertal NZW rabbits (3-6 months) were subjected to 0, 15, 30, 60, 90, 120, and 180 min of ischemia by applying a clamp to the spermatic cord, followed by reperfusion. Another set of 8 rabbits was subjected to 60 min of ischemia and administered antioxidants (acetylsalicylic acid, ascorbic acid, allopurinol, quercetin, superoxide dismutase) before reperfusion. Both testes of 4 animals per group were harvested at 24 h and the remaining 4 at 3 months. Johnsen scores for spermatogenic activity and other changes were assessed histologically and these were compared with testicular malondialdehyde (MDA), a measure of free radical damage, assayed on testicular homogenates using the thiobarbiturate method. Results: In the 24-hour reperfusion group, apoptotic bodies and giant cells were more prominent in the seminiferous tubules of the left testes compared to the right, and were maximal after 90 min. In the 3-month reperfusion group, giant cells were absent, and apoptotic bodies were reduced in both testes. Testicular MDA showed an increase only in the left testes in the 24-hour reperfusion group, while the 3-month group showed increased MDA levels in both testes, but more on the left. The Johnsen score fell only to 8.0 in the left testes in the 24-hour reperfusion group, but dropped to 2.3 in the 3-month reperfusion group. Only in the 3-month reperfusion group, did antioxidant-treated animals show a fall in Johnsen scores in the left testes, regardless of the type of antioxidant. Conclusion: These findings confirm a role for reactive oxygen species (ROS) in damage to spermatogenic cells in both the ipsilateral and contralateral testes following torsion, with longer term effects in the torted testis. Currently available antioxidants do not provide any significant long-term protection against morphological damage to the testis by ROS generated in testicular torsion.",
keywords = "Antioxidant, Apoptotic bodies, Free radical, Giant cell, Ischemia, Testis, torsion, Torsion, testicular",
author = "Anim, {J. T.} and Kehinde, {E. O.} and Asa Prasad and R. Varghese",
year = "2005",
month = "10",
doi = "10.1159/000087805",
language = "English",
volume = "75",
pages = "258--263",
journal = "Urologia Internationalis",
issn = "0042-1138",
publisher = "S. Karger AG",
number = "3",

}

TY - JOUR

T1 - Morphological responses of the rabbit testis to ischemic/reperfusion injury due to torsion

AU - Anim, J. T.

AU - Kehinde, E. O.

AU - Prasad, Asa

AU - Varghese, R.

PY - 2005/10

Y1 - 2005/10

N2 - Objective: To investigate the morphological effects of free radical injury on spermatogenic cells in both testes of the rabbit experimental model of testicular torsion. Materials and Methods: The left testes of 8 peripubertal NZW rabbits (3-6 months) were subjected to 0, 15, 30, 60, 90, 120, and 180 min of ischemia by applying a clamp to the spermatic cord, followed by reperfusion. Another set of 8 rabbits was subjected to 60 min of ischemia and administered antioxidants (acetylsalicylic acid, ascorbic acid, allopurinol, quercetin, superoxide dismutase) before reperfusion. Both testes of 4 animals per group were harvested at 24 h and the remaining 4 at 3 months. Johnsen scores for spermatogenic activity and other changes were assessed histologically and these were compared with testicular malondialdehyde (MDA), a measure of free radical damage, assayed on testicular homogenates using the thiobarbiturate method. Results: In the 24-hour reperfusion group, apoptotic bodies and giant cells were more prominent in the seminiferous tubules of the left testes compared to the right, and were maximal after 90 min. In the 3-month reperfusion group, giant cells were absent, and apoptotic bodies were reduced in both testes. Testicular MDA showed an increase only in the left testes in the 24-hour reperfusion group, while the 3-month group showed increased MDA levels in both testes, but more on the left. The Johnsen score fell only to 8.0 in the left testes in the 24-hour reperfusion group, but dropped to 2.3 in the 3-month reperfusion group. Only in the 3-month reperfusion group, did antioxidant-treated animals show a fall in Johnsen scores in the left testes, regardless of the type of antioxidant. Conclusion: These findings confirm a role for reactive oxygen species (ROS) in damage to spermatogenic cells in both the ipsilateral and contralateral testes following torsion, with longer term effects in the torted testis. Currently available antioxidants do not provide any significant long-term protection against morphological damage to the testis by ROS generated in testicular torsion.

AB - Objective: To investigate the morphological effects of free radical injury on spermatogenic cells in both testes of the rabbit experimental model of testicular torsion. Materials and Methods: The left testes of 8 peripubertal NZW rabbits (3-6 months) were subjected to 0, 15, 30, 60, 90, 120, and 180 min of ischemia by applying a clamp to the spermatic cord, followed by reperfusion. Another set of 8 rabbits was subjected to 60 min of ischemia and administered antioxidants (acetylsalicylic acid, ascorbic acid, allopurinol, quercetin, superoxide dismutase) before reperfusion. Both testes of 4 animals per group were harvested at 24 h and the remaining 4 at 3 months. Johnsen scores for spermatogenic activity and other changes were assessed histologically and these were compared with testicular malondialdehyde (MDA), a measure of free radical damage, assayed on testicular homogenates using the thiobarbiturate method. Results: In the 24-hour reperfusion group, apoptotic bodies and giant cells were more prominent in the seminiferous tubules of the left testes compared to the right, and were maximal after 90 min. In the 3-month reperfusion group, giant cells were absent, and apoptotic bodies were reduced in both testes. Testicular MDA showed an increase only in the left testes in the 24-hour reperfusion group, while the 3-month group showed increased MDA levels in both testes, but more on the left. The Johnsen score fell only to 8.0 in the left testes in the 24-hour reperfusion group, but dropped to 2.3 in the 3-month reperfusion group. Only in the 3-month reperfusion group, did antioxidant-treated animals show a fall in Johnsen scores in the left testes, regardless of the type of antioxidant. Conclusion: These findings confirm a role for reactive oxygen species (ROS) in damage to spermatogenic cells in both the ipsilateral and contralateral testes following torsion, with longer term effects in the torted testis. Currently available antioxidants do not provide any significant long-term protection against morphological damage to the testis by ROS generated in testicular torsion.

KW - Antioxidant

KW - Apoptotic bodies

KW - Free radical

KW - Giant cell

KW - Ischemia

KW - Testis, torsion

KW - Torsion, testicular

UR - http://www.scopus.com/inward/record.url?scp=26444493462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=26444493462&partnerID=8YFLogxK

U2 - 10.1159/000087805

DO - 10.1159/000087805

M3 - Article

VL - 75

SP - 258

EP - 263

JO - Urologia Internationalis

JF - Urologia Internationalis

SN - 0042-1138

IS - 3

ER -