Nano-mole scale sequential signal assignment by 1H-detected protein solid-state NMR

Songlin Wang; Sudhakar Parthasarathy; Yiling Xiao; Yusuke Nishiyama; Fei Long; Isamu Matsuda; Yuki Endo; Takahiro Nemoto; Kazuo Yamauchi; Tetsuo Asakura; Mitsuhiro Takeda; Tsutomu Terauchi; Masatsune Kainosho; Yoshitaka Ishii

doi:10.1039/c5cc04618a

Nano-mole scale sequential signal assignment by ¹H-detected protein solid-state NMR

Songlin Wang, Sudhakar Parthasarathy, Yiling Xiao, Yusuke Nishiyama, Fei Long, Isamu Matsuda, Yuki Endo, Takahiro Nemoto, Kazuo Yamauchi, Tetsuo Asakura, Mitsuhiro Takeda, Tsutomu Terauchi, Masatsune Kainosho, Yoshitaka Ishii

Research output: Contribution to journal › Article

29 Citations (Scopus)

Abstract

We present a 3D ¹H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10-100 nmol of fully protonated proteins using ultra-fast magic-angle spinning (MAS) at ∼80 kHz by a novel spectral-editing method, which permits drastic spectral simplification. The approach offers ∼110 fold time saving over a traditional 3D ¹³C-detected SSNMR approach.

Original language	English
Pages (from-to)	15055-15058
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	81
DOIs	https://doi.org/10.1039/c5cc04618a
Publication status	Published - Aug 14 2015
Externally published	Yes

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ASJC Scopus subject areas

Chemistry(all)
Catalysis
Ceramics and Composites
Electronic, Optical and Magnetic Materials
Surfaces, Coatings and Films
Materials Chemistry
Metals and Alloys

Cite this

Wang, S., Parthasarathy, S., Xiao, Y., Nishiyama, Y., Long, F., Matsuda, I., Endo, Y., Nemoto, T., Yamauchi, K., Asakura, T., Takeda, M., Terauchi, T., Kainosho, M., & Ishii, Y. (2015). Nano-mole scale sequential signal assignment by ¹H-detected protein solid-state NMR. Chemical Communications, 51(81), 15055-15058. https://doi.org/10.1039/c5cc04618a

Nano-mole scale sequential signal assignment by ¹H-detected protein solid-state NMR. / Wang, Songlin; Parthasarathy, Sudhakar; Xiao, Yiling; Nishiyama, Yusuke; Long, Fei; Matsuda, Isamu; Endo, Yuki; Nemoto, Takahiro; Yamauchi, Kazuo; Asakura, Tetsuo; Takeda, Mitsuhiro; Terauchi, Tsutomu; Kainosho, Masatsune; Ishii, Yoshitaka.

In: Chemical Communications, Vol. 51, No. 81, 14.08.2015, p. 15055-15058.

Research output: Contribution to journal › Article

Wang, Songlin ; Parthasarathy, Sudhakar ; Xiao, Yiling ; Nishiyama, Yusuke ; Long, Fei ; Matsuda, Isamu ; Endo, Yuki ; Nemoto, Takahiro ; Yamauchi, Kazuo ; Asakura, Tetsuo ; Takeda, Mitsuhiro ; Terauchi, Tsutomu ; Kainosho, Masatsune ; Ishii, Yoshitaka. / Nano-mole scale sequential signal assignment by ¹H-detected protein solid-state NMR. In: Chemical Communications. 2015 ; Vol. 51, No. 81. pp. 15055-15058.

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10.1039/c5cc04618a