Multiple hormone storage by cells of the human pituitary

G. R. Newman, B. Jasani, E. D. Williams

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

While immunostaining serial semi-thin sections of acrylic resin-embedded normal human pituitary using antisera to human pituitary hormones, it became clear that several cells were stained by more that one antiserum. The tissue had been surgically excised from a patient with a prolactinoma. The tumor, which was immunoreactive only with antiprolactin antiserum, was distinctly different from the pieces of tissue under study which had normal pituitary architecture and demonstrated immunoreactivity with antisera against all six of the common pituitary hormones. A major immunoelectron microscopic investigation, using immunocolloidal gold and immunoperoxidase methods, revealed cells in which follicle-stimulating hormone (FSH), luteinizing hormone (LH), and prolactin (PRL) were co-localized to the same electron-dense granules. Some similar cells also possessed electron-lucent granules immunoreactive only for anti-PRL antiserum. Adrenocorticotrophic hormone (ACTH) and PRL were also found in the same cell but were very largely localized to separate, morphologically different populations of electron-dense and -lucent storage granules. By employing double immunolabeling, a few granules in the ACTH/PRL cells were shown to be immunoreactive to both anti-ACTH and anti-PRL antisera. The possibility that the multihormonal cells are syncytial or that they represent multipotential stem cells is discussed.

Original languageEnglish
Pages (from-to)1183-1192
Number of pages10
JournalJournal of Histochemistry and Cytochemistry
Volume37
Issue number8
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Immune Sera
Prolactin
Hormones
Adrenocorticotropic Hormone
Pituitary Hormones
Electrons
Acrylic Resins
Prolactinoma
Follicle Stimulating Hormone
Luteinizing Hormone
Gold
Stem Cells
Population
Neoplasms

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Newman, G. R., Jasani, B., & Williams, E. D. (1989). Multiple hormone storage by cells of the human pituitary. Journal of Histochemistry and Cytochemistry, 37(8), 1183-1192.

Multiple hormone storage by cells of the human pituitary. / Newman, G. R.; Jasani, B.; Williams, E. D.

In: Journal of Histochemistry and Cytochemistry, Vol. 37, No. 8, 1989, p. 1183-1192.

Research output: Contribution to journalArticle

Newman, GR, Jasani, B & Williams, ED 1989, 'Multiple hormone storage by cells of the human pituitary', Journal of Histochemistry and Cytochemistry, vol. 37, no. 8, pp. 1183-1192.
Newman, G. R. ; Jasani, B. ; Williams, E. D. / Multiple hormone storage by cells of the human pituitary. In: Journal of Histochemistry and Cytochemistry. 1989 ; Vol. 37, No. 8. pp. 1183-1192.
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