We tested the immunoreactivity of two mouse monoclonal antibodies (HMFG1 and HMFG2) raised against the membrane associated mucin MUC1, in bladder cancer. We have used an avidin-biotin immunoperoxidase technique to localise MUC1 in 80 microwave-treated, paraffin-embedded samples comprising of 70 transitional cell carcinomas (TCC) and 10 normal bladder biopsies. The results showed that HMFG1 and HMFG2 reacted with the majority of normal tissues in a membranous pattern, while a variant number of malignant tissues, increasing with the degree of malignancy, showed membranous and/or cytoplasmic staining. Both HMFG1 and HMFG2 localisation were positively correlated with tumour grade and muscle invasion. When HMFG2 was compared with HMFG1, the later showed a more homogeneous and intense pattern of immunostaining. Our results suggest that HMFG1 could be used as a carrier of toxic agents, for intravesical treatment of bladder cancer.
|Number of pages||1|
|Journal||British Journal of Urology|
|Issue number||SUPPL. 2|
|Publication status||Published - Dec 1 1997|
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