Mutations in the pncAand rpsAgenes among 77 Mycobacterium tuberculosis isolates in Kazakhstan

A. Akhmetova, U. Kozhamkulov, V. Bismilda, L. Chingissova, T. Abildaev, M. Dymova, M. Filipenko, E. Ramanculov

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

SETTING: Pyrazinamide (PZA), an important first-line drug for anti-tuberculosis treatment, demonstrates potent activity against semi-dormant bacilli in acidic environments. However, the diagnosis of PZA resistance is often impeded by technical difficulties. OBJECTIVE: To characterise mutations in the pncA and rpsA genes among PZA-resistant and PZA-susceptible clinical Mycobacterium tuberculosis isolates circulating in Kazakhstan. The potential use of genotyping to identify PZA resistance was also investigated. DESIGN: PZA drug susceptibility testing and pncA and rpsA gene sequencing were performed on 77 clinical M. tuberculosis isolates; mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRUVNTR) typing was performed on 74 clinical M. tuberculosis isolates. RESULTS: Of the 77 clinical M. tuberculosis isolates, 41 (53.2%) were phenotypically resistant to PZA, whereas 36 (46.7%) were susceptible; 48 (62.3%) of these isolates were also multidrug-resistant (MDR). Furthermore, 38 (49.3%) clinical isolates showed mutations in the pncA gene and its flanking region; the majority of these isolates (n = 36, 94.7%) were also MDR. Gene sequencing showed that only synonymous substitutions affecting rpsA occurred. MIRU-VNTR typing revealed that 78.4% of isolates were of the Beijing genotype. CONCLUSIONS: Sequencing revealed that mutations in pncA, but not in rpsA, occurred in PZA-resistant M. tuberculosis isolates circulating in the territory of Kazakhstan.

Original languageEnglish
Pages (from-to)179-184
Number of pages6
JournalInternational Journal of Tuberculosis and Lung Disease
Volume19
Issue number2
DOIs
Publication statusPublished - Feb 1 2015
Externally publishedYes

Keywords

  • Mycobacterium tuberculosis
  • PZA
  • Pyrazinamide
  • Resistance
  • Sequencing

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Mutations in the pncAand rpsAgenes among 77 Mycobacterium tuberculosis isolates in Kazakhstan'. Together they form a unique fingerprint.

  • Cite this